Substrate viscoelasticity affects human macrophage morphology and phagocytosis

Kalashnikov, Nikita; Moraes, Christopher

doi:10.1039/d2sm01683d

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Effects Of Biomaterials Properties On the Immune Response Of...1

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2024

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Cited by 7 publications

(1 citation statement)

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“…A tunable viscoelastic polyacrylamide (vPAA) hydrogel culture system was developed to investigate the impact of viscoelasticity on macrophage behavior. 95 THP-1 macrophages were seeded onto three vPAA hydrogels with similar storage moduli (5.7 kPa) but varying loss moduli (low: 11.6 ± 5.5 Pa, medium: 191.6 ± 7.8 Pa, high: 356.7 ± 21.37 Pa). Subsequently, the macrophage morphology and phagocytosis were observed and evaluated.…”

Section: Effects Of Biomaterials Properties On the Immune Response Of...mentioning

confidence: 99%

Potential effects of biomaterials on macrophage function and their signalling pathways

Zhu,

Wang,

Zhu

et al. 2023

Biomater. Sci.

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Section: Effects Of Biomaterials Properties On the Immune Response Of...mentioning

confidence: 99%

Potential effects of biomaterials on macrophage function and their signalling pathways

Zhu,

Wang,

Zhu

et al. 2023

Biomater. Sci.

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Understanding Macrophage‐Tumor Interactions: Insights from Single‐Cell Behavior Monitoring in a Sessile Microdroplet System

Lin,

Zhang,

Xie

et al. 2024

Small Methods

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Interaction between tumor‐associated macrophages and tumor cells is crucial for tumor development, metastasis, and the related immune process. However, the macrophages are highly heterogeneous spanning from anti‐tumorigenic to pro‐tumorigenic, which needs to be understood at the single‐cell level. Herein, a sessile microdroplet system designed for monitoring cellular behavior and analyzing intercellular interaction, demonstrated with macrophage‐tumor cell pairs is presented. An automatic procedure based on the inkjet printing method is utilized for the precise pairing and co‐encapsulation of heterotypic cells within picoliter droplets. The sessile nature of microdroplets ensures controlled fusion and provides stable environments conducive to adherent cell culture. The nitric oxide generation and morphological changes over incubation are explored to reveal the complicated interactions from a single‐cell perspective. The immune response of macrophages under distinct cellular microenvironments is recorded. The results demonstrate that the tumor microenvironment displays a modulating role in polarizing macrophages from anti‐tumorigenic into pro‐tumorigenic phenotype. The approach provides a versatile and compatible platform to investigate intercellular interaction at the single‐cell level, showing promising potential for advancing single‐cell behavior studies.

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The Molecular Mechanism of Macrophages in Response to Mechanical Stress

Su,

Yin

2024

Ann Biomed Eng

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Substrate viscoelasticity affects human macrophage morphology and phagocytosis

Abstract: Viscoelasticity is an inherent characteristic of many living tissues and, in an attempt to better recapitulate this aspect in cell culture, hydrogel biomaterials have been engineered to exhibit time-dependent energy-dissipative...

Cited by 7 publications

References 46 publications

Potential effects of biomaterials on macrophage function and their signalling pathways

Potential effects of biomaterials on macrophage function and their signalling pathways

Understanding Macrophage‐Tumor Interactions: Insights from Single‐Cell Behavior Monitoring in a Sessile Microdroplet System

The Molecular Mechanism of Macrophages in Response to Mechanical Stress

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