Substrate preference of Bifidobacterium adolescentis MB 239: compared growth on single and mixed carbohydrates

Amaretti, Alberto; Tamburini, Elena; Bernardi, Tatiana; Pompei, Anna; Zanoni, Simona; Vaccari, G.; Matteuzzi, Diego; Rossi, Maddalena

doi:10.1007/s00253-006-0500-9

Cited by 58 publications

(43 citation statements)

References 40 publications

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“…Galactose led to the highest growth rate and cellular yield, whereas glucose was the poorest carbon source for growth. GOS and lactose were both worse growth substrates than galactose but better than glucose, in agreement with a study demonstrating that B. adolescentis MB 239 preferred oligosaccharides over glucose (1).…”

Section: Discussionsupporting

confidence: 73%

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Kinetics and Metabolism of Bifidobacterium adolescentis MB 239 Growing on Glucose, Galactose, Lactose, and Galactooligosaccharides

Amaretti

Bernardi

Tamburini

et al. 2007

Appl Environ Microbiol

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102

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The kinetics and the metabolism of Bifidobacterium adolescentis MB 239 growing on galactooligosaccharides (GOS), lactose, galactose, and glucose were investigated. An unstructured unsegregated model for growth in batch cultures was developed, and kinetic parameters were calculated with a recursive algorithm. The growth rate and cellular yield were highest on galactose, followed by lactose and GOS, and were lowest on glucose. Lactate, acetate, and ethanol yields allowed the calculation of carbon fluxes toward fermentation products. Distributions between two-and three-carbon products were similar on all the carbohydrates (55 and 45%, respectively), but ethanol yields were different on glucose, GOS, lactose, and galactose, in decreasing order of production. Based on the stoichiometry of the fructose-6-phosphate shunt and on the carbon distribution among the products, the ATP yield was calculated. The highest yield was obtained on galactose, while the yields were 5, 8, and 25% lower on lactose, GOS, and glucose, respectively. Therefore, a correspondence among ethanol production, low ATP yields, and low biomass production was established, demonstrating that carbohydrate preferences may result from different distributions of carbon fluxes through the fermentative pathway. During the fermentation of a GOS mixture, substrate selectivity based on the degree of polymerization was exhibited, since lactose and the trisaccharide were the first to be consumed, while a delay was observed until longer oligosaccharides were utilized. Throughout the growth on both lactose and GOS, galactose accumulated in the cultural broth, suggesting that ␤(1-4) galactosides can be hydrolyzed before they are taken up.Many nondigestible oligosaccharides, such as fructooligosaccharides, isomaltooligosaccharides, galactooligosaccharides (GOS), and xylooligosaccharides, have been reported to beneficially affect human health. They are defined as prebiotics and are increasingly being used as functional food ingredients (18). ␤-GOS are manufactured from highly concentrated lactose solutions by the action of ␤-galactosidases (␤-Gals) which have transgalactosylation activity. In standardized large-scale production, trisaccharides to hexasaccharides are the main products; hence, commercial GOS occur as mixtures of various degrees of polymerization (DP) and glycosidic linkages and contain large amounts of glucose and unreacted lactose. The linkage between galactose moieties (mainly ␤1-4, ␤1-6, and ␤1-3), the efficiency of transgalactosylation, and the components in the final product depend upon the enzymes and the reaction conditions (3,7,25,30,34,39,41).As demonstrated in several in vitro and in vivo studies (5, 40), GOS resist hydrolysis by human digestive enzymes and are not absorbed on transit through the small intestine; therefore, they are available for fermentation by the colon-resident microflora. The utilization of GOS by the major intestinal bacteria has been investigated (25,34,46). It was shown that ␤1-4-linked oligosaccharides were selectivel...

