Succession of bacterial microbiota in tilapia fillets at 4 °C and in situ investigation of spoilers

Duan, Shengwen; Xing-zhi, Zhou; Miao, Jianyin; Duan, Xingxing

doi:10.1007/s11274-018-2452-5

Cited by 14 publications

(13 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To some extent, the bacterial genera found at 4 °C in this research differ from the findings of the authors’ previous research (Duan et al., ), which found Pseudomonas to be the most dominant genus throughout the storage process, while Shewanella and Psychrobacter were only found to be dominant after 3 days of storage, and Acinetobacter , Brevibacterium , Flavobacterium , Dietzia , and Janthinobacterium were the minor genera throughout all periods of detection. Yet, it is important to note that different sources of the tilapia fillets were used in the present and previous researches.…”

Section: Resultscontrasting

confidence: 99%

“…Finally, the amplified 16S rDNA fragments were analyzed by DGGE according to the method outlined in Duan et al. ().…”

Section: Methodsmentioning

confidence: 99%

“…DNA extraction. Extraction of bacterial DNA was performed according to Duan, Zhou, Miao, and Duan (2018). Briefly, the bacterial cells were washed from the tilapia fillet samples with 0.9 % saline.…”

Section: Analysis Of Microbiota By Pcr-dggementioning

confidence: 99%

“…The amplified products were subjected to agarose gel electrophoresis, in which a DNA Marker (DL2000, TaKaRa Biotechnology (Dalian) Co., Ltd., Dalian, China) was used to check the yield and integrity. Finally, the amplified 16S rDNA fragments were analyzed by DGGE according to the method outlined in Duan et al (2018).…”

Section: Pcr Amplification Of 16s Rdna Fragments and Dgge Analysismentioning

confidence: 99%

See 3 more Smart Citations

Characterization of Bacterial Microbiota in Tilapia Fillets Under Different Storage Temperatures

Duan

Xing-zhi

Xiao

et al. 2019

Journal of Food Science

Self Cite

View full text Add to dashboard Cite

This paper investigates the bacterial microbiota in tilapia fillets under cold (4 °C), iced (0 °C), and superchilled (–3 °C) storage conditions. At 4 °C, at least seven species/strains of Pseudomonas were detected in the fillets, five of which were dominant either at a certain stage or throughout the entire storage period. Shewanella was less dominant than Pseudomonas at 4 °C, while Serratia became dominant after 6 days storage at 4 °C. The microbiota in fillets stored at 0 and –3 °C were very similar and rarely changed during storage, yet differed greatly from the microbiota at 4 °C. Only two Pseudomonas species/strains grew at 0 and –3 °C, one of which was the most dominant. A Vibrionimonas sp. not found at 4 °C was found to be the second most dominant species at 0 and –3 °C. Shewanella and Psychrobacter were also present at 0 and –3 °C but were the minor genera. The most dominant strains at –3, 0, and 4 °C were separately isolated and subjected to full length 16S rDNA sequencing, which demonstrated that they were identical and were Pseudomonas fluorescens. The changes of the total bacterial count and TVBN value of the fillets inoculated with the isolated P. fluorescens were very similar to those of fillets with natural microbiota. This implies that P. fluorescens is the most important spoiler of tilapia fillets at –3, 0, or 4 °C. Practical Application This research shows that fewer species of bacteria survive at 0 and –3 °C than those at 4 °C, while among these bacteria, the most important spoiler is P. fluorescens. This may provide some clues to extend the shelf life of tilapia fillets by taking some inhibitory measures targeted at P. fluorescens in the future.

show abstract

Section: Resultscontrasting

confidence: 99%

“…Finally, the amplified 16S rDNA fragments were analyzed by DGGE according to the method outlined in Duan et al. ().…”

Section: Methodsmentioning

confidence: 99%

Section: Analysis Of Microbiota By Pcr-dggementioning

confidence: 99%

Section: Pcr Amplification Of 16s Rdna Fragments and Dgge Analysismentioning

confidence: 99%

See 2 more Smart Citations

Characterization of Bacterial Microbiota in Tilapia Fillets Under Different Storage Temperatures

Duan

Xing-zhi

Xiao

et al. 2019

Journal of Food Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…It was concluded that the techniques used in the research were suitable for the purpose of the study due to the cost, time and prior knowledge required to perform the analysis. Duan et al (2018) investigated the succession of bacterial microbiota in tilapia fillets during cold storage at 4°C applying the PCR-DGGE method.…”

Section: Denaturation Gradient Gel Electrophoresis (Dgge)mentioning

confidence: 99%

Untitled

Mısır¹

2018

GenAqua

View full text Add to dashboard Cite

Global seafood consumption has significantly increased due to the health concerns of populations. One of the basic requirements to meet the increasing demand for seafood is security and safety. To meet these requirements, detection and identification of seafood pathogens are of importance. For this purpose, besides conventional methods a variety of rapid, sensitive and reliable techniques have been developed. In general, rapid detection methods are generally time-efficient, sensitive, specific and labor-saving. This review emphasizes on the principles and applications of recent rapid methods for the detection of seafood pathogens; including polymerase chain reaction (PCR), multiplex PCR, Random Amplified Polymorphic DNA (RAPD), Real Time Polymerase Chain Reaction or Quantitative Polymerase Chain Reaction (qPCR), Denaturation Gradient Gel Electrophoresis (DGGE), loop-mediated isothermal amplification (LAMP).

show abstract

Assessing the inhibitory activity of culture supernatants against foodborne pathogens of two psychrotrophic bacteria isolated from river trout

et al. 2022

View full text Add to dashboard Cite

There is a need for new natural products with antimicrobial activity to treat multidrug resistant bacteria that can cause human illness. Some of them are foodborne pathogens. Two different Gram-negative psychrotrophic strains were isolated from healthy trout river samples (Salmotrutta). Based on phenotypic characterization, proteomics, genotyping and phylogenetic analyses of 16 rRNA gene, strains TCPS12 and TCPS13 were identified as Shewanellabaltica and Pseudomonasfragi, respectively. Both of them produced an exopolysaccharide that showed antimicrobial activity against four foodborne pathogens. P. fragi supernatant (AS13) showed higher antimicrobial activity than S. baltica supernatant (AS12) against all tested pathogens. The stability of the antimicrobial activity of AS13 was assessed against Enterococcus faecalis ATCC 29212 under different conditions. This solution was stable when exposed for 30 min to temperatures ranging from 40 to 100 °C. In addition, it retained its activity within a pH range of 2–8 during 2 h of incubation, showing higher activity at pH 6. Serine proteases and α-amylase inactivated significantly the antimicrobial activity of AS13, suggesting that the active molecule could most likely be a glycoprotein. These products are interesting for their possible application as biopreservatives in the food industry.

show abstract

Succession of bacterial microbiota in tilapia fillets at 4 °C and in situ investigation of spoilers

Cited by 14 publications

References 27 publications

Characterization of Bacterial Microbiota in Tilapia Fillets Under Different Storage Temperatures

Characterization of Bacterial Microbiota in Tilapia Fillets Under Different Storage Temperatures

Untitled

Assessing the inhibitory activity of culture supernatants against foodborne pathogens of two psychrotrophic bacteria isolated from river trout

Contact Info

Product

Resources

About