Successful artificial insemination using semen frozen and stored by an ultrafreezer in the Majorera goat breed

Batista, M.; Niño, T; Alamo, D; Castro, N.; Santana, M; González, F.; Cabrera, Fernando; Gracia, A.

doi:10.1016/j.theriogenology.2008.12.024

Cited by 48 publications

(38 citation statements)

References 29 publications

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“…Different studies have detected differences between bucks in the ability of the semen to remain viable after freezing (Leboeuf et al. 2000; Batista et al. 2009).…”

Section: Introductionmentioning

confidence: 99%

Influence of the Preservation Temperature (37, 20, 4, −196°C) and the Mixing of Semen over Sperm Quality of Majorera Bucks

Batista

Niño

Santana

et al. 2011

Reprod Domestic Animals

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This study assessed the effect of different semen storage temperatures and the influence of semen pooling in semen viability. In experiment 1, semen samples (n = 30) of five Majorera bucks were individually processed [Individual semen (IS)] and after the first dilution (Tris-yolk extender), semen-diluted aliquots from each male were pooled semen (PS). Thereafter, semen samples (IS and PS) were preserved as fresh semen (37 and 20°C), chilled semen (4°C) and frozen semen. Sperm motility and the percentage of abnormal sperm cells and intact membrane acrosomes were defined. Semen preservation at 20 and 4°C did not modify the quality of spermatozoa for the first 24 h, but the conservation at 37°C caused a dramatic fall in the semen motility from 12 h onwards. Furthermore, the longevity of frozen-thawed semen was limited to 4-6 h. No differences were observed in semen parameters when PS was compared with semen from individual males in any of the preservation protocols assessed. In experiment 2, 120 goats were distributed in four experimental groups: in group fresh individual semen (FIS, n = 30) and group frozen-thawed individual semen (FTIS, n = 30), does were transcervically inseminated with fresh semen and frozen-thawed semen from each individual male, respectively, and in group fresh pooled semen (FPS, n = 30) and group frozen-thawed pooled semen (FTPS, n = 30), goats were transcervically inseminated with FPS and FTPS, respectively. The kidding rate was very close in the FIS and FPS groups (70.0% and 73.7%, respectively), and no significant differences were observed in the fertility rate between FTIS and FTPS. The results of this study confirmed that semen samples may be preserved satisfactorily for 24 h both at 20 and 4°C. In addition, the mixture of semen of different bucks did not significantly modify the semen parameters when compared with semen from individual males.

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“…Different studies have detected differences between bucks in the ability of the semen to remain viable after freezing (Leboeuf et al. 2000; Batista et al. 2009).…”

Section: Introductionmentioning

confidence: 99%

Influence of the Preservation Temperature (37, 20, 4, −196°C) and the Mixing of Semen over Sperm Quality of Majorera Bucks

Batista

Niño

Santana

et al. 2011

Reprod Domestic Animals

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“…The kidding rate after artificial insemination with frozen-thawed goat semen ranges between 30% and 70%, while using fresh or chilled semen results in rates between 60% and 80%. 6 Thus, the commercial use of frozen-thawed semen in goat production is limited.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Effect of Various Disaccharides on Frozen Goat Spermatozoa

Quan

Hong

Lan

et al. 2012

Biopreservation and Biobanking

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In order to create a bank of goat spermatozoa, it was important to evaluate the protective effect of five different cryoprotectant extenders. Additionally, this study determined whether the cryoprotective effect of trehalose is superior to that of other disaccharides. The data indicate that the motility (~20%) and path velocity (~15 μm/s) of spermatozoa frozen with extenders containing disaccharide were significantly less than that of spermatozoa frozen with extender containing no disaccharide (45.21 ± 3.51% and 28.66 ± 13.57 μm/s, respectively; p<0.01). Moreover, in the presence of disaccharide, the percentage of goat spermatozoa with exposed phosphatidylserine following cryopreservation was over 35% and significantly higher than spermatozoa frozen in the absence of disaccharide (4.88 ± 3.41%, p<0.01). In addition, the presence of disaccharide did not increase the thermal resistance or protect membrane integrity of frozen spermatozoa. However, the percentage of cells with an intact acrosome following cryopreservation in the presence of disaccharide was significantly greater than that of cells frozen with no disaccharide (p<0.05). Finally, the protective effect of trehalose on frozen goat spermatozoa was similar to that of sucrose, maltose, or lactose (p>0.05). In conclusion, the effect of trehalose on frozen goat spermatozoa is not superior to that of other disaccharides including sucrose, maltose, or lactose. Moreover, disaccharide used as the main solute does not improve the quality of frozen goat spermatozoa except for protection of acrosome integrity. The role of disaccharide on frozen goat spermatozoa still needs further elucidation.

