SUBMICROSCOPIC PARTICLES IN EXTRACTS OF
            <i>AZOTOBACTER AGILIS</i>

Cota-Robles, E. H.; Marr, Allen G.; Nilson, E.H.

doi:10.1128/jb.75.3.243-252.1958

Cited by 62 publications

(14 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We know, furthermore, from the researches of Palade and Siekevitz (26,27), that particles similar in size to the R particles have been found both in situ and in cell fractions from basophile cytoplasms, and chemical analyses have revealed that these particles are ribonucleoproteins. Particles of this size and chemical composition have now been found in cell fractions from various sources by several other authors (9,11,35,38,39).…”

Section: Discussionmentioning

confidence: 90%

“…Observations made on material extracted from animal cells by Palade and Siekevitz (26,27), from pea seedlings by Ts'o el al. (39) as well as from bacteria (11,35,38) and yeasts (9), have shown the existence of a particulate component formed of ribonucleoproteins. As pointed out recently by Tissi4res and Watson (38), the particles extracted from these varied sources show a similarity in sedimentation coefficient, size, and chemical composition.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Relation Between Basophilia and Fine Structure of Cytoplasm in the Fungus Allomyces macrogynus Em

Blondel

Turian

1960

The Journal of Cell Biology

View full text Add to dashboard Cite

In a fungus, illomyces macrogynus Em., staining tests have revealed changes in the location of cytoplasmic basophilia following different phases of the developmental cycle. These variations in location were used to observe which fine structures correspond to basophile and non-basophile areas of the cytoplasm.Hyphae, gametangia, zygotes, and plants were fixed at various developmental stages in OsO4, pH 6.1, and embedded in vestopal. Sections were examined in the electron microscope.Comparison of basophile and non-basophile cytoplasms leads to the conclusion that cytoplasmic particles of 150 to 200 A in diameter are responsible for basophilia. The possibility of these particles being ribosomes is discussed and confirmed.The present paper also describes some observations on the fine structure of other cellular components of this fungus, such as nuclei, mitochondria, various granules, and flagella.

show abstract

Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Relation Between Basophilia and Fine Structure of Cytoplasm in the Fungus Allomyces macrogynus Em

Blondel

Turian

1960

The Journal of Cell Biology

View full text Add to dashboard Cite

show abstract

“…Any study of the synthesis of protein raises the problem of obtaining a constant fraction that is essentially free of particles that are not chromatophores. Three types of purification procedure have been attempted: (a) partition between two immiscible aqueous phases (Albertsson, 1961); (b) electrophoresis in a starch slurry under the conditions of Cota-Robles, Marr & Nilson (1958); (c) densitygradient centrifuging with a sucrose-layer gradient. The last named gave the most reproducible results.…”

Section: Discussionmentioning

confidence: 99%

The association of protein synthesis with formation of pigments in some photosynthetic bacteria

Bull¹,

Lascelles²

1963

Biochemical Journal

View full text Add to dashboard Cite

NUCLEIC ACID METABOLISM IN DIVIDING LIVER 15 are not so marked as those occurring in young and foetal liver. This is to be expected by analogy with thymine catabolism. The effect of X-radiation on thymine catabolism in regenerating liver shows that, under conditions in which the formation of thymidine kinase is delayed, a delay in the depression of thymine catabolism also occurs. It is possible that the depression in thymine catabolism may be influenced by the appearance of thymidine kinase which occurs at about 18 hr. after partial hepatectomy (Bollum & Potter, 1959). When the formation of thymidine kinase is inhibited by X-radiation, the depression in thymine catabolism is delayed. SUMMARY 1. The activities of enzymes involved in nucleic acid metabolism in foetal, young and regenerating rat liver were compared with those in normal rat liver. 2. Thymidine phosphorylase and uridine phosphorylase were less active in foetal and young liver than in adult liver. Thymidine-phosphorylase activity was also reduced in regenerating liver. 3. Thymidine was incorporated into DNA in vitro more rapidly in the liver of foetal and young rats than in adult rats. 4. Dihydro-orotase activity was maximal in the liver at birth when it was twice as active as in the adult. 5. Total-body X-radiation (400 r.), when given to rats 6 hr. after partial hepatectomy, delayed the decrease in thymine catabolism.

show abstract

“…Solutions in 0-05m.phosphate buffer were also examined in the Tiselius electrophoresis apparatus. Although much light was scattered by the solution, causing the schlieren diagrams to blur, it was possible to show that the main component of paper electrophoresis could be resolved into two peaks to give a diagram similar to that obtained by Cota-Robles, Marr & Nilson (1958) in their studies with Azotobacter.…”

Section: I959mentioning

confidence: 57%

A ribonucleoprotein component of Escherichia coli

Bowen

Dagley

Sykes

1959

Biochemical Journal

View full text Add to dashboard Cite

METABOLISM OF ACETALDEHYDE 419 ethanol metabolism in the living organism condensation reactions of acetaldehyde can be of no quantitative importance. 5. Addition of pyruvate or other keto acids reduced the ethanol formation to a negligible value. It is therefore concluded that rat liver does not contain any true aldehyde mutase. 6. The ethanol formation is frequently larger than the aldehyde dehydrogenation. Evidence is obtained that this is due to reduced diphosphopyridine nucleotide, formed at a considerable rate from substrates present in the liver. 7. The concentration of diphosphopyridine nucleotide and reduced diphosphopyridine nucleotide during aldehyde metabolism was followed and the significance of the results is discussed. 8. Addition of pyruvate increased the rate of aldehyde dehydrogenation by about 50 %. It is suggested that this result may be caused by the formation of a complex between aldehyde dehydrogenase, lactic dehydrogenase and reduced diphosphopyridine nucleotide, which could be oxidized by pyruvate. The dissociation of reduced diphosphopyridine nucleotide from the aldehyde dehydrogenase, which is presumably the rate-limiting step, would in this way be circumvented. This investigation has been partially supported through a grant (to F. L.) from Statens almindelige Videnskabsfond.

show abstract

SUBMICROSCOPIC PARTICLES IN EXTRACTS OF AZOTOBACTER AGILIS

Cited by 62 publications

References 22 publications

Relation Between Basophilia and Fine Structure of Cytoplasm in the Fungus Allomyces macrogynus Em

Relation Between Basophilia and Fine Structure of Cytoplasm in the Fungus Allomyces macrogynus Em

The association of protein synthesis with formation of pigments in some photosynthetic bacteria

A ribonucleoprotein component of Escherichia coli

Contact Info

Product

Resources

About