To introduce a new technique for creation of neo vagina in cases of MRKH Syndrome that offers good anatomic and functional results. Materials and Methods: 3 patients with complaints of primary amenorrhea and with MRKH syndrome were treated by using a novel technique of vaginoplasty i.e. peritoneal tube pull through technique. Results: In our study, all patients were followed for preferably 6 months (1, 4, 8, 12 weeks and if possible 6 months) after surgery for following observations-vaginal calibre, presence of any stricture, vaginal infection, failure of graft uptake or any urogenital complaints. All had excellent results. Conclusion: Peritoneal tube pull through technique for creation of neovagina is a safe and effective method for patients of MRKH Syndrome.