Successful vitrification of pronuclear-stage rabbit zygotes by minimum volume cooling procedure

Hochi, Shinichi; Terao, T.; Kamei, M; Kato, Megumi; Hirabayashi, Masumi; Hirao, Masao

doi:10.1016/s0093-691x(03)00232-2

Cited by 68 publications

(63 citation statements)

References 19 publications

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“…Three major methods have been used to preserve embryos: slow freezing, conventional vitrification or an adaptation of the last one, vitrification in open pulled straws (OPS) [149] or in other specialized embryo vitrification devices (copper or gold electron microscope grids [150] and pipette tips and cryotop [151,152], among others) referred as an ultrarapid vitrification. The major difference among them is the acceleration of the speed of freezing.…”

Section: Cryopreservation Of Sheep Embryosmentioning

confidence: 99%

Cryopreservation of Sheep Produced Embryos – Current and Future Perspectives

Romão¹,

Marques²,

Bettencourt³

et al. 2016

Insights From Animal Reproduction

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Due to economical and scientific limitations, sheep embryo reproductive technologies are less commercially applied than in other animal species. However, it is very clear that, in the near future, those techniques are expected to have a central role in animal production as a consequence of genetic and reproductive demands. One drawback is that results obtained after sheep embryo cryopreservation are unattractive for commercial purposes. It is expected that a successful cryopreservation of sheep embryos can push forward all other reproductive biotechnologies in this species, such as multiple ovulation and embryo transfer (MOET), artificial insemination, or in vitro production of embryos. This paper tries to discuss the current and future perspectives of cryopreservation of in vivo-and in vitro-produced sheep embryos concerning advantages and limitations for its practical use and possible solutions for improving methods to allow a higher survival rate of cryopreserved embryos.

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Section: Cryopreservation Of Sheep Embryosmentioning

confidence: 99%

Cryopreservation of Sheep Produced Embryos – Current and Future Perspectives

Romão¹,

Marques²,

Bettencourt³

et al. 2016

Insights From Animal Reproduction

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show abstract

“…Embryos may pass rapidly through the dangerous zone (temperature range from +20 to -20 o C), which decreases the risk of chilling injury. This method has been applied to various species for embryo cryopreservation, including the cow (Chian et al, 2004), rabbit (Hochi et al, 2004), buffalo (Muenthaisong et al, 2007), pig (Du et al, 2007) and human (Antinori et al, 2007).…”

Section: Cryotop (Also Called Minimum-volume Cooling)mentioning

confidence: 99%

Oocyte and embryo cryopreservation – state of art and recent developments in domestic animals

Gajda¹,

Smorąg²

2009

J. Anim. Feed Sci.

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During the past few decades, significant progress in cryopreservation of mammalian oocytes and embryos has been observed. Transfer of cryopreserved embryos or oocytes resulted in live offspring in at least 25 species. So far, two major methods have been used for oocyte and embryo cryopreservation: conventional slow-rate freezing and vitrification. This review summarizes the progress in cryopreservation of mammalian oocytes and embryos that has been achieved by modifying oocyte/embryo susceptibility to cryopreservation or vitrification technology through such techniques as solid-surface vitrification, cryoloop, microdrop, cryotop, electron microscopy grids, nylon mesh, open pulled straw method or removal of lipids, addition of antifreeze protein or cytoskeletonstabilizing agents, cholesterol or liposomes, centrifugation prior to cryopreservation or application of high hydrostatic pressure. In conclusion, the vitrification method opened new perspectives in cryopreservation of embryos and oocytes, both for in vitro fertilized and somatic nuclear transfer. Authors believe that new cryopreservation procedures which modify the susceptibility of oocytes and embryos to cryopreservation will gain importance as a major tool in mammalian gamete and embryo cryopreservation.

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“…Pese a su efecto favorable, estas sustancias pueden tener efectos nocivos, ya que pueden causar un shock osmótico (especialmente en altas concentraciones) y suelen resultar tóxicos para las células. Esta toxicidad se presenta en mayor o menor medida en función del peso molecular y de la temperatura a la que son aplicados estos agentes (Massip, 2001), así como del tiempo de exposición de los embriones al medio ; de hecho, se ha observado un mayor efecto perjudicial en la exposición de embriones a una solución con un 30% de crioprotector durante 10 minutos, frente a una del 40% durante dos minutos (Hochi et al, 2004). Pero no sólo la adición correcta del crioprotector es importante, la eliminación del mismo es también imprescindible cuando se descongela o desvitrifica una célula, por tanto, la velocidad de descongelación o desvitrificación también debe ser la adecuada (Shaw and Jones, 2003).…”

Section: Crioprotectoresunclassified

“…Para ello, de entre las diferentes opciones que pueden llevarse a cabo en la valoración de los embriones de conejo Chrenek et al, 1998;Cheng et al, 1999;Silvestre et al, 2003;Vicente et al, 2003;Hochi et al, 2004;Makarevich et al, 2007) probablemente la más frecuente por su simplicidad y por la información que aporta es el cultivo in vitro de los mismos, considerando viables aquéllos que alcanzan el estadio de blastocisto expandido o escapado en el tiempo adecuado, dada la relación que existe entre el tiempo de la primera división post-inseminación y la competencia de desarrollo .…”

Section: Viabilidadunclassified

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Efecto De La Aplicación De Gonadotropinas Recombinantes Humanas Sobre La Producción Y Calidad De Ovocitos Y Embriones en Coneja

Nicolau¹

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Successful vitrification of pronuclear-stage rabbit zygotes by minimum volume cooling procedure

Cited by 68 publications

References 19 publications

Cryopreservation of Sheep Produced Embryos – Current and Future Perspectives

Cryopreservation of Sheep Produced Embryos – Current and Future Perspectives

Oocyte and embryo cryopreservation – state of art and recent developments in domestic animals

Efecto De La Aplicación De Gonadotropinas Recombinantes Humanas Sobre La Producción Y Calidad De Ovocitos Y Embriones en Coneja

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