Substrates for storing entomopathogenic nematodes (Rhabditida: Steinernematidae, Heterorhabditidae)

Andaló, Vanessa; Cavalcanti, Ricardo Sousa; Molina, Juan Pablo Edwards; Moino, Alcides

doi:10.1590/s0103-90162010000300013

Cited by 10 publications

(4 citation statements)

References 21 publications

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“…When stored in aqueous solution, H. indica has been reported to settle down and form precipitate on the bottom of the container ( Askary et al, 2018 ). Agglomeration leaves the nematodes an environment of low oxygen content and loss of energy reserves which could be a factor for a shorter storage life of native isolate of H. indica ( Andaló et al, 2010 ). A comparison between the different storage temperatures over time reveals that IJ of native isolates H. indica survive better when stored at 15°C, than at any other temperature.…”

Section: Discussionmentioning

confidence: 99%

Ecological Characterisation of Native Isolates of Heterorhabditis indica from Viti Levu, Fiji Islands

Kour

Khurma

Brodie

2021

Journal of Nematology

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Entomopathogenic nematodes (EPNs) in the families Steinernematidae and Heterorhabditidae are obligate parasites of soil inhibiting insects. EPNs are being widely researched as promising biocontrol agents for a wide range of agricultural pests. It is known that strains of EPNs isolated from different geographical regions differ in their attributes, such as host-finding ability, host range, infectivity, reproduction, and environmental stress tolerance. A precise knowledge of these factors is therefore an essential pre-requisite for devising successful strategies to use these nematodes in biological control programmes. Thus, ecological characterisation of the EPN Heterorhabditis indica (Rhabditida: Heterorhabditidae) newly isolated and representing the only species of EPN reported from the island of Viti Levu, Fiji was carried out using Galleria mellonella larvae (L) (Pyralidae: Galleriinae) as hosts to allow comparisons between bioassays conducted in different laboratories around the world. Temperature data showed that native isolates of H. indica are warm-adapted nematodes with thermal range for infectivity between 15˚C and 35˚C and can reproduce between 20˚C and 30˚C. They are highly virulent with LC50 values against G. mellonella ranging from 2.8 IJ to 3.8 IJ/larva. However, they showed poor desiccation tolerance and fail to infect hosts in soil with moisture levels below 8%. They showed a moderate level of hypoxic tolerance and can be stored at 15˚C for 4 months. Results also showed great variability within the selected native isolates of H. indica. Beneficial traits for selected isolates were added up to identify a superior candidate. The current study also suggested that the thermal niche breadth for infection can differ among conspecific strains of an EPN species. The results of this experimental study on ecological aspects of these native isolates of H. indica should form a basis for their potential use in biological control of insect pests in Fiji.

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Section: Discussionmentioning

confidence: 99%

Ecological Characterisation of Native Isolates of Heterorhabditis indica from Viti Levu, Fiji Islands

Kour

Khurma

Brodie

2021

Journal of Nematology

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“…Infective juvenile survival may be extended by the addition of certain products to IJ suspensions, such as glycerin, which help keep the S. carpocapsae IJs alive and infective for longer periods when compared to water [81] . In addition, some substrates may assist and favor the storage of nematodes, such as the sponge that preserves H. amazonensis JPM4 IJs at 16 °C, with 50% viability after 6 months of storage [82] . An agar-based medium was also tested for the transport and storage of IJs.…”

