Subtypes of non-transformed human mammary epithelial cells cultured in vitro: histo-blood group antigen H type 2 defines basal cell-derived cells

Karsten, Uwe; Papsdorf, G; Pauly, A; Vojtěšek, Bořivoj; Moll, Roland; Lane, E. Birgitte; Clausen, Henrik; Stosiek, P; Kasper, Michael

doi:10.1111/j.1432-0436.1993.tb00659.x

Cited by 17 publications

(9 citation statements)

References 64 publications

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“…In the present study, we noted that individual MUC-1 + /CALLA -/ESA + HBEC progenitors gave rise to colonies that varied markedly in their content of cells expressing one or more luminal markers. Similar observations of HBEC clonal heterogeneity have recently been reported by others [22]. Such clonal heterogeneity may be indicative of an underlying stochastic mechanism regulating HBEC differentiation independent of the presence of factors (e.g., EGF and INS) that may be required to support the viability and/or stimulate the proliferation of these cells.…”

Section: Discussionsupporting

confidence: 86%

Phenotypic and functional characterization in vitro of a multipotent epithelial cell present in the normal adult human breast

et al. 1998

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Section: Discussionsupporting

confidence: 86%

Phenotypic and functional characterization in vitro of a multipotent epithelial cell present in the normal adult human breast

et al. 1998

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“…Furthermore, they suggested that the MUC-1 + /CALLA - /ESA + and the MUC-1 -to ± /CALLA ± to + / ESA + progenitors are candidate in vivo alveolar and ductal progenitors, respectively [4]. HBEC clonal heterogeneity has also been reported by others [5]. Such clonal heterogeneity might be indicative of an underlying stochastic mechanism regulating HBEC differentiation independently of the presence of factors (such as epidermal growth factor and insulin) that might be required to support the viability and/or stimulate the proliferation of these cells [4].…”

Section: Introductionmentioning

confidence: 87%

Mammary stem cells, self-renewal pathways, and carcinogenesis

2005

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Bmi-1 = B lymphoma Mo-MLV insertion region 1; Dsh = Dishevelled; ESA = epithelial-specific antigen; Fu = Fused; GSK = glycogen synthase kinase; HBEC = human breast epithelial cell; Ihh = indian hedgehog; Ptch = patched; Shh = sonic hedgehog; Smo = smoothened; SuFu = suppressor of fused. Breast Cancer Research May 2005 Vol 7 No 3 Liu et al. AbstractThe mammary gland epithelial components are thought to arise from stem cells that undergo both self-renewal and differentiation. Self-renewal has been shown to be regulated by the Hedgehog, Notch, and Wnt pathways and the transcription factor B lymphoma Mo-MLV insertion region 1 (Bmi-1). We review data about the existence of stem cells in the mammary gland and the pathways regulating the self-renewal of these cells. We present evidence that deregulation of the self-renewal in stem cells/progenitors might be a key event in mammary carcinogenesis. If 'tumor stem cells' are inherently resistant to current therapies, targeting stem cell self-renewal pathways might provide a novel approach for breast cancer treatment.

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“…1(B)), the cells at the periphery appear more tightly arranged than those located at the center of the colony. Most of the cells within these colonies express MUC1, K8/18, EpCAM and K19, and do not express K14, CD44v6 and histo blood group antigen H type 2 (BGA2; 28,54,55). It is interesting to note that K19 is consistently expressed in these colonies (28,49,54), even though K19 is not a reliable marker of luminal epithelial cells in vivo (16).…”

Section: Characterization Of Hmec-derived Coloniesmentioning

confidence: 99%

“…Most of the cells within these colonies express MUC1, K8/18, EpCAM and K19, and do not express K14, CD44v6 and histo blood group antigen H type 2 (BGA2; 28,54,55). It is interesting to note that K19 is consistently expressed in these colonies (28,49,54), even though K19 is not a reliable marker of luminal epithelial cells in vivo (16). Cells having a luminal phenotype can be induced to further differentiate into casein-producing cells by the addition of fresh culture medium supplemented with of 1 µg/mL ovine prolactin and 50% Matrigel (30).…”

Section: Characterization Of Hmec-derived Coloniesmentioning

confidence: 99%

Epithelial Progenitors in the Normal Human Mammary Gland

Stingl

Raouf

Emerman

et al. 2005

J Mammary Gland Biol Neoplasia

146

122

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The human mammary gland is organized developmentally as a hierarchy of progenitor cells that become progressively restricted in their proliferative abilities and lineage options. Three types of human mammary epithelial cell progenitors are now identified. The first is thought to be a luminal-restricted progenitor; in vitro under conditions that support both luminal and myoepithelial cell differentiation, this cell produces clones of differentiating daughter cells that are exclusively positive for markers characteristic of luminal cells produced in vivo (i.e., keratins 8/18 and 19, epithelial cell adhesion molecule [EpCAM] and MUC1). The second type is a bipotent progenitor. It is identified by its ability to produce "mixed" colonies in single cell assays. These colonies contain a central core of cells expressing luminal markers surrounded by cells with a morphology and markers (e.g., keratin 14(+)) characteristic of myoepithelial cells. Serial passage in vitro of an enriched population of bipotent progenitors promotes the expansion of a third type of progenitor that is thought to be myoepithelial-restricted because it only produces cells with myoepithelial features. Luminal-restricted and bipotent progenitors can prospectively be isolated as distinct subpopulations from freshly dissociated suspensions of normal human mammary cells. Both are distinguished from many other cell types in mammary tissue by their expression of EpCAM and CD49f (alpha6 integrin). They are distinguished from each other by their differential expression of MUC1, which is expressed at much higher levels on the luminal progenitors. To relate the role of these progenitors to the generation of the three-dimensional tubuloalveolar structure of the mammary tree produced in vivo, we propose a model in which the commitment to the luminal versus the myoepithelial lineage may play a determining role in the generation of alveoli and ducts.

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Subtypes of non-transformed human mammary epithelial cells cultured in vitro: histo-blood group antigen H type 2 defines basal cell-derived cells

Cited by 17 publications

References 64 publications

Phenotypic and functional characterization in vitro of a multipotent epithelial cell present in the normal adult human breast

Phenotypic and functional characterization in vitro of a multipotent epithelial cell present in the normal adult human breast

Mammary stem cells, self-renewal pathways, and carcinogenesis

Epithelial Progenitors in the Normal Human Mammary Gland

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