1969
DOI: 10.1111/j.1432-1033.1969.tb00488.x
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Subunit Interactions of Glucose-6-Phosphate Dehydrogenase from Human Erythrocytes

Abstract: Glucose-6-phosphate dehydrogenase from human erythrocytes shows successive aggregationdissociation equilibria. The effects of salt concentration and pH show that a molecule of 210,000 molecular weight and ~2 0 ,~ of 9.0 S dissociated to a half-molecule of 105,000 molecular weight and S Z O ,~ of 5.6 S. Evidence for this being a discrete dissociation was provided by molecular weight determinations, relation between sedimentation coefficients and molecular weights, and the occurrence of skew patterns a t a parti… Show more

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Cited by 117 publications
(68 citation statements)
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“…The content of G6PD CRM was lower than estimated by the double-antibody radiommunoassay (8), probably because of the higher background of that procedure with respect to the solid-phase radioimmunoassay. The resulting specific activity in the eight normal subjects reveals a decided similarity to values observed for homogeneous preparations of the B-type enzyme (11,(23)(24)(25)(26).…”
Section: Resultsmentioning
confidence: 60%
“…The content of G6PD CRM was lower than estimated by the double-antibody radiommunoassay (8), probably because of the higher background of that procedure with respect to the solid-phase radioimmunoassay. The resulting specific activity in the eight normal subjects reveals a decided similarity to values observed for homogeneous preparations of the B-type enzyme (11,(23)(24)(25)(26).…”
Section: Resultsmentioning
confidence: 60%
“…Further purification of glucose-6-phosphate dehydrogenase was achieved using DEAE-Sephadex a t pH 6.5 and CM-Sephadex a t pH 5.8 in phosphate, with the buffer conditions as previously described [ 5 ] . These steps were carried out in a single day using 12.5-cm Buchner funnels for the separation.…”
Section: Further Purification Of Glucose-6-phosphate Dehydrogenase Amentioning
confidence: 99%
“…The procedure is described according to the steps involved with reference to 6-phosphogluconate dehydrogenase. Glucose-6-phosphate dehydrogenase was separated from 6-phosphogluconate dehydrogenase in step 4 and purified further according to the procedure of Cohen and Rosemeyer [5].…”
Section: Buffers Used In the Purification Proceduresmentioning
confidence: 99%
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