Successful FCS Experiment in Nonstandard Conditions

Banachowicz, Ewa; Patkowski, A.; Meier, G. E. A.; Klamecka, Kamila; Gapiński, Jacek

doi:10.1021/la5015708

Cited by 29 publications

(30 citation statements)

References 10 publications

(28 reference statements)

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“…To improve the signal/noise ratio of the autocorrelation function, each measurement series was based on 40 scans of the diffusion time, with a duration of 15 s each. More details about the used setup can be found in Banachowicz et al (2014). The results obtained from the experiment were verified by simulation based on a simple bead model, performed in the Hydro++ program (Ortega et al 2007).…”

Section: Methodsmentioning

confidence: 99%

Comparison of excitation energy transfer in cyanobacterial photosystem I in solution and immobilized on conducting glass

et al. 2016

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Excitation energy transfer in monomeric and trimeric forms of photosystem I (PSI) from the cyanobacterium Synechocystis sp. PCC 6803 in solution or immobilized on FTO conducting glass was compared using time-resolved fluorescence. Deposition of PSI on glass preserves bi-exponential excitation decay of ~4–7 and ~21–25 ps lifetimes characteristic of PSI in solution. The faster phase was assigned in part to photochemical quenching (charge separation) of excited bulk chlorophylls and in part to energy transfer from bulk to low-energy (red) chlorophylls. The slower phase was assigned to photochemical quenching of the excitation equilibrated over bulk and red chlorophylls. The main differences between dissolved and immobilized PSI (iPSI) are: (1) the average excitation decay in iPSI is about 11 ps, which is faster by a few ps than for PSI in solution due to significantly faster excitation quenching of bulk chlorophylls by charge separation (~10 ps instead of ~15 ps) accompanied by slightly weaker coupling of bulk and red chlorophylls; (2) the number of red chlorophylls in monomeric PSI increases twice—from 3 in solution to 6 after immobilization—as a result of interaction with neighboring monomers and conducting glass; despite the increased number of red chlorophylls, the excitation decay accelerates in iPSI; (3) the number of red chlorophylls in trimeric PSI is 4 (per monomer) and remains unchanged after immobilization; (4) in all the samples under study, the free energy gap between mean red (emission at ~710 nm) and mean bulk (emission at ~686 nm) emitting states of chlorophylls was estimated at a similar level of 17–27 meV. All these observations indicate that despite slight modifications, dried PSI complexes adsorbed on the FTO surface remain fully functional in terms of excitation energy transfer and primary charge separation that is particularly important in the view of photovoltaic applications of this photosystem.

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Section: Methodsmentioning

confidence: 99%

Comparison of excitation energy transfer in cyanobacterial photosystem I in solution and immobilized on conducting glass

et al. 2016

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“…When the refractive indexes are different, the correction collar must be adjusted for each measurement separately. 12 Although formally eq 11 used to generate the τ c = f(a c ) curve is a cubic function and the value of a c corresponding to the measured τ c could be calculated analytically using Cardano's formulas, 16 it seems much more rational to solve it graphically by generating the data required to make the plot of τ c = f(a c ) with required accuracy. The same graphical method can be used to obtain the a c value from the c = f(a c ) dependence.…”

Section: Methodsmentioning

confidence: 99%

Size of Submicrometer Particles Measured by FCS: Correction of the Confocal Volume

Deptuła¹,

Buitenhuis

Jarzębski³

et al. 2015

Langmuir

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When fluorescence correlation spectroscopy (FCS) in combination with a confocal microscope is used to determine the hydrodynamic radius a of particles comparable to or larger than the linear size σ of the confocal volume of the microscope, a correction must be used that depends on the a(2)/σ(2) ratio and the distribution of the dye within the particle. Here we present the experimental validation of the theoretically predicted approximate correction necessary for appropriate measurements of the size of uniformly fluorescently labeled spheres of radius comparable to the size of the confocal volume. We also test the approximate correction formula for different ranges of the a/σ ratio and propose a simple procedure to obtain the correct nanoparticle size from such a measurement.

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“…In our previous publications [5,26] we have shown that the spherical aberration due to the diamond window thickness of 1 mm (refractive index n = 2.415) can be compensated by the correction collar of the LWD objective #1. We also demonstrated that this combination of objective #1 and a diamond window can be used for a successful FCS measurement.…”

Section: Fluorescence Correlation Spectroscopy (Fcs)mentioning

confidence: 95%

Nano-viscosity of supercooled liquid measured by fluorescence correlation spectroscopy: Pressure and temperature dependence and the density scaling

Meier

Gapiński

Ratajczyk

et al. 2018

The Journal of Chemical Physics

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Single particle experiments: fluorescence correlation spectroscopy (FCS) and particle tracking (PT) allow to obtain the nano-viscosity of the system from the measured self/tracer translational diffusion coefficients, using the Stokes-Einstein (SE) relation. In order to perform such measurements as function of pressure and temperature a new sample cell was designed and is described in this work. We show that this cell in combination with a long working distance objective of the confocal microscope can be used for successful FCS, PT and confocal imaging experiments in broad pressure (0.1-100 MPa) and temperature ranges. The temperature and pressure dependent nanoviscosity of a van der Waals liquid obtained from the translational diffusion coefficient measured in this cell by means of FCS obeys the same scaling as the rotational relaxation and macro-viscosity of the system.

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Successful FCS Experiment in Nonstandard Conditions

Cited by 29 publications

References 10 publications

Comparison of excitation energy transfer in cyanobacterial photosystem I in solution and immobilized on conducting glass

Comparison of excitation energy transfer in cyanobacterial photosystem I in solution and immobilized on conducting glass

Size of Submicrometer Particles Measured by FCS: Correction of the Confocal Volume

Nano-viscosity of supercooled liquid measured by fluorescence correlation spectroscopy: Pressure and temperature dependence and the density scaling

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