The action of CGRP and SP on cultured skin fibroblasts

Hochman, Bernardo; Tucci-Viegas, Vanina Monique; Monteiro, Paola K. P.; França, Jerônimo Pereira de; Gaiba, Silvana; Ferreira, Lydia Masako

doi:10.2478/s11535-014-0301-6

Cited by 3 publications

(3 citation statements)

References 68 publications

(106 reference statements)

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“…For the concentrations of 1 × 10 −8 , 1 × 10 −7 and 1 × 10 −6 M, the free and encapsulated SP stimulated cell proliferation in a manner not significantly different (within 15%) than in the untreated controls. This finding conflicts with earlier studies, which observed that SP can promote proliferation of spinal cord-derived neural stem cells [ 59 ], bone marrow stromal cells, [ 15 ] and fibroblasts [ 18 ]. Further, a previous study showed that SP stimulated cellular proliferation of keratinocytes in vitro in a concentration-dependent manner when SP was added in the concentration range of 1 × 10 −10 to 1 × 10 −7 M [ 11 ].…”

Section: Resultscontrasting

confidence: 99%

“…In the skin, SP has been related to the neurogenic inflammation, as it stimulates the regeneration of wounds via inducing the release of nerve growth factors [ 17 ], intensifying the migration of keratinocytes [ 14 ] and stimulating the proliferation of fibroblasts [ 18 , 19 ]. Improving our understanding on the action of SP and its effects on skin wound healing could be of relevance, for example, in the treatment of diabetic wounds.…”

Section: Introductionmentioning

confidence: 99%

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A Chitosan—Based Liposome Formulation Enhances the In Vitro Wound Healing Efficacy of Substance P Neuropeptide

et al. 2017

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Currently, there is considerable interest in developing innovative biodegradable nanoformulations for controlled administration of therapeutic proteins and peptides. Substance P (SP) is a neuropeptide of 11 amino acids that belongs to the tachykinins family and it plays an important role in wound healing. However, SP is easily degradable in vivo and has a very short half-life, so the use of chitosan-based nanocarriers could enhance its pharmaceutical properties. In light of the above, the aim of this work was to produce and characterize chitosan-coated liposomes loaded with SP (SP-CH-LP) as novel biomaterials with potential application in mucosal wound healing. The loaded system’s biophysical properties were characterized by dynamic light scattering with non-invasive back scattering (DLS-NIBS), mixed mode measurements and phase analysis light scattering (M3-PALS) and high performance liquid chromatography with ultraviolet/visible light detection (HPLC-UV/VIS). Then, the efficacy of the obtained nanoformulations was examined via proof-of-principle experiments using in vitro cell assays. These assays showed an increment on cell motility and proliferation after treatment with free and encapsulated neuropeptides. Additionally, the effect of SP on wound healing was enhanced by the entrapment on CH-LP. Overall, the amenability of chitosan-based nanomaterials to encapsulate peptides and proteins constitutes a promising approach towards potential novel therapies to treat difficult wounds.

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Section: Resultscontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Chitosan—Based Liposome Formulation Enhances the In Vitro Wound Healing Efficacy of Substance P Neuropeptide

et al. 2017

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“…An important caveat, however, is that the anti-inflammatory function of SP cannot be universally ascribed to every cell type as this neuropeptide appears to have a pro-inflammatory effect in keratinocytes. 77 It is interesting to note that other neuropeptides such as calcitonin gene related peptide [78][79][80] may also be considered as an anti-inflammatory agent.…”

Section: Discussionmentioning

confidence: 99%

The neuropeptide Substance P facilitates the transition from an inflammatory to proliferation phase‐associated responses in dermal fibroblasts

Zaarour

Saha

Dey

et al. 2022

Experimental Dermatology

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The wound healing process is a product of three successive and overlapping phases of inflammation, proliferation and remodelling. Considerable efforts have been invested in deconstructing the intercellular crosstalk that orchestrates tissue repair, and we investigated the role of neuropeptides released from peripheral neurons upon injury in mediating these interactions. Amongst the most abundant of these neuropeptides secreted by nerves in the skin, is Substance P (SP). Given the role of dermal fibroblasts in coordinating multiple processes in the wound healing program, the effect of SP on human dermal fibroblasts of different ages was evaluated. The use of a substrate that recapitulates the mechanical properties of the in vivo tissue revealed novel effects of SP on dermal fibroblasts, including a block in inflammatory cytokine expression. Moreover, SP can promote expression of some extracellular matrix components and generates signals that regulate angiogenesis. Interestingly, the response of fibroblasts to SP was reduced concomitant with donor age. Altogether, SP acts to inhibit the inflammatory responses and promote proliferation‐associated responses in an age‐dependent manner in dermal fibroblasts, suggesting a role as a molecular switch between the inflammatory and proliferative phases of the wound healing response.

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Calcitonin gene-related peptide (CGRP): role in peripheral nerve regeneration

Chung

2017

Reviews in the Neurosciences

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Calcitonin gene-related peptide (CGRP) is a neuropeptide that has an important anti-inflammatory role in the immune system. Research has shown that CGRP is an integral part in peripheral nerve regeneration by (1) suppressing tumor necrosis factor-α, (2) forming an initial nerve bridge by increasing fibroblast motility and extracellular matrix synthesis, (3) vascularizing the spinal cord injury site, and (4) inducing Schwann cell (SC) proliferation. In this treatise, the following hypotheses will be explored: (1) CGRP is induced by c-Jun to regulate SC dedifferentiation, (2) CGRP promotes the chemotaxic migration of SCs along the nerve bridge, and (3) CGRP induces myelinophagy by activating various signaling pathways, such as p38 mitogen-activated protein kinase and Raf/extracellular signal-regulated kinase. These processes provide a framework for understanding the role of CGRP in peripheral nerve regeneration, which may be important in developing better strategies for nerve repair and gaining further insight into demyelinating diseases.

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The action of CGRP and SP on cultured skin fibroblasts

Abstract: Abstract

Cited by 3 publications

References 68 publications

A Chitosan—Based Liposome Formulation Enhances the In Vitro Wound Healing Efficacy of Substance P Neuropeptide

A Chitosan—Based Liposome Formulation Enhances the In Vitro Wound Healing Efficacy of Substance P Neuropeptide

The neuropeptide Substance P facilitates the transition from an inflammatory to proliferation phase‐associated responses in dermal fibroblasts

Calcitonin gene-related peptide (CGRP): role in peripheral nerve regeneration

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