Salidroside improves porcine oocyte maturation and subsequent embryonic development by promoting lipid metabolism

Li, Jiannan; Wang, Ruibin; Chen, Qiang; Tian, Yi-Qi; Gao, Leilei; Lei, Anmin

doi:10.1016/j.theriogenology.2022.08.028

Cited by 9 publications

(6 citation statements)

References 43 publications

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“…Numerous studies have been conducted over the past decade to investigate the medicinal properties of Sal, and anticancer, antioxidative, antiaging, antidiabetic, antidepressant, antihyperlipidemic, anti-inflammatory, and immunomodulatory effects were reported [ 19 ]. Although there have been studies indicating that Sal improves the lipid content in oocytes [ 20 ], we found that Sal promotes oocyte maturation while increasing mitochondrial activity, while reducing ROS and GSH levels in oocytes. This is reflected in aspects such as maturation, metabolism, cell communication, apoptosis, and oxidative stress.…”

Section: Discussioncontrasting

confidence: 65%

Salidroside Supplementation Affects In Vitro Maturation and Preimplantation Embryonic Development by Promoting Meiotic Resumption

Shi,

Geng,

et al. 2023

Genes

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Salidroside (Sal) possesses several pharmacological activities, such as antiaging, and anti-inflammatory, antioxidant, anticancer activities, and proliferation-promoting activities, but the effects of Sal on oocytes have rarely been reported. In the present study, we evaluated the beneficial effects of Sal, which is mainly found in the roots of Rhodiola. Porcine cumulus oocyte complexes were cultured in IVM medium supplemented (with 250 μmol/L) with Sal or not supplemented with Sal. The maturation rate in the Sal group increased from 88.34 ± 4.32% to 94.12 ± 2.29%, and the blastocyst rate in the Sal group increased from 30.35 ± 3.20% to 52.14 ± 7.32% compared with that in the control group. The experimental groups showed significant improvements in the cumulus expansion area. Sal reduced oocyte levels of reactive oxygen species (ROS) and enhanced intracellular GSH levels. Sal supplementation enhanced the mitochondrial membrane potential (MMP), ATP level, and mtDNA copy number, which shows that Sal enhances the cytoplasmic maturation of oocytes. Oocytes in the Sal group exhibited slowed apoptosis and reduced DNA breakage. Cell cycle signals and oocyte meiosis play important roles in oocyte maturation. The mRNA expressions of the MAPK pathway and MAPK phosphorylation increased significantly in the Sal group. The mRNA expression of the oocyte meiosis gene also increased significantly. These results show that Sal enhances the nuclear maturation of oocytes. Moreover, Sal increased the number of blastocyst cells, the proliferation of blastocysts, and the expressions of pluripotency genes. Sal down-regulated apoptosis-related genes and the apoptotic cell rate of blastocysts. In summary, our results demonstrate that Sal is helpful to improving the quality of porcine oocytes in vitro, and their subsequent embryonic development.

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Section: Discussioncontrasting

confidence: 65%

Salidroside Supplementation Affects In Vitro Maturation and Preimplantation Embryonic Development by Promoting Meiotic Resumption

Shi,

Geng,

et al. 2023

Genes

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“…Oxidative stress also disrupts the mitochondrial membrane potential, which reduces the oxidative phosphorylation reaction and ATP synthesis ( Aiken, Cindrova-Davies & Johnson, 2008 ). Numerous studies have reported that antioxidant supplementation during IVM is commonly able to overcome mitochondria injury by reducing ROS levels ( Chen et al, 2022 ; Li et al, 2022 ; Liu et al, 2023 ). A previous study reported that Lut increased PGC-1α and nitric oxide levels, which would correct cellular impairment and may reduce mitochondrial ROS production, suggesting that Lut may promote mitochondrial biogenesis ( Suh, Chon & Choi, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos

Jeong

Yang

Jeon

et al. 2023

PeerJ

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Luteolin (Lut), a polyphenolic compound that belongs to the flavone subclass of flavonoids, possesses anti-inflammatory, cytoprotective, and antioxidant activities. However, little is known regarding its role in mammalian oocyte maturation. This study examined the effect of Lut supplementation during in vitro maturation (IVM) on oocyte maturation and subsequent developmental competence after somatic cell nuclear transfer (SCNT) in pigs. Lut supplementation significantly increased the proportions of complete cumulus cell expansion and metaphase II (MII) oocytes, compared with control oocytes. After parthenogenetic activation or SCNT, the developmental competence of Lut-supplemented MII oocytes was significantly enhanced, as indicated by higher rates of cleavage, blastocyst formation, expanded or hatching blastocysts, and cell survival, as well as increased cell numbers. Lut-supplemented MII oocytes exhibited significantly lower levels of reactive oxygen species and higher levels of glutathione than control MII oocytes. Lut supplementation also activated lipid metabolism, assessed according to the levels of lipid droplets, fatty acids, and ATP. The active mitochondria content and mitochondrial membrane potential were significantly increased, whereas cytochrome c and cleaved caspase-3 levels were significantly decreased, by Lut supplementation. These results suggest that Lut supplementation during IVM improves porcine oocyte maturation through the reduction of oxidative stress and mitochondria-mediated apoptosis.

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“…The simplified experimental flow of our study design ( Figure 1 a) supports the field of mammalian reproduction by providing a framework for controlled experiments, such as testing the efficacy of unknown chemicals on mammalian oocyte maturation without FF or serum. However, numerous studies have been published regarding the key factors of pFF [ 16 , 19 , 20 , 21 ] and tests for unknown chemicals during mammalian oocyte maturation or embryo development with or without defined media [ 22 , 23 , 24 , 25 ]. Although studies using defined media may be suitable for testing unknown chemicals, many have shown poor oocyte development.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Porcine Follicular Fluid-Derived Exosome: The Pivotal Material for Porcine Oocyte Maturation in Lipid Antioxidant Activity

Kim

Lee

2023

IJMS

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Several studies have examined exosomes derived from porcine follicular fluid (FF), but few have reported their application in controlled experiments. The main concern in the field of embryology may be that controlled conditions, such as using a defined medium intermittently, cause poor results in mammalian oocyte maturation and embryo development. The first reason is the absence of the FF, which copes with the majority of the processes emerging in oocytes and embryos. Therefore, we added exosomes derived from porcine FF to the maturation medium of porcine oocytes. For morphological assessment, cumulus cell expansion and subsequent embryonic development were evaluated. Moreover, several stainings, such as glutathione (GSH) and reactive oxygen species (ROS), fatty acid, ATP, and mitochondrial activity, as well as evaluations of gene expression and protein analysis, were used for the functional verification of exosomes. When the oocytes were treated with exosomes, the lipid metabolism and cell survival of the oocytes were fully recovered, as well as morphological evaluations compared to the porcine FF-excluded defined medium. Therefore, controlled experiments may produce reliable data if the exosomes are treated with the desired amounts, and we suggest applying FF-derived exosomes to promote experimental data when performing controlled experiments in embryology.

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Salidroside improves porcine oocyte maturation and subsequent embryonic development by promoting lipid metabolism

Cited by 9 publications

References 43 publications

Salidroside Supplementation Affects In Vitro Maturation and Preimplantation Embryonic Development by Promoting Meiotic Resumption

Salidroside Supplementation Affects In Vitro Maturation and Preimplantation Embryonic Development by Promoting Meiotic Resumption

Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos

Porcine Follicular Fluid-Derived Exosome: The Pivotal Material for Porcine Oocyte Maturation in Lipid Antioxidant Activity

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