Substrates of Kinin-releasing Enzymes isolated from Horse Plasma

Kininogens of Human Plasma

“…Several investigators isolated LMW-kininogen from bovine plasma [19, 27-29, 36, 37, 64, 65] and a substrate with similar properties was isolated from horse plasma [31] and from rabbit plasma [8,16,17,50]. The preparation obtained by Habermann [29] was homogeneous by analytical ultracentrifugation with an S 20,w of 3.85 and a molecular weight of 48,000.…”

“…Omitting an earlier used acidification step, Henriques et al [31] succeeded in isolating HMW-kininogen from horse plasma by DEAE-cellulose chromatography. As described above, Habal et al [21] have shown that HMW-kininogen elutes with Cl-inactivator.…”

“…Several enzymes (trypsin, plasmin, pancreatic kallikrein and snake venom kininogenase) released kinin from LMW-kininogen of bovine or horse plasma origin, but plasma kallikrein cleaved only the HMW-kininogen, releasing no kinin from the LMW-kininogen derived from horse plasma [31], and only small quantities from that derived from bovine plasma [78].…”