Subhuman Primate Diploid Cells: Possible Substrates for Production of Virus Vaccines

Petricciani, John C.; Hopps, Hope E.; Lorenz, Douglas E.

doi:10.1126/science.174.4013.1025

Cited by 20 publications

(9 citation statements)

References 5 publications

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“…FRhL-2 was nonpermissive to HSV; only human diploid cells were permissive to CMV. This confirmed the results reported by Petricciani et al (10). It is also relevant to refer that Kubicek et al (7) observed no CPE of HSV in fetal rhesus monkey foreskin cell (rhfs 2), and Nii (9) reported abortive infection of HSV in an established cell line of rhesus monkey kidney cell (LLC-MK2).…”

supporting

confidence: 87%

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Susceptibility of Primate Diploid Cells to Human Herpesviruses

Minamishima

Eizuru

1977

Microbiology and Immunology

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supporting

confidence: 87%

“…In fact, this was true in virological diagnosis of patients with eczema herpeticum, generalized herpes zoster and eczema vaccinatum (unpublished data). Vaccinia virus replicated in ERhL, as reported by Petricciani et al (10), producing intracytoplasmic inclusion bodies.…”

supporting

confidence: 54%

Susceptibility of Primate Diploid Cells to Human Herpesviruses

Minamishima

Eizuru

1977

Microbiology and Immunology

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“…Metaphases from a cell culture of Macaco mulatto in the 71st passage; note the difference in size between the chromosome with the achromatic region proximal to the centromere (single arrows) and those chromosomes in which the achromatic region is distal (double arrows), x 2,400. tions. Although diploid cell lines from some subhuman primate tissue have been propagated for as many as 36 passages [Petricciani et al, 1971;Coop er, 1972], our cultures were aneuploid and contained metaphases with al tered chromosomes by the 17th subculture. Focusing our attention on the evolution of the marked chromosomes in our cell line, we found them to be characterized by an easily recognizable achromatic region amply documented in several primate species.…”

Section: Discussionmentioning

confidence: 99%

Chromosomal Evolution in a Rhesus Cell Line

Pasztor¹,

Hu²

1973

IJFP

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Chromosomal evolution was examined in a long-term cell culture derived from Macaca mulatta eye tissue. In addition to the ‘marked’ chromosome that characterizes the rhesus karyotype, other chromosomes that may have originated from it by inversions and translocations were identified at each subculture sampled. Our observations do not warrant the conclusion that all the achromatically distinguished chromosomes observed were ‘marked’ rather than ‘marker’ chromosomes; some, however, resembled the ‘marked’ chromosomes found in Aotus and Cercopithecus.

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“…These strains were subsequently plaque purified and passaged in primary or secondary AGMK cells. The final passages of both animal strains were in DBS-FRhL-2 cells, a simian embryonic diploid cell strain (21). The RRV seed used for gene reassortment represented a later passage of lot RRV-1 (prepared at Flow Laboratories, Inc.) in DBS-FRhL-2 cells.…”

Section: Methodsmentioning

confidence: 99%

Single gene substitution rotavirus reassortants containing the major neutralization protein (VP7) of human rotavirus serotype 4

Midthun¹,

Hoshino²,

Kapikian³

et al. 1986

J Clin Microbiol

106

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A series of reassortants was isolated from coinfection of cell cultures with wild-type bovine rotavirus (UK strain [serotype 6]) or rhesus rotavirus (strain MMU18006 [serotype 3]) and a tissue culture-adapted human rotavirus strain, ST3 (serotype 4). Monospecific antiserum or a set of monoclonal antibodies to the major outer capsid neutralization glycoprotein, VP7, of the animal rotavirus parent was used to select for reassortants with human rotavirus serotype 4 neutralization specificity. The majority of reassortants contained only gene 9 of the human rotavirus parent, ST3, whereas the remaining genes were derived from the animal rotavirus parent. These single human rotavirus gene substitution reassortants were neutralized to high titer by hyperimmune serum directed at ST3, thus demonstrating that gene 9 of ST3 codes for the major neutralization protein of this strain. Moreover, these single gene substitution reassortants were also neutralized to low titer by antiserum directed at their animal rotavirus parent, probably because they derived gene 4, which codes for another outer capsid protein, VP3, from their animal rotavirus parent. None of the reassortants derived gene 4, which had previously been shown to be responsible for host range restriction of human rotaviruses in tissue culture, from ST3, despite the fact that the ST3 strain used for gene reassortment had been tissue culture adapted.

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Subhuman Primate Diploid Cells: Possible Substrates for Production of Virus Vaccines

Cited by 20 publications

References 5 publications

Susceptibility of Primate Diploid Cells to Human Herpesviruses

Susceptibility of Primate Diploid Cells to Human Herpesviruses

Chromosomal Evolution in a Rhesus Cell Line

Single gene substitution rotavirus reassortants containing the major neutralization protein (VP7) of human rotavirus serotype 4

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