Section: Formulationmentioning

confidence: 99%

Studies on entomopathogenic nematodes in Brazil: past and future

Dolinski¹,

Monteiro²,

Andaló³

et al. 2014

Nematoda

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HIGHLIGHTS• Lauro Travassos in 1927 created genus Steinernema.• Two new species of entomopathogenic nematodes were described.• Studies on ecology, biology, production, formulation and application of native species have been carried out since 2000. ABSTRACT:In Brazil, the first study with entomopathogenic nematodes (EPNs) was conducted by Lauro Travassos in 1927, when he created the genus Steineria, that latter was changed to Steinernema. After important meetings and courses, there was a great development of EPNs research in various states by different research groups in Brazil. Many EPN species were isolated throughout the country and two new species described: Heterorhabditis amazonensis and Steinernema brazilense. The behavioral aspects, life cycle and pathogenicity of native EPNs were analyzed. Also their persistence and survival in the soil and the biotic and abiotic factors that may affect their efficiency as biocontrol agents. Due to the particular native species characteristics, production, formulation and application technologies were developed for them. Here in we summarize main Brazilians studies in different areas using EPNs. This is the first review on the subject in Brazil.Keywords: biology, ecology, behavior, production, formulation.Cite as Dolinski C, Monteiro C, Andaló V, Leite LG. Studies on entomopathogenic nematodes in Brazil: past and future. Nematoda. 2017;4:e102017. http://dx

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“…Many studies proved the effectiveness of EPNs against a variety of agricultural pests in both laboratory and field conditions (Ansari et al, 2006;Susurluk et al, 2009;Kepenekci et al, 2016;Odendaal et al, 2016;Mokrini et al, 2020). However, the effectiveness of EPNs is closely linked to their ability to infect and kill pests, which is influenced by a variety of factors, including pre-application storage conditions (Grewal, 2000;Andaló et al, 2010Andaló et al, , 2011. Differences in storage conditions and durations lead to a reduction in the quality of the infective juveniles (IJs) of different EPN species depending on the different storage factors such as oxygen and optimal temperature requirements of species (Qiu and Bedding, 2000).…”

Section: Introductionmentioning

confidence: 99%

The Role of Storage Duration and Conditions on the Survival and Pathogenicity of Entomopathogenic Nematodes

AKI,

YÜKSEL,

İMREN

et al. 2023

Uluslararası Tarım Ve Yaban Hayatı Bilimleri Dergisi

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Entomopathogenic nematodes (EPNs) (Rhabditida: Heterorhabditidae and Steinernematidae) are effective biocontrol agents for many insect pests and are generally stored for a period of time prior to their use in the laboratory or field. However, unfavorable storage conditions have a great impact on the survival and infectivity of EPNs. This study was conducted to determine the optimum storage conditions of infective juveniles (IJs) of four native EPN species (Heterorhabditis bacteriophora FLH-4H, H. indica 216-H, Steinernema feltiae KCS-S, and S. bicornotum MGZ-4S) under laboratory conditions. The survival capability of the IJs was tested at different concentrations (500, 1000, 1500, and 2000 IJs), temperatures (9 and 25 °C) and storage media [double-distilled water (ddH2O), tap water, and sterile Ringer solution]. In general, the survival of IJs of tested EPN species was the highest at the 1st month after treatment (MAT) at the concentrations of 1000 and 1500 IJs and gradually decreased with the increasing storage periods. The survival rates of the IJs of Steirnematids were generally higher than Heterorhabditid species. The highest survival of IJs was generally obtained after 1-month storage in Ringer solution at 9°C while tap water led to poor survival for the IJs at both temperatures tested. The IJs that were stored at 9°C induced higher mortalities on the larvae of Galleria mellonella (L.) (Lepidoptera: Pyralidae). The results showed that the IJs of tested EPN species can remain viable for a longer period of time in Ringer solution at 1000 and 1500 IJs concentrations at 9°C without losing much of their infectivity.

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Substrates for storing entomopathogenic nematodes (Rhabditida: Steinernematidae, Heterorhabditidae)

Cited by 10 publications

References 21 publications

Ecological Characterisation of Native Isolates of Heterorhabditis indica from Viti Levu, Fiji Islands

Ecological Characterisation of Native Isolates of Heterorhabditis indica from Viti Levu, Fiji Islands

Studies on entomopathogenic nematodes in Brazil: past and future

The Role of Storage Duration and Conditions on the Survival and Pathogenicity of Entomopathogenic Nematodes

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