Successful treatment of a 95 per cent body surface area burn

Robenpour, M.; Teman, J.; Tamir, Gabriel; Hauben, Daniel J.

doi:10.1016/0305-4179(90)90078-b

Cited by 11 publications

(4 citation statements)

References 10 publications

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“…Morbidity and mortality of patients with massive burns are closely related to the limited availability of donor sites [1]. Conventional treatments to promote recovery of these patients involve harvesting and grafting split-thickness skin grafts [2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

EDC cross-linking improves skin substitute strength and stability

Powell¹,

Boyce²

2006

Biomaterials

228

162

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Section: Introductionmentioning

confidence: 99%

EDC cross-linking improves skin substitute strength and stability

Powell¹,

Boyce²

2006

Biomaterials

228

162

View full text Add to dashboard Cite

“…Infection is the main cause of wound deepening (1). Morbidity and mortality of patients with massive burns are closely related to the limited availability of donor sites (2). Autografting has been considered as a better treatment for excised burn wounds, but donor sites for autograft are limited in patients with very large burns (3).…”

mentioning

confidence: 99%

Tissue‐Engineered Skin Containing Mesenchymal Stem Cells Improves Burn Wounds

et al. 2008

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There is increasing evidence showing that adult stem cells are useful for tissue regeneration. Bone marrow mesenchymal stem cells (MSCs) are self-renewing and are potent in differentiating into multiple cells and tissues. To investigate the practicability of repairing burn wounds with tissue-engineered (TE) skin combined with bone MSCs, we established a burn wound model in the porcine skin. With a controlling temperature and time of the burning device to obtain different degrees of burn wounds, a deep dermal partial thickness burn was introduced to the porcine skin using a heated-brass contact injury at 100 degrees C for 20 s. Collagen-GAG scaffolds were utilized as the matrix; MSCs separated from pigs were seeded on them to form the skin equivalent. When grafted to the burn wounds, the TE skin containing MSCs showed better healing and keratinization, less wound contraction, and more vascularization. Grafts proliferated well and contributed to the neo-tissues. These data suggest that TE skin containing MSCs in a burn defect can accelerate wound healing and receive satisfactory effects.

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“…While these autografts have inherent biocompatibility, harvesting them is associated with various comorbidities including prolonged immobility and donor site pain . Moreover, autograft availability is often not feasible with extensive burn wounds covering a large percentage of total body surface area .…”

Section: Introductionmentioning

confidence: 99%

Delivery of Allogeneic Adipose Stem Cells in Polyethylene Glycol-Fibrin Hydrogels as an Adjunct to Meshed Autografts After Sharp Debridement of Deep Partial Thickness Burns

Burmeister

Stone

Wrice

et al. 2018

Stem Cells Translational Medicine

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Harvesting of autografts results in donor site morbidities and is limited in scenarios such as large total body surface area burns. In these instances, coverage is increased by meshing grafts at the expense of delayed biologic closure. Moreover, graft meshing increases the likelihood of contraction and hypertrophic scarring, limits range of motion, and worsens cosmesis. Many tissue engineering technologies have touted the promise of adipose‐derived stem cells (ASCs) for burn wounds. The primary objective of the current study was to determine feasibility and efficacy of in situ ASC delivery via PEGylated fibrin (FPEG) hydrogels as adjuncts to meshed split thickness skin grafts in a porcine model. Deep partial thickness burns were created on the dorsum of anesthetized Yorkshire pigs, and subsequently debrided on post‐burn day 4. After debridement, wounds were treated with: split thickness skin grafts (STSG); meshed STSG (mSTSG); and mSTSG + FPEG with increasing doses of ASCs. We show that FPEG hydrogels can be delivered in situ to prevent the contraction seen after meshing of STSG. Moreover, ASCs delivered in FPEG dose‐dependently increase blood vessel size which significantly correlates with CD31 protein levels. The current study reports a dual‐action adjunct therapy to autografting administered in situ, wherein FPEG acts as both scaffolding to prevent contraction, and as a delivery vehicle for ASCs to accelerate angiogenesis. This strategy may be used to incorporate other biologics for generating tissue engineered products aimed at improving wound healing and minimizing donor sites or scarring. stem cells translational medicine 2018;7:360–372

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Successful treatment of a 95 per cent body surface area burn

Cited by 11 publications

References 10 publications

EDC cross-linking improves skin substitute strength and stability

EDC cross-linking improves skin substitute strength and stability

Tissue‐Engineered Skin Containing Mesenchymal Stem Cells Improves Burn Wounds

Delivery of Allogeneic Adipose Stem Cells in Polyethylene Glycol-Fibrin Hydrogels as an Adjunct to Meshed Autografts After Sharp Debridement of Deep Partial Thickness Burns

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