Successful transplant of mesenchymal stem cells in induced osteonecrosis of the ovine femoral head: preliminary results

Abstract

PURPOSE: Evaluate the bone tissue recovery following transplantation of ovine mesenchymal stem cells (MSC) from bone marrow and human immature dental-pulp stem cells (hIDPSC) in ovine model of induced osteonecrosis of femoral head (ONFH). METHODS: Eight sheep were divided in three experimental groups. First group was composed by four animals with ONFH induced by ethanol through central decompression (CD), for control group without any treatment. The second and third group were compose by two animals, six weeks after ONFH induction received transplantation of heterologous ovine MSC (CD + oMSC), and hIDPSC (CD + hIDPSC), respectively. In both experiments the cells were transplanted without application of any type of immunosupression protocol. RESULTS: Our data indicate that both cell types used in experiments were able to proliferate within injured site providing bone tissue recovery. The histological results obtained from CD+hIDPSC suggested that the bone regeneration in such animals was better than that observed in CD animals. CONCLUSION: Mesenchymal stem cell transplant in induced ovine osteonecrosis of femoral head by central decompression technique is safe, and apparently favors bone regeneration of damaged tissues.

Protocols for obtainment and isolation of two mesenchymal stem cell sources in sheep

“…The first protocol, drawn up according to Feitosa 23 (bone marrow of sheep), was ineffective since it did not enable cell adherence and viability. The second protocol, based on methods used by Fuchs et al 14 showed formation of a halo of white and red blood cell supernatants that persisted even after washing the material.…”

Aspecto radiológico e macroscópico de matriz óssea mineralizada heteróloga fragmentada e polimetilmetacrilato autoclavados em falha óssea de tíbia de coelho

“…Uma nova opção para reparar grandes falhas ósseas seria o emprego de um compósito constituído pela associação de materiais biocompatíveis biológico (tecido ósseo heterólogo) e sintético (polimetilmetacrilato), com propriedades osteogênicas, osteoindutoras e osteocondutoras, que não favorecesse infecção nem a colonização bacteriana, que proporcionasse sustentação mecânica, que fosse biomodelado, de fácil aquisição e baixo custo, que não necessitasse de meio especializado para sua preservação, que preenchesse completamente a falha óssea, eliminando, com isso, problemas inerentes ao enxerto autógeno (Friedlaender 1982, Khan 2000, Alievi et al 2007, Feitosa et al 2010, Turrer & Ferreira, 2008, 2013a.…”

Platelet-rich plasma combined with core decompression and allogeneic fibula rod support for the treatment of osteonecrosis of the femoral head (Ficat stage II): a short-term follow-up

“…In the initial stage of ONFH, core decompression, together with allogeneic fibula rod support, is considered an effective surgical intervention. However, However, this treatment retains the femoral head in only 60-80% of cases in the short and medium term (12) . Femoral head core decompression involves drilling a tunnel to reduce the pressure on the femoral head, thereby alleviating pain.…”