Substrates and Surfaces for Control of Pluripotent Stem Cell Fate and Function

Srinivasan, Akshaya; Toh, Yi‐Chin; Loh, Xian Jun; Toh, Wei Seong

doi:10.1002/9781119242642.ch12

Cited by 1 publication

(2 citation statements)

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“…The other conventional methods of culturing PSCs involve using ECM components with cell culture media supplemented with growth factors that regulate genes related to pluripotency (Srinivasan et al, 2016) -either to up-regulate promoters of pluripotency or down-regulate inhibitors of pluripotency. The growth factors used depend on the pathways to be regulated, which depend on the cell type.…”

Section: Conventional Methods Of Psc Expansionmentioning

confidence: 99%

“…Therefore, it is worth exploring the use of physical and mechanical cues in PSC maintenance and expansion, which could improve the large-scale xeno-free expansion. We recommend a book chapter (Srinivasan et al, 2016) for a comprehensive review of conventional hPSC expansion, a review by Dakhore et al (2018) that compares hPSC expansion media, and a review by Hayashi and Furue (2016) that summarizes substrates used in hPSC expansion.…”

Section: Conventional Methods Of Psc Expansionmentioning

confidence: 99%

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Emerging Methods for Enhancing Pluripotent Stem Cell Expansion

Chan

Rizwan

Yim

2020

Front. Cell Dev. Biol.

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Pluripotent stem cells (PSCs) have great potential to revolutionize the fields of tissue engineering and regenerative medicine as well as stem cell therapeutics. However, the end goal of using PSCs for therapeutic use remains distant due to limitations in current PSC production. Conventional methods for PSC expansion have limited potential to be scaled up to produce the number of cells required for the end-goal of therapeutic use due to xenogenic components, high cost or low efficiency. In this mini review, we explore novel methods and emerging technologies of improving PSC expansion: the use of the two-dimensional mechanobiological strategies of topography and stiffness and the use of three-dimensional (3D) expansion methods including encapsulation, microcarrierbased culture, and suspension culture. Additionally, we discuss the limitations of conventional PSC expansion methods as well as the challenges in implementing non-conventional methods.

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Section: Conventional Methods Of Psc Expansionmentioning

confidence: 99%