Successful Prenatal Diagnosis from Maternal Blood with Magnetic‐Activated Cell Sorting

Gänshirt, Dorothee; Börjesson‐Stoll, Regina; Burschyk, Monika; Garritsen, Henk; Neusser, Marcus; Smeets, F. W. M.; Velasco, Marta; Walde, Corinna; Holzgreve, Wolfgang

doi:10.1111/j.1749-6632.1994.tb55753.x

Cited by 34 publications

(20 citation statements)

References 5 publications

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“…In order to solve the first problem, many groups are currently working on techniques to enrich for NRBCs through flow-sorting and/or magnetic sorting and subsequent PCR-or FISH-analysis (1,7,14,19). However, enrichment procedures inevitably lead to cell loss (1,6,18).…”

mentioning

confidence: 99%

Development of a preparation and staining method for fetal erythroblasts in maternal blood: Simultaneous immunocytochemical staining and FISH analysis

Oosterwijk¹,

Mesker²,

Velzen³

et al. 1998

Cytometry

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mentioning

confidence: 99%

Development of a preparation and staining method for fetal erythroblasts in maternal blood: Simultaneous immunocytochemical staining and FISH analysis

Oosterwijk¹,

Mesker²,

Velzen³

et al. 1998

Cytometry

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“…The stability of the NRBCs up to 180 hours in stored samples highlighted the effectiveness of choosing fetal erythroblasts as the ideal candidates for noninvasive diagnosis. 13 In 5 cases, we were able to isolate Most of the PCR defined methods reported earlier were restricted in their application only to sex-linked disorders and to pregnancies with male fetuses. Noninvasive prenatal diagnosis could be used to its maximum if fetal cells from the maternal circulation are used for genetic analysis of autosomal disorders.…”

Section: Discussionmentioning

confidence: 97%

Evaluation of the Use of Monoclonal Antibodies and Nested PCR for Noninvasive Prenatal Diagnosis of Hemoglobinopathies in India

et al. 2008

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Our purpose was to develop and evaluate isolation and enrichment of fetal erythroblasts and a nested polymerase chain reaction (PCR) approach using fetal erythroblasts for detecting the beta-globin gene mutations for a noninvasive prenatal diagnosis of hemoglobinopathies. Maternal blood at different periods of gestation was layered on a Percoll density gradient for enrichment of fetal nucleated RBCs (NRBCs). A combination of 3 monoclonal antibodies (CD45-peridinin chlorophyll protein, glycophorin A-phycoerythrin, and anti-hemoglobin F-fluorescein isothiocyanate) was used for flow cytometric sorting of fetal NRBCs from enriched cells. Different nested PCR-based approaches were used for identification of fetal mutations. Owing to heterogeneity of beta-thalassemia mutations in the population of India, we had to screen for 12 mutations and were able to give an accurate diagnosis in 84 (84.0%) of 100 cases when compared with chorionic villus sampling or cordocentesis and DNA analysis.This nested PCR approach enabled amplification of small quantities of DNA from fetal erythroblasts, providing a cost-effective method for noninvasive diagnosis.

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“…This difference may well be due to differences in target cells, in type and technique of invasive procedure (amniocentesis, transabdominal CVS, or transcervical CVS), and in methodology. Ganshirt et al (19), for instance, did not sample preand postinvasively in the same pregnancy, but only compared different groups. FIG.…”

Section: Discussionmentioning

confidence: 98%

Fetal cell detection in maternal blood: A study in 236 samples using erythroblast morphology, DAB and Hbf staining, and FISH analysis

et al. 1998

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Successful Prenatal Diagnosis from Maternal Blood with Magnetic‐Activated Cell Sorting

Cited by 34 publications

References 5 publications

Development of a preparation and staining method for fetal erythroblasts in maternal blood: Simultaneous immunocytochemical staining and FISH analysis

Development of a preparation and staining method for fetal erythroblasts in maternal blood: Simultaneous immunocytochemical staining and FISH analysis

Evaluation of the Use of Monoclonal Antibodies and Nested PCR for Noninvasive Prenatal Diagnosis of Hemoglobinopathies in India

Fetal cell detection in maternal blood: A study in 236 samples using erythroblast morphology, DAB and Hbf staining, and FISH analysis

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