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“…In addition to the traditional method for freezing semen in straws, freezers operating with liquid nitrogen are also used (2,3,4,11). However, with the changes in life sciences, liquid nitrogen-based systems have faced with some problems, the most important two being the contamination risk and temperature change up to 90 °C (4,6,7,8,10). Therefore, there is a need to investigate feasible alternatives for semen cryopreservation which can prevent the complications of traditional methods (2).…”

mentioning

confidence: 99%

“…Therefore, there is a need to investigate feasible alternatives for semen cryopreservation which can prevent the complications of traditional methods (2). However, there are also other studies confirming that another viable alternative may be the use of ultra-low freezers at -152 °C, not too far from liquid nitrogen temperature at -196 °C (2,3,4,11). It remains to be established mechanical freezers which can provide air phase at -150°C without the need for liquid nitrogen can prevent cross-contamination of germ-plasm over a long period of storage (6,7).…”

mentioning

confidence: 99%

“…However, there are a few studies investigating the ultra-low freezer at -152 °C for freezing and storing canine and goat semen (2,3,4,11).One of the studies on the cryopreservation of canine semen assessed the post-thawing seminal parameters obtained by similar protocols including the use of ultra-low freezer at -152 °C and liquid nitrogen, finding no significant differences between both protocols in terms of sperm motility and percentages of dead and abnormal spermatozoa (2). When compared, the results of our study also detected no statistically significant differences between the similarly applied protocols (freezing over liquid nitrogen vapor and storing in liquid nitrogen, freezing over liquid nitrogen vapor and storing in ultra-low freezer, and freezing and storing in ultra-low freezer) in terms of sperm motility and percentages of dead and abnormal spermatozoa.…”

mentioning

confidence: 99%

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Effect of alternative cryopreservation procedures on bull semen

Korkmaz;DAŞKIN¹

2012

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The aim of this study was to assess the use of -152°C ultra freezer for freezing and storing bull semen as an alternative to freezing over the liquid nitrogen vapor and storing in liquid nitrogen in terms of certain post-thaw spermatological parameters. Two ejaculates, one per each bull, were used without pooling. Four different freezing and storing protocols were tested. At 1 week, 2 months and 6 months after cryopreservation, the frozen semen was thawed and the seminal parameters were determined. As a conclusion, this study has confirmed that the use of -152 °C ultra-low freezer for freezing and storing bull semen for 6 months is a viable alternative to liquid nitrogen.Key words: Bull, cryopreservation, semen, storage, ultra-low freezer. Alternatif Dondurma ve Saklama Yöntemlerinin Boğa Sperması Üzerine EtkisiÖzet: Bu çalışmanın amacı, boğa spermasının -152 °C derin dondurucuda dondurulup saklanmasının sıvı azot buharında dondurma ve sıvı azotta saklamaya çözüm sonu kimi spermatolojik parametreler bakımından bir alternatif olup olmayacağının araştırılmasıdır. Bu çalışmada iki adet boğanın birer ejakulatı miks yapılmadan kullanıldı. Araştırmada dört farklı dondurma ve saklama protokolü denendi. Dondurma işleminden bir hafta, iki ve altı aylık periyotlar sonrasında payetler çözdürüldü ve çözüm sonu bazı spermatolojik özellikler değerlendirildi. Sonuç olarak bu çalışma -152°C derin dondurucunun boğa spermasının dondurulması ve altı ay süreyle saklanmasında sıvı azota bir alternatif olarak kullanılabilirliğini ve boğa spermasının dondurulması ve saklanmasında iyi bir ortam sağladığını doğrulamaktadır. Anahtar sözcükler: Boğa, derin dondurucu, dondurma, saklama, sperma.* Aynı isimli doktora tezinden özetlenmiştir.Bull semen can be frozen in pellets, ampoules or straws and then stored in liquid nitrogen (13). In addition to the traditional method for freezing semen in straws, freezers operating with liquid nitrogen are also used (2,3,4,11). However, with the changes in life sciences, liquid nitrogen-based systems have faced with some problems, the most important two being the contamination risk and temperature change up to 90 °C (4,6,7,8,10). Therefore, there is a need to investigate feasible alternatives for semen cryopreservation which can prevent the complications of traditional methods (2). However, there are also other studies confirming that another viable alternative may be the use of ultra-low freezers at -152 °C, not too far from liquid nitrogen temperature at -196 °C (2,3,4,11). It remains to be established mechanical freezers which can provide air phase at -150°C without the need for liquid nitrogen can prevent cross-contamination of germ-plasm over a long period of storage (6,7). The aim of this experiment was to assess the use of an ultra-low freezer at -152 °C for freezing and storage of bull semen for 6 months. Postthawing seminal parameters were evaluated to see whether an ultra-low freezer at -152 °C could be a feasible alternative to cryopreservation in liquid nitrogen.Two Holstein bulls we...

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Successful artificial insemination using semen frozen and stored by an ultrafreezer in the Majorera goat breed

Cited by 48 publications

References 29 publications

Influence of the Preservation Temperature (37, 20, 4, −196°C) and the Mixing of Semen over Sperm Quality of Majorera Bucks

Influence of the Preservation Temperature (37, 20, 4, −196°C) and the Mixing of Semen over Sperm Quality of Majorera Bucks

Comparison of the Effect of Various Disaccharides on Frozen Goat Spermatozoa

Effect of alternative cryopreservation procedures on bull semen

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