Successful plant regeneration system via de novo organogenesis in Syzygium cumini (L.) Skeels: an important medicinal tree

Naaz, Afshan; Hussain, Sheikh Altaf; Naz, Ruphi; Anis, Mohammad; Alatar, Abdulrahman A.

doi:10.1007/s10457-018-0236-4

Cited by 14 publications

(8 citation statements)

References 24 publications

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“…The frequency of shoot regeneration from node explants was the highest in MS medium containing (5.0 µ M) BA. The type of exogenously administered cytokinins, as well as their concentration, absorption, and transport, have an effect on the success of the in vitro propagation method [78,79]. The effect of BA on shoot induction and multiplication from node explants has been reported by many authors [67,74,[80][81][82][83][84].…”

Section: Discussionmentioning

confidence: 99%

High-Frequency Plant Regeneration, Genetic Uniformity, and Flow Cytometric Analysis of Regenerants in Rutachalepensis L.

Qahtan

Alatar

Abdel-Salam

2021

Plants

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Ruta chalepensis L., an evergreen shrub in the citrus family, is well-known around the world for its essential oils and variety of bioactivities, indicating its potential medicinal applications. In this study, we investigated the effect of different culture conditions, including plant growth regulators, media types, pH of the medium, and carbon sources, on in vitro regeneration from nodal explants of R. chalepensis. Following 8 weeks of culture, the highest percentage of regeneration (96.3%) and maximum number of shoots (40.3 shoot/explant) with a length of 4.8 cm were obtained with Murashige and Skoog (MS) medium at pH 5.8, supplemented with 3.0% sucrose and 5.0 µM 6-Benzyladenine (BA) in combination with 1.0 µM 1-naphthaleneacetic acid (NAA). For rooting, individually harvested shootlets were transferred on ½ MS (half-strength) supplemented with IAA (indole-3-acetic acid), IBA (indole 3-butyric acid), or NAA, and the best response in terms of root induction (91.6%), number of roots (5.3), and root mean length (4.9 cm) was achieved with 0.5 µM IBA after 6 weeks. An average of 95.2 percent of healthy, in vitro regenerated plantlets survived after being transplanted into potting soil, indicating that they were effectively hardened. DNA assays (PCR-based markers) such as random amplification of polymorphic DNA (RAPD) and directed amplification of minisatellite-region (DAMD) were employed to assess in vitro cultivated R. chalepensis plantlets that produced a monomorphic banding pattern confirming the genetic stability. Additionally, no changes in the flow cytometric profile of ploidy between regenerated plantlets and donor plants were detected. Regeneration of this valuable medicinal plant in vitro will open up new avenues in pharmaceutical biotechnology by providing an unconventional steadfast system for mass multiplication and might be effectively used in genetic manipulation for enhanced bioactive constituents.

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Section: Discussionmentioning

confidence: 99%

High-Frequency Plant Regeneration, Genetic Uniformity, and Flow Cytometric Analysis of Regenerants in Rutachalepensis L.

Qahtan

Alatar

Abdel-Salam

2021

Plants

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“…It has been found that auxins could not induce shoot multiplication when used alone and that the cytokinin unaided produced very few shoots, whereas the combination of cytokinin and auxin underwent profusion of regeneration. Many studies have shown that a lower dose of auxins is needed in combination with cytokinin to boost the multiplication rate in many plant species [ 83 , 84 , 212 , 213 , 214 ]. α-naphthalene acetic acid (NAA), indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA) are widely used in shoot multiplication, proliferation, and rooting cultures.…”

Section: Propagation Of Pterocarpus Marsupiummentioning

confidence: 99%

“…According to De Klerk et al [ 285 ], high doses of auxin pretreatment are needed during the initial root induction process, but in many cases during root elongation, a high dose of any growth regulator becomes inhibitory. A large number of researchers have proposed IBA as the best rooting hormone in many plant species like Kigelia pinnata [ 286 ], Terminalia arjuna [ 287 ], Albizia lebbeck [ 85 ], and Syzygium cumini [ 214 ]. On the other hand, in P. marsupium microshoots, Husain, Anis, and Shahzad [ 137 ] applied a two-step rooting protocol for in vitro root induction.…”

Section: Propagation Of Pterocarpus Marsupiummentioning

confidence: 99%

Biotechnological Advances in Pharmacognosy and In Vitro Manipulation of Pterocarpus marsupium Roxb.

Ahmad

Anis

et al. 2022

Plants

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Trees are vital resources for economic, environmental, and industrial growth, supporting human life directly or indirectly through a wide variety of therapeutic compounds, commodities, and ecological services. Pterocarpus marsupium Roxb. (Fabaceae) is one of the most valuable multipurpose forest trees in India and Sri Lanka, as it is cultivated for quality wood as well as pharmaceutically bioactive compounds, especially from the stem bark and heartwood. However, propagation of the tree in natural conditions is difficult due to the low percentage of seed germination coupled with overexploitation of this species for its excellent multipurpose properties. This overexploitation has ultimately led to the inclusion of P. marsupium on the list of endangered plant species. However, recent developments in plant biotechnology may offer a solution to the overuse of such valuable species if such advances are accompanied by technology transfer in the developing world. Specifically, techniques in micropropagation, genetic manipulation, DNA barcoding, drug extraction, delivery, and targeting as well as standardization, are of substantial concern. To date, there are no comprehensive and detailed reviews of P. marsupium in terms of biotechnological research developments, specifically pharmacognosy, pharmacology, tissue culture, authentication of genuine species, and basic gene transfer studies. Thus, the present review attempts to present a comprehensive overview of the biotechnological studies centered on this species and some of the recent novel approaches for its genetic improvement.

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“…The maximum technical efficiency (MTE) was obtained at concentrations of 2,544.81; 2,607.52; 2,283.33 and 2,300.47 mg L -1 of IBA, which provided respectively the highest rooting (92.42%); higher number of roots (1.52); longer root length (17.29 mm) and higher root dry mass (9.162 mg) (Figure 6 a-d), after 10 seconds of immersion of the base of the explant. The highest ex vitro rhizogenesis (80%) was achieved in shoots of Syzygium cumini (L.) Skeels treated with IBA (40.64 mg L -1 ) for 30 minutes (Naaz et al, 2019). Nodal shoots of shoots of Nyctanthes arbortristis L. pre-incubated in solid medium MS½ with IBA (1.21 mg L -1 ) for 1 week, followed by planting in solid medium MS½ free of growth regulator, produced 6.20 roots per explant (Ratha et al, 2020).…”

Section: Cernementioning

confidence: 99%

VITRO OF Melanoxylon brauna SCHOTT. MORPHOGENESIS: RESPONSIVENESS OF EXPLANTS TO PERMANENT AND TEMPORARY IMMERSION GROWTH REGULATORS

Silva

Simões

Baptista

et al. 2020

CERNE

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Successful plant regeneration system via de novo organogenesis in Syzygium cumini (L.) Skeels: an important medicinal tree

Cited by 14 publications

References 24 publications

High-Frequency Plant Regeneration, Genetic Uniformity, and Flow Cytometric Analysis of Regenerants in Rutachalepensis L.

High-Frequency Plant Regeneration, Genetic Uniformity, and Flow Cytometric Analysis of Regenerants in Rutachalepensis L.

Biotechnological Advances in Pharmacognosy and In Vitro Manipulation of Pterocarpus marsupium Roxb.

VITRO OF Melanoxylon brauna SCHOTT. MORPHOGENESIS: RESPONSIVENESS OF EXPLANTS TO PERMANENT AND TEMPORARY IMMERSION GROWTH REGULATORS

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