Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge

Musoke, A.J.; Rowlands, John; Nene, Vishvanath; Nyanjui, John K.; Katende, J.M.; Spooner, P.R.; Mwaura, Stephen; Odongo, David; Nkonge, Catherine; Mbogo, S.K.; Bishop, Richard P.; Morzaria, S.P.

doi:10.1016/j.vaccine.2004.09.039

Cited by 50 publications

(50 citation statements)

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“…Immunization with recombinant p67 provided cattle with immunity to homologous challenge (9). This was confirmed in field studies (10), although the observed 50% reduction in disease severity suggests that improvements are required before a p67-based vaccine can be considered an effective control measure. Recent studies on a T. parva isolate from Zambia (T. parva Chitongo) with low virulence and a reduced capacity to infect bovine lymphocytes (11) suggested that a second adhesin might exist.…”

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confidence: 70%

Role of the Polymorphic Immunodominant Molecule in Entry of Theileria parva Sporozoites into Bovine Lymphocytes

2014

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Theileria parva is a tick-transmitted apicomplexan parasite that infects cattle and African buffalo. In cattle, it causes a fatal lymphoproliferative disease called East Coast fever. The polymorphic immunodominant molecule (PIM) is expressed by two stages of the parasite: the sporozoite, which is inoculated by the tick to infect mammalian lymphocytes, and the schizont, the established intralymphocytic stage. Here, we demonstrate that monoclonal antibodies (MAb) to PIM can reduce the ability of sporozoites to infect bovine lymphocytes in vitro. This reduction appears to be due to blocking of sporozoite attachment by binding of the MAb to several regions of PIM. Interestingly, one MAb, which recognizes an epitope in the central variable region of PIM, did not inhibit sporozoite infectivity. We also demonstrate that PIM antigen, as a recombinant molecule, can also reduce sporozoite infectivity in vitro by blocking both attachment and internalization of sporozoites. Electron microscopic studies showed that PIM is present in microspheres below the sporozoite surface and is transported to the parasite surface soon after contact with bovine lymphocytes. The results suggest that at least two sporozoite molecules, PIM and the previously described p67, are involved in the entry of T. parva into mammalian lymphocytes.

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Role of the Polymorphic Immunodominant Molecule in Entry of Theileria parva Sporozoites into Bovine Lymphocytes

2014

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“…In three geographically separate trial sites, two showed no significant reduction of severe ECF. In the third site, a significant reduction of severe ECF by 30 % was observed [56]. Needle challenge experiments run concurrently with field trials showed a decrease in the incidents of severe ECF by approximately 50 % suggesting that protective efficacy varies between natural tick challenge and artificial needle challenge [56].…”

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confidence: 99%

Induction of humoral immune response to multiple recombinant Rhipicephalus appendiculatus antigens and their effect on tick feeding success and pathogen transmission

et al. 2016

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Background: Rhipicephalus appendiculatus is the primary vector of Theileria parva, the etiological agent of East Coast fever (ECF), a devastating disease of cattle in sub-Saharan Africa. We hypothesized that a vaccine targeting tick proteins that are involved in attachment and feeding might affect feeding success and possibly reduce tickborne transmission of T. parva. Here we report the evaluation of a multivalent vaccine cocktail of tick antigens for their ability to reduce R. appendiculatus feeding success and possibly reduce tick-transmission of T. parva in a natural host-tick-parasite challenge model. Methods: Cattle were inoculated with a multivalent antigen cocktail containing recombinant tick protective antigen subolesin as well as two additional R. appendiculatus saliva antigens: the cement protein TRP64, and three different histamine binding proteins. The cocktail also contained the T. parva sporozoite antigen p67C. The effect of vaccination on the feeding success of nymphal and adult R. appendiculatus ticks was evaluated together with the effect on transmission of T. parva using a tick challenge model.

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“…Primers designed from the p104 and p67 genes have been successfully used for PCR amplification and detection of T. parva infections (Skilton et al, 2002;Musoke et al, 2005). Prior to this study several LAMP primer sets were designed from the p67 and p104 genes of T. parva.…”

Section: Discussionmentioning

confidence: 99%

Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes

Thekisoe

Rambritch

Nakao

et al. 2010

International Journal for Parasitology

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Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge

Cited by 50 publications

References 31 publications

Role of the Polymorphic Immunodominant Molecule in Entry of Theileria parva Sporozoites into Bovine Lymphocytes

Role of the Polymorphic Immunodominant Molecule in Entry of Theileria parva Sporozoites into Bovine Lymphocytes

Induction of humoral immune response to multiple recombinant Rhipicephalus appendiculatus antigens and their effect on tick feeding success and pathogen transmission

Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes

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