Substrate influence on cell shape and cell mechanics: HepG2 cells spread on positively charged surfaces

Saravia, Verónica; Toca‐Herrera, José Luis

doi:10.1002/jemt.20742

Cited by 33 publications

(27 citation statements)

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“…Cell density, spreading, and shape have been previously shown to be influenced by cell-surface interactions [21,29,44,51]. In the context of this study, we demonstrated a similar influence, but also demonstrated a statistical correlation between these characteristics and the success of gene delivery using PEI.…”

Section: Discussionsupporting

confidence: 82%

The role of surface chemistry-induced cell characteristics on nonviral gene delivery to mouse fibroblasts

Kasputis

Pannier

2012

J Biol Eng

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BackgroundGene delivery approaches serve as a platform to modify gene expression of a cell population with applications including functional genomics, tissue engineering, and gene therapy. The delivery of exogenous genetic material via nonviral vectors has proven to be less toxic and to cause less of an immune response in comparison to viral vectors, but with decreased efficiency of gene transfer. Attempts have been made to improve nonviral gene transfer efficiency by modifying physicochemical properties of gene delivery vectors as well as developing new delivery techniques. In order to further improve and understand nonviral gene delivery, our approach focuses on the cell-material interface, since materials are known to modulate cell behavior, potentially rendering cells more responsive to nonviral gene transfer. In this study, self-assembled monolayers of alkanethiols on gold were employed as model biomaterial interfaces with varying surface chemistries. NIH/3T3 mouse fibroblasts were seeded on the modified surfaces and transfected using either lipid- or polymer- based complexing agents.ResultsTransfection was increased in cells on charged hydrophilic surfaces presenting carboxylic acid terminal functional groups, while cells on uncharged hydrophobic surfaces presenting methyl terminations demonstrated reduced transfection for both complexing agents. Surface–induced cellular characteristics that were hypothesized to affect nonviral gene transfer were subsequently investigated. Cells on charged hydrophilic surfaces presented higher cell densities, more cell spreading, more cells with ellipsoid morphologies, and increased quantities of focal adhesions and cytoskeleton features within cells, in contrast to cell on uncharged hydrophobic surfaces, and these cell behaviors were subsequently correlated to transfection characteristics.ConclusionsExtracellular influences on nonviral gene delivery were investigated by evaluating the upregulation and downregulation of transgene expression as a function of the cell behaviors induced by changes in the cells’ microenvronments. This study demonstrates that simple surface modifications can lead to changes in the efficiency of nonviral gene delivery. In addition, statistically significant differences in various surface-induced cell characteristics were statistically correlated to transfection trends in fibroblasts using both lipid and polymer mediated DNA delivery approaches. The correlations between the evaluated complexing agents and cell behaviors (cell density, spreading, shape, cytoskeleton, focal adhesions, and viability) suggest that polymer-mediated transfection is correlated to cell morphological traits while lipid-mediated transfection correlates to proliferative characteristics.

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Section: Discussionsupporting

confidence: 82%

The role of surface chemistry-induced cell characteristics on nonviral gene delivery to mouse fibroblasts

Kasputis

Pannier

2012

J Biol Eng

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“…Endothelial cell migration and attachment may be affected by various surface properties, including hydrophilicity, roughness, material, and charge distribution. [18][19][20][21] In serum containing media, proteins approach the surface much faster than cells. 8 Protein adsorption is stronger on hydrophilic than hydrophobic surfaces.…”

Section: Discussionmentioning

confidence: 99%

Increased affinity of endothelial cells to NiTi using ultraviolet irradiation: An in vitro study

Tateshima

Kaneko

Yamada

et al. 2017

J Biomedical Materials Res

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Nickel-titanium alloy (NiTi) is one of the most popular materials used endovascularly because of its shape memory and superelasticity. The NiTi device needs to be covered by endothelial cells after being placed in the blood vessel to reduce ischemic complications. The objective of this study was to examine the impact of ultraviolet (UV) irradiation on the biocompatibility of NiTi surfaces with endothelial cells. NiTi sheets were treated with UV irradiation for 48 h and human aorta derived endothelial cells were used in this study. UV irradiation converted the NiTi surface to hydrophilic state and increased albumin adsorption. The number of endothelial cell migration, attachment, proliferation as well as their metabolic activity were significantly increased on UV treated NiTi. This study provides the first evidence of the photoactivation of NiTi surfaces by UV irradiation and demonstrates improved biocompatibility of UV-treated NiTi surfaces with vascular endothelial cells. These results suggest that UV irradiation may promote endothelialization of NiTi devices in blood vessels. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1034-1038, 2018.

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“…Higher uptake seems to take place when the HepG2 cells have a larger Young modulus and bigger exposed area (Saravia and Toca‐Herrera, ); therefore, these two parameters are complementing each other.…”

Section: Discussionmentioning

confidence: 99%

Influence of HepG2 cell shape on nanoparticle uptake

Prats-Mateu

Ertl

Toca-Herrera

2014

Microscopy Res & Technique

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Cell mechanics provides insights in cell responses to external stress, which is an important parameter known to influence a variety of cell functions. Understanding the interdependence between mechanical stimulus, cell shape and function is essential in controlling cell culture microenvironment. In this paper, we report on the effect of cationic and anionic interfaces on cell shape and nanoparticle uptake activity of hepatocellular carcinoma cells HepG2. The shape of HepG2 cells changed from a round-like shape to a spread-like form exhibiting lamellar protrusions by incubating them on coated polystyrene well plates with polystyrene sulfonate and poly-ethylene imine (PEI), respectively. This change in shape of HepG2 cells did not influence the uptake of 49-nm particles (which entered the cells by diffusion). However, the internalization of 240-nm diameter particles was larger on cells seeded on cationic PEI. Particle uptake was measured at 4°C and 37°C; the optimal incubation time was 6 h. Cell shape and particle uptake were monitored by fluorescence and confocal microscopy. Quantification of particle internalization was carried out with flow cytometry.

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Substrate influence on cell shape and cell mechanics: HepG2 cells spread on positively charged surfaces

Cited by 33 publications

References 50 publications

The role of surface chemistry-induced cell characteristics on nonviral gene delivery to mouse fibroblasts

The role of surface chemistry-induced cell characteristics on nonviral gene delivery to mouse fibroblasts

Increased affinity of endothelial cells to NiTi using ultraviolet irradiation: An in vitro study

Influence of HepG2 cell shape on nanoparticle uptake

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