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Section: Discussionsupporting

confidence: 73%

“…In fact, monosaccharides were preferred over oligosaccharides in a few cases (19,24,44), while growth rates and cell yields were higher on disaccharides and oligosaccharides than on their monomeric moieties in many others (1,9,12,13,22,26,43).…”

Section: Discussionmentioning

confidence: 99%

Kinetics and Metabolism of Bifidobacterium adolescentis MB 239 Growing on Glucose, Galactose, Lactose, and Galactooligosaccharides

Amaretti

Bernardi

Tamburini

et al. 2007

Appl Environ Microbiol

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102

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“…In this context, the Bifidobacterium adolescentis-specific growth rate was higher in lactose (Lac) than in glucose (Glc) (15,17), and Bifidobacterium longum NCC2705 preferentially uses Lac over Glc as a carbon source when grown in the presence of both sugars (14). The authors also found the glcP-ptsG locus to be involved in the transport of Glc into the cell, with glcP being downregulated by Lac (14).…”

mentioning

confidence: 88%

Catabolism of Glucose and Lactose in Bifidobacterium animalis subsp. lactis, Studied by ¹³ C Nuclear Magnetic Resonance

González-Rodríguez

Gaspar

Sánchez

et al. 2013

Appl Environ Microbiol

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“…Some, if not all, dominant intestinal anaerobes preferentially utilize oligosaccharides over mono-and disaccharides (2). Moreover, at least some minor members of the intestinal microbiota grow by cross-feeding on sugars released by the polysaccharide degraders (17,40).…”

Section: Discussionmentioning

confidence: 99%

Glycogen and Maltose Utilization by Escherichia coli O157:H7 in the Mouse Intestine

et al. 2008

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Mutant screens and transcriptome studies led us to consider whether the metabolism of glucose polymers, i.e., maltose, maltodextrin, and glycogen, is important for Escherichia coli colonization of the intestine. By using the streptomycin-treated mouse model, we found that catabolism of the disaccharide maltose provides a competitive advantage in vivo to pathogenic E. coli O157:H7 and commensal E. coli K-12, whereas degradation of exogenous forms of the more complex glucose polymer, maltodextrin, does not. The endogenous glucose polymer, glycogen, appears to play an important role in colonization, since mutants that are unable to synthesize or degrade glycogen have significant colonization defects. In support of the hypothesis that E. coli relies on internal carbon stores to maintain colonization during periods of famine, we found that by providing a constant supply of a readily metabolized sugar, i.e., gluconate, in the animal's drinking water, the competitive disadvantage of E. coli glycogen metabolism mutants is rescued. The results suggest that glycogen storage may be widespread in enteric bacteria because it is necessary for maintaining rapid growth in the intestine, where there is intense competition for resources and occasional famine. An important implication of this study is that the sugars used by E. coli are present in limited quantities in the intestine, making endogenous carbon stores valuable. Thus, there may be merit to combating enteric infections by using probiotics or prebiotics to manipulate the intestinal microbiota in such a way as to limit the availability of sugars preferred by E. coli O157:H7 and perhaps other pathogens.

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Substrate preference of Bifidobacterium adolescentis MB 239: compared growth on single and mixed carbohydrates

Cited by 58 publications

References 40 publications

Kinetics and Metabolism of Bifidobacterium adolescentis MB 239 Growing on Glucose, Galactose, Lactose, and Galactooligosaccharides

Kinetics and Metabolism of Bifidobacterium adolescentis MB 239 Growing on Glucose, Galactose, Lactose, and Galactooligosaccharides

Catabolism of Glucose and Lactose in Bifidobacterium animalis subsp. lactis, Studied by ¹³ C Nuclear Magnetic Resonance

Glycogen and Maltose Utilization by Escherichia coli O157:H7 in the Mouse Intestine

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Substrate preference of Bifidobacterium adolescentis MB 239: compared growth on single and mixed carbohydrates

Cited by 58 publications

References 40 publications

Kinetics and Metabolism of Bifidobacterium adolescentis MB 239 Growing on Glucose, Galactose, Lactose, and Galactooligosaccharides

Kinetics and Metabolism of Bifidobacterium adolescentis MB 239 Growing on Glucose, Galactose, Lactose, and Galactooligosaccharides

Catabolism of Glucose and Lactose in Bifidobacterium animalis subsp. lactis, Studied by 13 C Nuclear Magnetic Resonance

Glycogen and Maltose Utilization by Escherichia coli O157:H7 in the Mouse Intestine

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Catabolism of Glucose and Lactose in Bifidobacterium animalis subsp. lactis, Studied by ¹³ C Nuclear Magnetic Resonance