2016
DOI: 10.1016/j.proeng.2016.11.224
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Subnanogram Detection of Silver Stained Protein Bands with Thermal Lens Spectrometry

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“…While TLM seems to be on the top in terms of method development and new applications, thermal lensing spectrometry (spectroscopy) is still used and developed as well. For instance, ultra-small particle concentration has been detected by Mazza et al [87,88], who evidenced the possibility of detecting with TLS the presence of 0.05 ng of protein bands in polyacrylamide gels soaked in methanol/ water and of 0.1 ng in gels soaked in water. As claimed by the authors, it has been by an order of magnitude smaller mass than detectable by other methods.…”
Section: Advances In Tlm and Tlsmentioning
confidence: 99%
“…While TLM seems to be on the top in terms of method development and new applications, thermal lensing spectrometry (spectroscopy) is still used and developed as well. For instance, ultra-small particle concentration has been detected by Mazza et al [87,88], who evidenced the possibility of detecting with TLS the presence of 0.05 ng of protein bands in polyacrylamide gels soaked in methanol/ water and of 0.1 ng in gels soaked in water. As claimed by the authors, it has been by an order of magnitude smaller mass than detectable by other methods.…”
Section: Advances In Tlm and Tlsmentioning
confidence: 99%