Substrate Specificity of Enzymes from Papaya Callus Cultures

Mell, Galen P.; Medora, Rustem S.; Bilderback, D. E.

doi:10.1016/s0044-328x(79)80101-4

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2003

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Cited by 4 publications

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“…Although numerous studies have dealt with the role of proteases in plants, only a few investigations have reported the protease levels in cultured cells [1,2,3,4], and none of these studies dealt with aging cultures. The purpose of this study was to determine the relationship between the protein content and protease activity present in cultured cells of increasing age.…”

Section: Introductionmentioning

confidence: 99%

Changes in protein and protease activity during the growth and senescence of Paul's Scarlet rose

Reider¹,

Fletcher

1986

Plant Cell Tiss Organ Cult

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A study was performed to determine the relationship between the protein content and protease activity in suspension cultures of rose (Rosa ev Paul's Scarlet) grown over a 30 day period. Protein levels and protease activity were calculated on a per culture and per cell basis. Older nongrowing 14 day-old cultures possessed the largest total protease activity, but the highest concentration of protease activity per cell was in young 4 day-old rapidly dividing ceils.

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Section: Introductionmentioning

confidence: 99%

Changes in protein and protease activity during the growth and senescence of Paul's Scarlet rose

Reider¹,

Fletcher

1986

Plant Cell Tiss Organ Cult

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Carica papaya

Ross¹

2003

Medicinal Plants of the World

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Enzymatic difference between laticifers and cultured cells of papaya

et al. 1986

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Cell suspension cultures of Carica papaya were found to produce a papain-like enzyme showing an amidase activity similar to papain. Experiments suggested that the enzyme was an SH-enzyme, but an immunological test indicated the absence of papain in cultured cells. The isoelectric point (4.3) of the enzyme of cultured cells was the same as that of the leaf extract, but was different from that of papain or other amidases in the latex of the fruit.

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Substrate Specificity of Enzymes from Papaya Callus Cultures

Cited by 4 publications

References 3 publications

Changes in protein and protease activity during the growth and senescence of Paul's Scarlet rose

Changes in protein and protease activity during the growth and senescence of Paul's Scarlet rose

Carica papaya

Enzymatic difference between laticifers and cultured cells of papaya

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