Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens

Abstract

Zinc is required for normal bone development and cartilage formation. The purpose of this study was to assess the effect of with adding organic Zn (alone or phytase inclusion) at the reduced dose to growing male Ross 308 chickens on somatic axis and bone tissue metabolism. 200 one-day old broilers were divided into the negative control group fed diet without Zn or phytase inclusion, positive control group receiving Zn in the 100% of daily recommended dose from ZnO, and two experimental groups fed diet introduced Zn in 25% of daily recommendation as a glycine chelate (Zn-Gly) with or without phytase inclusion (500 FTUÁkg -1 ). Supplemental organic Zn increased bone Zn and Mg content, serum IGF-1, growth hormone and leptin concentration. Additional phytase inclusion increased body weight gain, blood plasma Ca, Fe, Zn and osteocalcin concentration and tibia ash percentage when compared to the Zn-deprived control. Bone geometry, yield and ultimate strengths were enhanced in both organic Zn supplemented groups, and the overall mechanical strength parameters of bone were better in these groups than in the positive control group supplemented with standard dose of inorganic Zn. Also marked improvements in the thickness of articular and the growth plate cartilages as well as real bone volume and thickness of metaphyseal trabeculae were achieved in all broilers fed Zn-supplemented diet irrespective of phytase inclusion, however, the highest cancellous bone mass and the best trabecular structure were noted after ZnO supplementation. In concludion, although dietary organic Zn given to growing broilers in 25% of daily recommended dose improved general bone properties and mechanical strength, the obtained results do not allow to unambiguously state that organic Zn supplementation at this level, even after phytase inclusion, is sufficient for proper bone development.

The effect of bee pollen on bone biomechanical strength and trabecular bone histomorphometry in tibia of young Japanese quail (Coturnix japonica)

“…Besides calcium, other minerals are present in bee pollen (Table 2). Zinc plays important roles in bone metabolism as a catalyst of many enzymes that affect mineralization, such as bone alkaline phosphatase [53][54][55][56]. While alkaline phosphatase activity was not measured in the present study, Yamaguchi et al [5] showed that dietary bee pollen can promote alkaline phosphatase activity in metaphyseal tissues of bones, which, with increased mineralization, point to enhanced remodeling of trabecular bone.…”

Analysis of bone osteometry, mineralization, mechanical and histomorphometrical properties of tibiotarsus in broiler chickens demonstrates a influence of dietary chickpea seeds (Cicer arietinum L.) inclusion as a primary protein source

“…Bone remodeling and maturation, which involves changes in bone size and shape, depend on the interaction between bone cells activities, intermolecular networks of collagen, and interactions of proteoglycans and non-collagenous proteins. Poorly designed diet may influence these processes and lead to severe skeletal disorders [3, 5–9].…”

Effects of Yeast (Saccharomyces Cerevisiae) Probiotics Supplementation on Bone Quality Characteristics in Young Japanese Quail (Coturnix Japonica): The Role of Sex on the Action of the Gut-Bone Axis

“…Copper, zinc, manganese and magnesium are essential cofactors for enzymes involved in collagen synthesis and other bone matrix constituents that are required in bone and cartilage formation processes [48,49]. Thus, this enhanced mineral bioavailability may be directly related to the observed improvements in trabecular bone microarchitecture and articular cartilage histomorphometry, as has been reported in numerous previous studies on poultry, by our laboratory [50][51][52][53].…”