Successful vitrification of early-stage porcine cloned embryos

Transcriptome Analysis of mRNAs and Long Non-Coding RNAs During Subsequent Embryo Development of Porcine Cloned Zygotes After Vitrification

“…In our previous study, the porcine cloned zygotes after vitrification showed a reduced embryo development in vitro, and abnormal expression of mRNA levels was also found in the resultant blastocysts (Jia et al, 2020). Therefore, it is important to continue in-depth research, focusing on the dynamic transcriptome landscapes in different developmental stages of these vitrified zygotes.…”

“…According to our previous study (Jia et al, 2020), the presumptive cloned zygotes after chemical activation were submitted to vitrification and warming. Basic medium (BM) was composed of DPBS with 20% (v/v) knockout serum replacement.…”

“…Some studies have observed that vitrification of porcine early embryos including zygotes and 2-and 4-cells reduces their potential to develop to the blastocyst stage (Esaki et al, 2004;Somfai et al, 2009;Gomis et al, 2013). Although not reported frequently, there are some studies being conducted on the vitrification of porcine cloned embryos, resulting in an acceptable survival rate (Du et al, 2007;Kawakami et al, 2008;Nakano et al, 2011;Jia et al, 2020). Moreover, porcine cloned embryos may be appropriate for vitrifying at very early developmental stages such as the zygote stage, so they can be well applied to embryo transfer or mechanism research relate to embryonic development.…”

“…Moreover, porcine cloned embryos may be appropriate for vitrifying at very early developmental stages such as the zygote stage, so they can be well applied to embryo transfer or mechanism research relate to embryonic development. Although vitrification is proved to not significantly reduce survival rate, the porcine cloned zygotes still show lower blastocyst formation rate as compared with their fresh counterparts (27.1 vs. 36.7%) (Jia et al, 2020). Thus it is very necessary to explore the structural and functional changes that occur during embryonic development of vitrified porcine cloned zygotes.…”

“…For instance, vitrification of porcine blastocysts has been reported to result in altered expression of POU5F1 and HSPA1A (Castillo-Martín et al, 2015). In our previous study, the resultant blastocysts from vitrified early-stage porcine cloned embryos showed increased expression of PCNA, CPT1, POU5F1 and DNMT3B (Jia et al, 2020). Moreover, a microarray approach has also been applied to analyze the impact of vitrification on transcriptome profile of in vivo-derived porcine blastocysts (Cuello et al, 2021).…”