Subsetting reveals CD16<sup>high</sup> CD62L<sup>dim</sup> neutrophils in chronic rhinosinusitis with nasal polyps

Arebro, Julia; Drakskog, Cecilia; Winqvist, Ola; Bachert, Claus; Georén, Susanna Kumlien; Cardell, Lars Olaf

doi:10.1111/all.13919

Cited by 19 publications

(23 citation statements)

References 9 publications

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“…Ideally, these biomarkers should be supported by a body of evidence clarifying the biological significance, be quantifiable in a cost-efficient way and be easily measurable, preferably in blood or nasal secretion. 14 Potential biomarkers could be eosinophils, neutrophils, 135,136 IgE, 137 Th2 cytokines, 138 innate (epithelial) cytokines, 111,137,139 but also phenotypical phenomena like smell loss, 140 asthma and response to systemic corticosteroids. 134 Contrary to FeNO in asthma, nasal NO has not been shown to be helpful to identify the T2 endotype because the main source of production of nasal NO are the sinuses that are closed off when CRS occurs.…”

Section: Biomarkers Of Viral Infections In Exacerbation Of Allergic Rmentioning

confidence: 99%

Biomarkers in the Diagnosis and Therapy of Allergic Diseases and Asthma

Breiteneder¹,

Peng²,

Agache³

et al. 2020

Preprint

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Modern healthcare requires a proactive and individualized response to diseases, combining precision diagnosis and personalized treatment. Accordingly, the approach to patients with allergic diseases encompasses novel developments in the area of personalized medicine, disease phenotyping and endotyping and the development and application of reliable biomarkers. A detailed clinical history and physical examination followed by the detection of IgE immunoreactivity against specific allergens still represents the state of the art. However, nowadays, further emphasis focuses on the optimization of diagnostic and therapeutic standards and a large number of studies have been investigating the biomarkers of allergic diseases, including asthma, atopic dermatitis, allergic rhinitis, food allergy, urticaria and anaphylaxis. Various biomarkers have been developed by omics technologies, some of which lead to a better classification of the distinct phenotypes or endotypes. The introduction of biologicals to clinical practice increases the need for biomarkers for patient selection, prediction of outcomes and monitoring, to allow for an adequate choice of the duration of these costly and long-lasting therapies. Escalating healthcare costs together with questions on the efficacy of the current management of allergic diseases requires further development of a biomarker-driven approach. Here, we review biomarkers in diagnosis and treatment of asthma, atopic dermatitis, allergic rhinitis, viral infections, chronic rhinosinusitis, food allergy, drug hypersensitivity and allergen-immunotherapy with a special emphasis on specific IgE, microbiome and epithelial barrier. In addition, EAACI guidelines on biologicals are discussed within the perspective of biomarkers.

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Section: Biomarkers Of Viral Infections In Exacerbation Of Allergic Rmentioning

confidence: 99%

Biomarkers in the Diagnosis and Therapy of Allergic Diseases and Asthma

Breiteneder¹,

Peng²,

Agache³

et al. 2020

Preprint

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“…In other studies, neutrophils were found to be a major source of transforming growth factor‐beta2 (TGF‐β2) and oncostatin M, cytokines that induced sinonasal epithelial remodelling and epithelial barrier dysfunction, respectively, in CRS . Arebro et al demonstrated that three sets of neutrophils existed, stratified according to high or diminished expression of FcγRIII (CD16) and L‐selectin (CD62L). The neutrophil subset CD16 high CD62L dim , thought to be a more activated form of neutrophil with increased ability to phagocytose microbes, was significantly higher in nasal polyps compared to controls.…”

Section: Precision Medicine In Crsmentioning

confidence: 99%

Novel findings in immunopathophysiology of chronic rhinosinusitis and their role in a model of precision medicine

