Submerged fermentation of wheat bran by Aspergillus flavus for production and characterization of carboxy methyl cellulase

Gomathi, D.; Muthulakshmi, C; Kumar, Dinesh; Ravikumar, Ganesan; Kalaiselvi, M.; Uma, C.

doi:10.1016/s2221-1691(12)60132-4

Cited by 47 publications

(22 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the proposed optimal conditions differed between studies due to different sources of isolates (water, soil, decaying plants) and inherent production capacity of the isolates. Among the different types of fungi, A. flavus was favored for cellulase production (Gomathi et al, 2012;Utharalakshmi et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Expression of genes encoding cellulolytic enzymes in some Aspergillus species

et al. 2016

View full text Add to dashboard Cite

ABSTRACT. Fermentation is an important industrial process for microbial metabolite development and has wide applications in various fields. Aspergillus is the most important genus of fungi used for the production of microbial enzymes such as cellulase. The Aspergillus genome encodes various cellulolytic enzymes. In this study, we assayed the gene expression and cellulolytic enzyme production of three isolates: A. niger (KSU009), A. terreus (KC462061), and A. flavus (KSU014). Two fermentation systems, submerged fermentation and biofilm fermentation (BF), were used for this purpose. Gene expression analysis by RT-PCR showed that cbhB, exo, eglA, eglB, eglC, and β-actin genes were differentially expressed in the two fermentation systems for these three isolates during enzyme production. Furthermore, the expression of all genes was found to be higher in the BF system. The six genes were not expressed in the isolates with no cellulolytic enzyme production. The isolates were identified by morphological and molecular methods, which were based on macroscopic characteristics and sequence analysis of ITS1, ITS2, and the 5.8S regions of rDNA.

show abstract

Section: Discussionmentioning

confidence: 99%

Expression of genes encoding cellulolytic enzymes in some Aspergillus species

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The β-glucosidase of Urea and Ulva cocktails displayed over a broad range of pH values, from 2 to 7 and from 3 to 8, respectively, with a maximum activity at pH 4.8 for both cocktails. 44 On the other hand, β-glucosidase of Ulva cocktail, presented a high activity at pH 6 and 8 with a low activity at pH 10. This pH offers an advantage for OFMSW hydrolysis, which is an acid substrate.…”

Section: Optimum Ph and Temperaturementioning

confidence: 98%

“…This could be related to the growth of microorganism that required a slightly acidic pH (acid medium). 44 On the other hand, β-glucosidase of Ulva cocktail, presented a high activity at pH 6 and 8 with a low activity at pH 10.…”

Section: Optimum Ph and Temperaturementioning

confidence: 98%

Production and characterization of β‐glucosidase from Aspergillus niger fermentation: Application for organic fraction of municipal solid waste hydrolysis and methane enhancement

Mlaik

Sayadi

Hamza

et al. 2019

Biotechnology Progress

View full text Add to dashboard Cite

The anaerobic digestion of the organic fraction of municipal solid waste (OFMSW) is currently an attractive treatment process with energy production in the form of biogas. Hydrolysis is the rate-limiting step for the anaerobic digestion of solid wastes.Thus, in the present study fungal enzymatic pretreatment of OFMSW was applied to enhance biogas production. Two enzyme cocktails rich on β-glucosidase were produced from submerged fermentation of Aspergillus niger on basal medium using OFMSW as carbon source and urea (Urea cocktail) and Ulva rigida as nitrogen source (Ulva cocktail). Ulva cocktail displayed an important effect on OFMSW solubilization. Therefore, an increase of reducing sugar concentration about 60% was obtained which was in correlation with chemical oxygen demand (COD) increase. The performance of enzymatic pretreatment on anaerobic digestion of OFMSW was studied by conducting biochemical methane potential tests. Results showed that the enzymatic pretreatment improved methane yield of OFMSW even at high solid concentration.High methane yield about 500 ml/g total volatile solid was obtained, which corresponds up to 68% enhancement over the control. K E Y W O R D Sanaerobic digestion, enzymatic hydrolysis, enzyme cocktail, methane yield, organic fraction of municipal solid waste

show abstract

“…Several investigations have been done into SF using agro waste such as corn stover, bagasse, and husk (Nema et al, 2015;Oguntimein et al, 1992); de--seeded sunflower head (Patil and Dayanand, 2006); residual pant biomass from industrial processing of Artemisia annua (Chandra et al, 2010); and wheat straw and barn (Gomathi et al, 2012;Singh et al, 2009) as a carbon source.…”

Section: Jatropha Curcas L Is a Tropical Plant That Is Used For Sevementioning

confidence: 99%

Utilisation of Jatropha press cake as substrate in biomass and lipase production from Aspergillus niger 65I6 and Rhizomucor miehei CBS 360.62

Ilmi

Hidayat

Hastuti

et al. 2017

Biocatalysis and Agricultural Biotechnology

View full text Add to dashboard Cite

Submerged fermentation of wheat bran by Aspergillus flavus for production and characterization of carboxy methyl cellulase

Cited by 47 publications

References 16 publications

Expression of genes encoding cellulolytic enzymes in some Aspergillus species

Expression of genes encoding cellulolytic enzymes in some Aspergillus species

Production and characterization of β‐glucosidase from Aspergillus niger fermentation: Application for organic fraction of municipal solid waste hydrolysis and methane enhancement

Utilisation of Jatropha press cake as substrate in biomass and lipase production from Aspergillus niger 65I6 and Rhizomucor miehei CBS 360.62

Contact Info

Product

Resources

About