Substrate Modulation of the Activity of an Artificial Nanoesterase Made of Peptide‐Functionalized Gold Nanoparticles

Amphiphilic metalloaggregates: Catalysis, transport, and sensing

“…When a dodecapeptide, comprising a combination of a His, two Arg, and a Lys residue (among other amino acids), was grafted on Au-nanoparticles [49] we expected that their cooperativity could enable nucleophilic, general acid, and/or general base catalysis, but also stabilization of the negatively charged transition state that arises along the pathway of ester hydrolysis. Using the very same substrate of the previous example we observed that at low pH values the nanoparticle-based catalyst behaves very similarly to the previous dipeptide-based system, although the dodecapeptidenanoparticle has an additional 10-fold gain in activity.…”

An Artificial Supramolecular Nanozyme Based on β-Cyclodextrin-Modified Gold Nanoparticles

“…Recently, a special interest focuses on the construction of colloid nanoparticles for a variety of applications, for example, various organic and biological molecules such as sulfur-based compounds, proteins, DNA, enzymes [4][5][6] and cyclodextrins [7,8], have been used to modify nanoparticles for new functions. Among these nanomaterials, the construction of novel water-soluble nanocatalysts based on colloidal particles has received special interests for chemists and biologists [9][10][11][12][13].…”

Design of Artificial Selenoenzymes Based on Macromolecular Scaffolds

“…Tailoring the properties of nanostructure in a very predictable manner, one can form more complex nano‐architectures on surfaces and can establish the needs of specific applications. Thus, nanotechnology attracts scientists' great interest and it has been widely used in the design of molecular electronics, biosensors, medical diagnostics, coatings and drug delivery etc 70–75…”

“…The application of nano‐scale materials in constructing artificial enzymes has been widely reported 70–73. For mimicking the property of GPx, recently Haratake and Nakayama et al76 reported a nanosized glutathione peroxidase mimic, in which catalytic selenocysteine (SeCyst) was conjugated to a hydrophilic linear polysaccharide, pullulan (Pul) (Figure 6).…”