Sublethal exposure of heavy metals induces micronuclei in fish, Channa punctata

Yadav, Khushboo; Trivedi, Sunil P.

doi:10.1016/j.chemosphere.2009.10.022

Cited by 86 publications

(48 citation statements)

References 45 publications

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“…Apart from MN, other nuclear anomalies like deformed nucleus, nuclear bud and vacuolated nucleus, binucleated cells were observed at sublethal exposure. Similar observations from our results are in agreement with other studies (Klobucar et al, 2012;Yadav and Trivedi, 2009). …”

Section: Genotoxicity Of Chromium In Catla Catla By Micronucleus Assaysupporting

confidence: 83%

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IN-VIVO EVALUATION OF HEXAVALENT CHROMIUM INDUCED DNA DAMAGE BY ALKALINE COMET ASSAY AND OXIDATIVE STRESS IN CATLA CATLA

Arunachalam

Annamalai

Kuruva

2013

American Journal of Environmental Sciences

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In the present study, the acute toxicity of Chromium in fingerlings of Catla catla, an Indian major carp, was evaluated with renewal bioassay method. In vivo studies were designed to assess the extent of Micronucleus Assay, Comet Assay under the exposure of common heavy-metal compounds, namely, Chromium Nitrate, using Catla catla (2n = 20), as a test model. The laboratory acclimatized fishes were divided into four groups. Group I served as positive control and the other three as exposed groups for three different time durations of 7, 14 and 21 days and were subjected to uninterrupted sub lethal concentrations (50% of 96 h LC 50 ). The experiments were planned in such a way that fish from all the groups were sacrificed on the same day. The frequencies of micronuclei and bi-nuclei were evaluated comparatively in peripheral erythrocytes. As a result, it was observed that, the fishes and different tissues showed differential sensitivity to the heavy-metal treatment. A significant increase in the frequencies of micronucleated and binucleated cells and percentage increase in DNA tail (p<0.001) through Alkaline Comet Assay were observed after 21 days of exposure to chromium. Our results also showed decrease in enzyme Superoxide Dismutase (SOD) activity and increase in catalase enzyme due to increasing chromium concentration. The bio-concentration factor profiles of Chromium in Catla catla during sub lethal toxicity study was also calculated.

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Section: Genotoxicity Of Chromium In Catla Catla By Micronucleus Assaysupporting

confidence: 83%

“…Group I (n = 20) served as positive control and the other three as exposed groups (n = 20) for three different time durations of 7, 14 and 21 days and were subjected to sublethal concentrations (50% of 96 h LC 50 ) (Quinn et al, 2006;Yadav and Trivedi, 2009). All the experiments were performed in duplicates.…”

Section: Experimental Design For Acute Toxicity Testsmentioning

confidence: 99%

IN-VIVO EVALUATION OF HEXAVALENT CHROMIUM INDUCED DNA DAMAGE BY ALKALINE COMET ASSAY AND OXIDATIVE STRESS IN CATLA CATLA

Arunachalam

Annamalai

Kuruva

2013

American Journal of Environmental Sciences

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“…The average frequency of MN in fishes exposed to As (III) 12.03 was reported by Yadav [14] in Channa punctatus, but in this case the average frequency of 13.10 which is when compared the reported fish. Strunjak-Perovic [15] suggested that evaluation of erythrocytes in triploid rainbow trout did no longer reveal the presence of any morphological NAs in spite of substantially better MN frequency in relation to ordinary, diploid fish suggesting that correlation between NAs and MN is an end result of clastogenic but not aneugenic effects.…”

Section: Velu and Uthandakalaipandianmentioning

confidence: 70%

Confinement of Micronucleus on Dawkinsia Tambraparniei by the Paradoxical Effect of the Arsenic Derivatives

Velu

Uthandakalaipandian

2017

Asian J Pharm Clin Res

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Objective: In the current study, in vivo genotoxic effects of arsenic derivatives such as arsenic trioxide (As 2 O 3 ) and sodium arsenite (NaAsO 2 ) on peripheral blood erythrocytes of Dawkinsia tambraparniei were investigated using the micronucleus (MN) test.Methods: MN staining was done using acridine orange pre-coated slides. Fluorescent microscope was used for scoring. Results:In NaAsO 2 exposed, the erythrocytes highest value was recorded at 42 days which is twofold higher than exposure at 7 days when compared to As 2 O 3 . Highest percentage was recorded about 13.9 in NaAsO 2 , and in case of As 2 O 3 , it was recorded as 0.2% less. It was clearly confirmed that either form of arsenic is toxic to organism. Conclusion:Anthropogenic activities have also brought in substantial amounts of them into the environment by mobilization from their natural insoluble deposits or environmental sins. Hence, arsenic pollution should have measured, and arsenic removal process should have carried out.