Ong

Wang

2019

Allergy

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Our understanding of the pathophysiology of chronic rhinosinusitis (CRS) is continuously evolving. The traditional description of CRS in terms of two phenotypes based on the presence or absence of nasal polyps belies the underlying intricate immunopathophysiological processes responsible for this condition. CRS is being increasingly recognized as a disease spectrum encompassing a range of inflammatory states in the sinonasal cavity, with non-type 2 inflammatory disease on one end, type 2 inflammatory, eosinophil-heavy disease on the other and an overlap of both in different proportions in between. Abundance in research on the immune mechanisms of CRS has revealed various new endotypes that hold promise as biomarkers for the development of targeted therapies in severe, uncontrolled CRS. The introduction of precision medicine to manage this chronic, complex condition is a step forward in providing individualized care for all patients with CRS. In this review, the latest research on the pathophysiology of CRS with a focus on potential novel biomarkers and treatment options over the last 2 years are summarized and integrated into a suggested model of precision medicine in CRS. K E Y W O R D S biomarkers, chronic rhinosinusitis, endotypes, immunopathophysiology, precision medicine

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“…Since all cholesteatoma matrix is removed during surgery it is likely that contributing factors to disease development reside outside the cholesteatoma matrix itself. For other conditions, like nasal polyps, it has been shown that the apparent healthy tissue adjacent to the diseased tissue also contains disease characteristics on a molecular level and contributes to disease pathology [12,13]. It is therefore of interest to examine the properties of the mucosa (other than perimatrix), in ears with cholesteatoma.…”

Section: Introductionmentioning

confidence: 99%

Extensive qPCR analysis reveals altered gene expression in middle ear mucosa from cholesteatoma patients

Drakskog¹,

Klerk²,

Westerberg³

et al. 2020

PLoS ONE

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The middle ear is a small and hard to reach compartment, limiting the amount of tissue that can be extracted and the possibilities for studying the molecular mechanisms behind diseases like cholesteatoma. In this paper 14 reference gene candidates were evaluated in the middle ear mucosa of cholesteatoma patients and two different control tissues. ACTB and GAPDH were shown to be the optimal genes for the normalisation of target gene expression when investigating middle ear mucosa in multiplex qPCR analysis. Validation of reference genes using c-MYC expression confirmed the suitability of ACTB and GAPDH as reference genes and showed an upregulation of c-MYC in middle ear mucosa during cholesteatoma. The occurrence of participants of the innate immunity, TLR2 and TLR4, were analysed in order to compare healthy middle ear mucosa to cholesteatoma. Analysis of TLR2 and TLR4 showed variable results depending on control tissue used, highlighting the importance of selecting relevant control tissue when investigating causes for disease. It is our belief that a consensus regarding reference genes and control tissue will contribute to the comparability and reproducibility of studies within the field.

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Subsetting reveals CD16^high CD62L^dim neutrophils in chronic rhinosinusitis with nasal polyps

Cited by 19 publications

References 9 publications

Biomarkers in the Diagnosis and Therapy of Allergic Diseases and Asthma

Biomarkers in the Diagnosis and Therapy of Allergic Diseases and Asthma

Novel findings in immunopathophysiology of chronic rhinosinusitis and their role in a model of precision medicine

Extensive qPCR analysis reveals altered gene expression in middle ear mucosa from cholesteatoma patients

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Subsetting reveals CD16high CD62Ldim neutrophils in chronic rhinosinusitis with nasal polyps

Cited by 19 publications

References 9 publications

Biomarkers in the Diagnosis and Therapy of Allergic Diseases and Asthma

Biomarkers in the Diagnosis and Therapy of Allergic Diseases and Asthma

Novel findings in immunopathophysiology of chronic rhinosinusitis and their role in a model of precision medicine

Extensive qPCR analysis reveals altered gene expression in middle ear mucosa from cholesteatoma patients

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Product

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Subsetting reveals CD16^high CD62L^dim neutrophils in chronic rhinosinusitis with nasal polyps