Successive determination of urinary protein and glucose using spectrophotometric sequential injection method

Watla‐iad, Kanchana; Sakai, Tadao; Teshima, Norio; Katoh, Shuji; Grudpan, Kate

doi:10.1016/j.aca.2007.10.010

Cited by 49 publications

(14 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Comparing the result with literature values shows that glucose detection limit obtained from our method is 3.08 µg mL -1 , Which shows 2.27 to 48.7 times sensitivity then earlier reported values i.e. 0.7 mg dL -1 , [37] 9 mg dL -1 [38] and 9-15 mg dL -1 [39].…”

Section: Comparison With the Literaturesupporting

confidence: 52%

Development of microwave assisted spectrophotometric method for the determination of glucose

Ali

Hussain

Arain

et al. 2016

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

View full text Add to dashboard Cite

Section: Comparison With the Literaturesupporting

confidence: 52%

Development of microwave assisted spectrophotometric method for the determination of glucose

Ali

Hussain

Arain

et al. 2016

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

View full text Add to dashboard Cite

“…16 Urinary protein level was determined using a spectrophotometric sequential injection method. 17 Creatinine clearance rate was calculated using the following formula:…”

Section: Renal Function Analysismentioning

confidence: 99%

Prenatal exposure to lipopolysaccharide alters the intrarenal renin–angiotensin system and renal damage in offspring rats

et al. 2009

View full text Add to dashboard Cite

Prenatal exposure to inflammation produces offspring that are hypertensive in adulthood. This study explored alterations of the renin-angiotensin system (RAS) during the development of hypertension induced by prenatal exposure to lipopolysaccharide (LPS). In addition, the effects of an inhibitor of the nuclear transcription factor (NF)-jB (pyrrolidine dithiocarbamate, PDTC) on this process were assessed. Pregnant rats were randomly divided into four groups (n¼8): a control group, an LPS group, a PDTC group and an LPS+PDTC group. The rats in these groups were intraperitoneally administered vehicle, 0.79 mg kg À1 LPS, 100 mg kg À1 PDTC or LPS plus PDTC, respectively. LPS was given on the 8th, 10th and 12th days, whereas PDTC was given from the 8th to the 14th day during gestation. At various ages from day 1 to 25 weeks, plasma renin activity, plasma angiotensin II (Ang II) levels, renal function, glomerular number, mRNA expression levels of renal cortex renin and angiotensin-converting enzyme (ACE), the number of Ang II-positive cells and NF-jB activation were determined. The results showed that prenatal exposure to LPS resulted in significantly lower glomerular numbers and creatinine clearance rates and higher urinary protein and renal cortex ACE mRNA expression in adult offspring. Prenatal LPS also decreased the renal cortex renin mRNA expression and the number of Ang II-positive cells in offspring at 1 day of age, but these increased at 7, 16 and 25 weeks, whereas the plasma renin activity and Ang II concentration remained unchanged. Simultaneously, PDTC treatment markedly reversed the action of LPS. In conclusion, prenatal exposure to LPS resulted in alteration of the intrarenal RAS and renal damage in adult offspring rats. INTRODUCTIONThe effects of hypertension on the cardiovascular and renal systems are of major concern due to its morbidity and mortality. Though many efforts have been made to understand the pathophysiology of hypertension, it is still unclear because of its complexity. 1 A growing body of evidence indicates that hypertension is an inflammatory state wherein proinflammatory cytokines, such as tumor necrosis factor-a and interleukin-6, contribute to the hypertensive effect. 2-5 Angiotensin II (Ang II), the major biologically active component of the reninangiotensin system (RAS), not only induces vasoconstriction, aldosterone release and sodium reabsorption by the nephron, but is also intricately interrelated with inflammation. Furthermore, the association of Ang II and inflammation induces an amplification process that involves oxidative stress and proinflammatory transcription factors, leading to progressive vascular injury 6 and having an important role in the development of hypertension and target organ damage. 2 It has been shown in our laboratory that maternal exposure to lipopolysaccharide (LPS) results in hypertension in offspring rats. 7

show abstract

“…Due to the fact that the SPV might induce the orientation of antibody on the surface of the electrode during the immobilization process, improved antigen binding capacity is anticipated comparing with randomly immobilized antibody by using glutaraldehyde as a conventional cross‐linker 26. Furthermore, our impedance‐based sensor showed lower LOD than amperometric biosensor (1 µg mL −1 ) 32. Compared to amperometry requiring a wide potential range to obtain a current signal, the impedance measurement is performed in a range of frequencies, using alternating current of a very narrow range of small potentials (less than 10 mV).…”

Section: Resultsmentioning

confidence: 97%

Label‐Free and Regenerative Electrochemical Microfluidic Biosensors for Continual Monitoring of Cell Secretomes

et al. 2017

View full text Add to dashboard Cite

Development of an efficient sensing platform capable of continual monitoring of biomarkers is needed to assess the functionality of the in vitro organoids and to evaluate their biological responses toward pharmaceutical compounds or chemical species over extended periods of time. Here, a novel label‐free microfluidic electrochemical (EC) biosensor with a unique built‐in on‐chip regeneration capability for continual measurement of cell‐secreted soluble biomarkers from an organoid culture in a fully automated manner without attenuating the sensor sensitivity is reported. The microfluidic EC biosensors are integrated with a human liver‐on‐a‐chip platform for continual monitoring of the metabolic activity of the organoids by measuring the levels of secreted biomarkers for up to 7 d, where the metabolic activity of the organoids is altered by a systemically applied drug. The variations in the biomarker levels are successfully measured by the microfluidic regenerative EC biosensors and agree well with cellular viability and enzyme‐linked immunosorbent assay analyses, validating the accuracy of the unique sensing platform. It is believed that this versatile and robust microfluidic EC biosensor that is capable of automated and continual detection of soluble biomarkers will find widespread use for long‐term monitoring of human organoids during drug toxicity studies or efficacy assessments of in vitro platforms.

show abstract

Successive determination of urinary protein and glucose using spectrophotometric sequential injection method

Cited by 49 publications

References 27 publications

Development of microwave assisted spectrophotometric method for the determination of glucose

Development of microwave assisted spectrophotometric method for the determination of glucose

Prenatal exposure to lipopolysaccharide alters the intrarenal renin–angiotensin system and renal damage in offspring rats

Label‐Free and Regenerative Electrochemical Microfluidic Biosensors for Continual Monitoring of Cell Secretomes

Contact Info

Product

Resources

About