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“…The contamination of water resources by As, mainly in the eastern hemisphere, is one of the most relevant environmental concern at the global scale (Frisbie et al 2002;Ahmed et al 2011a). Mining and industrial waste largely facilitate the entering of arsenic compounds into the environmental compartments and the human exposition (Yadav and Trivedi 2009). Both organic and inorganic compounds are present in the environment, the last being more toxic (Frisbie et al 2002;Ahmed et al 2011a).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, the use of histological, biochemical, cytogenetic, and other biomarkers is increasingly required and applied in environmental monitoring and protection. Vertebrate are suitable models to estimate possible risks in the aquatic environment due to their ability to efficiently metabolize, concentrate, and store waterborne pollutants (Yadav and Trivedi 2009;Cavas 2011). Above all, fish are excellent specimens for the study of the mutagenic or carcinogenic potential because they are particularly sensitive to pollutants that interact with DNA (Schnurstein and Braunbeck 2001).…”

Section: Introductionmentioning

confidence: 99%

Early genotoxic response and accumulation induced by waterborne copper, lead, and arsenic in European seabass, Dicentrarchus labrax

Canalejo

Díaz-de-Alba

Granado-Castro

et al. 2015

Environ Sci Pollut Res

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Cu, Pb, and As, which are among the most abundant metals in the aquatic environment, are also among the most health-threatened by causing diverse cellular injuries. The aim of this study was to assess and compare the potential early induction of genotoxic effects after waterborne Cu, Pb, and As exposure in European seabass, Dicentrarchus labrax, a commercial widely cultured fish, using the micronucleus (MN) assay in peripheral blood erythrocytes. Fish were exposed under laboratory conditions to nominal solutions ranging 0-10 mg/L for 24 and 96 h. Furthermore, actual metal ion concentrations were measured by inductively coupled plasma atomic emission spectroscopy (ICP-AES) or differential pulse anodic stripping voltammetry (DPASV) in water and four fish tissues differentially related to environmental exposition and metal accumulation, i.e. the gills, liver, muscle, and brain. Dose-dependent increases of micronuclei (MNi) frequency were observed after these very short exposures; based on measured metal concentrations in water, the genotoxic effect ordered as Cu > As > Pb. Significant genotoxic effect at 0.009 mg/L Cu, 0.57 mg/L Pb, and 0.01 mg/L As was seen. For Cu and Pb these are only slightly higher, but for As it is notably lower than the USEPA criteria of maximum concentration to prevent acute toxicity in aquatic organisms.Furthermore, genotoxicity was differentially related to metal accumulation. MNi frequency correlated positively with the content of Pb in all the organs, with the content of As in liver and gills and only with the content of Cu in the brain. In conclusion, our findings raised environmental concerns because these depicted a genotoxic potential of Cu, Pb, and As after a very short exposure to low but environmentally relevant concentrations, too close to regulatory thresholds. In addition, the MN test in D. labrax could be considered an early biomarker of genotoxicity induced by these metals in fish.

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Sublethal exposure of heavy metals induces micronuclei in fish, Channa punctata

Cited by 86 publications

References 45 publications

<i>IN-VIVO</i> EVALUATION OF HEXAVALENT CHROMIUM INDUCED DNA DAMAGE BY ALKALINE COMET ASSAY AND OXIDATIVE STRESS IN <i>CATLA CATLA</i>

<i>IN-VIVO</i> EVALUATION OF HEXAVALENT CHROMIUM INDUCED DNA DAMAGE BY ALKALINE COMET ASSAY AND OXIDATIVE STRESS IN <i>CATLA CATLA</i>

Confinement of Micronucleus on Dawkinsia Tambraparniei by the Paradoxical Effect of the Arsenic Derivatives

Early genotoxic response and accumulation induced by waterborne copper, lead, and arsenic in European seabass, Dicentrarchus labrax

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