Substance P receptor expression in human skin keratinocytes and fibroblasts

Abstract

HaCaT cells and fibroblasts can express NK-1R at protein and transcription levels, and the expression was modulated by SP, IFN-gamma and Spantide I. This indicates that keratinocytes and fibroblasts are involved in the regulation of skin immunity and that NK-1R may play an important role in the pathogenesis of CAI.

The action of CGRP and SP on cultured skin fibroblasts

“…Three new articles were retrieved this way, for a total of 13 articles concerning the action of CGRP and/or SP on cultured human skin fibroblasts, of which 12 were related to SP and 1 was related to both CGRP and SP. A summary of the content of these articles is shown in Table 1 [30,[50][51][52][53][54][55][56][57][58][59][60][61]. A schematic drawing is proposed showing the molecular mechanisms of CGRP and SP in human skin fibroblasts and is shown in Figure 1.…”

“…SP can be a possible factor in the pathogenesis of cutaneous allergic inflammation. An investigation of the expression of NK-1R for SP in cultured human dermal fibroblasts and epidermal keratinocytes has reported that fibroblasts and keratinocytes can express NK-1R at both protein and transcriptional levels, and that this expression is upregulated by SP, gamma-interferon (IFN-gamma) and spantide I [55]. This suggests that fibroblasts and keratinocytes may be involved in the regulation of skin immune responses, and that NK-1R may play an important role in the pathogenesis of cutaneous allergic inflammation [55].…”

Probing the Effects of Stress Mediators on the Human Hair Follicle

“…3,27,28,31,54 Moreover, human keratinocytes and scalp HFs express NK1 receptors. 56 In culture, substance P stimulates proliferation of keratinocytes 57 and organ-cultured murine epidermis. 58 That substance P inhibited hair matrix keratinocyte proliferation in our assay, rather than stimulating it, underscores the very distinct, often underestimated, regulatory controls of HF versus epidermal keratinocytes.…”

Modulation of semaphorin 3A expression by calcium concentration and histamine in human keratinocytes and fibroblasts

“…In addition, C-fiber plays pluripotential roles for itch-related events. For example, neuropeptides released from C-fibers stimulate keratinocytes and mast cells to produce pruritogenic factors that again stimulate C-fiber [3][4][5].…”