Substrate stiffness does affect the fate of human keratinocytes

Gupta, Prerak; Narayana, Sree; Kasiviswanathan, Uvanesh; Agarwal, Tarun; Senthilguru, K.; Mukhopadhyay, Devdeep; Pal, Kunal; Giri, Supratim; Maiti, Tapas K.; Banerjee, Indranil

doi:10.1039/c5ra19947f

Cited by 28 publications

(19 citation statements)

References 46 publications

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“…On the other hand, when cultured on a slightly stiffer matrix (10 kPa and 20 kPa) cells showed survival and showed higher proliferation as compared to TCP (Fig. 1) which has also been shown with PDMS 30. This concludes that stiffness not only plays a critical role in regulating the proliferation but is also involved in cell survival.…”

Section: Discussionsupporting

confidence: 59%

Hydrogel scaffold with substrate elasticity mimicking physiological-niche promotes proliferation of functional keratinocytes

et al. 2019

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Section: Discussionsupporting

confidence: 59%

Hydrogel scaffold with substrate elasticity mimicking physiological-niche promotes proliferation of functional keratinocytes

et al. 2019

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“…Analysis at the nuclear level, showed that the soft surface caused nuclear mechanotransduction (evident from nuclear lamin A/C expression) and perturbed the transcription pattern of protein (VEGF as model protein) in HaCaT cells. This study confirmed that the human keratinocytes are mechanosensitive toward substrate 55 .…”

Section: Biological Performance Of Electrospun Matssupporting

confidence: 83%

Modulating matrix‐multicellular response using polysucrose‐blended with poly‐L‐lactide or polydioxanone in electrospun scaffolds for skin tissue regeneration

Chummun

Bhaw‐Luximon

Jhurry

2018

J Biomedical Materials Res

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Polysucrose (PSuc) is hydrophilic, has excellent biocompatibility with cells as a density gradient and is resistant to enzymes. Its use in electrospun mats for tissue engineering applications has not been investigated due to its amorphous nature. For spinnability and robustness, polysucrose was blended with poly‐L‐lactide (PLLA) and polydioxanone (PDX) respectively and electrospun into nanofibrous mats. Interaction with cells was assessed using L929 mouse fibroblasts and HaCaT keratinocytes separately and in co‐culture. Effect of parameters such as porosity, fiber diameter, surface wettability and mechanical properties of mats on cell‐scaffold interactions was studied. Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber‐like projections of 100 nm and 200–300 nm in diameter respectively from the periphery or center of cells. Granular extracellular matrix was formed on both PLLA‐PSuc and PDX‐PSuc 50–50 seeded with keratinocytes. Growth of keratinocytes was enhanced in co‐culture with fibroblasts with the formation of a skin‐like layer. Both cells showed the ability to form multilayer structures. The mats maintained their physical integrity during the period of study. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3275–3291, 2018.

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“…On the other hand, the cells grown on the 1 µm CL materials were about as active as those grown on the 1 µm µCP materials. In a recent study by Gupta et al [58], it was reported that keratinocyte proliferation increased with an increased PDMS surface stiffness (0.05–1.6 MPa) [58]. This could be an explanation as to why some CL surfaces, which were stiffer, had >150% of dye reduction, while the dye reduction of all the softer µCP surfaces was <150%.…”

Section: Discussionmentioning

confidence: 99%

Bifunctional Bioactive Polymer Surfaces with Micrometer and Submicrometer-sized Structure: The Effects of Structure Spacing and Elastic Modulus on Bioactivity

Elsayed

Widyaya²,

Shafi³

et al. 2019

Molecules

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This study presents a comparison of two types of bifunctional structured surface that were made from the same polymer –– an antimicrobial polycation (a synthetic mimic of an antimicrobial peptide, SMAMP) and a protein-repellent polyzwitterion (poly(sulfobetaines), PSB). The first type of bifunctional surface was fabricated by a colloidal lithography (CL) based process where the two polymers were immobilized sequentially onto pre-structured surfaces with a chemical contrast (gold on silicon). This enabled site-selective covalent attachment. The CL materials had a spacing ranging from 200 nm to 2 µm. The second type of structured surface (spacing: 1 – 8.5 µm) was fabricated using a microcontact printing (µCP) process where SMAMP patches were printed onto a PSB network, so that 3D surface features were obtained. The thus obtained materials were studied by quantitative nanomechanical measurements using atomic force microscopy (QNM-AFM). The different architectures led to different local elastic moduli at the polymer-air interface, where the CL surfaces were much stiffer (Derjaguin-Muller-Toporov (DMT) modulus = 20 ± 0.8 GPa) compared to the structured 3D networks obtained by µCP (DMT modulus = 42 ± 1.1 MPa). The effects of the surface topology and stiffness on the antimicrobial activity against Escherichia coli, the protein repellency (using fibrinogen), and the compatibility with human gingival mucosal keratinocytes were investigated. The softer 3D µCP surfaces had simultaneous antimicrobial activity, protein repellency, and cell compatibility at all spacings. For the stiffer CL surfaces, quantitative simultaneous antimicrobial activity and protein repellency was not obtained. However, the cell compatibility could be maintained at all spacings. The optimum spacing for the CL materials was in the range of 500 nm–1 µm, with significantly reduced antimicrobial activity at 2 µm spacing. Thus, the soft polymer network obtained by µCP could be more easily optimized than the stiff CL surface, and had a broader topology range of optimal or near-optimal bioactivity.

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Substrate stiffness does affect the fate of human keratinocytes

Abstract: Epithelial cells response to the varying stiffness of polydimethyl siloxane (PDMS) substrate.

Cited by 28 publications

References 46 publications

Hydrogel scaffold with substrate elasticity mimicking physiological-niche promotes proliferation of functional keratinocytes

Hydrogel scaffold with substrate elasticity mimicking physiological-niche promotes proliferation of functional keratinocytes

Modulating matrix‐multicellular response using polysucrose‐blended with poly‐L‐lactide or polydioxanone in electrospun scaffolds for skin tissue regeneration

Bifunctional Bioactive Polymer Surfaces with Micrometer and Submicrometer-sized Structure: The Effects of Structure Spacing and Elastic Modulus on Bioactivity

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