Succinate dehydrogenase gene arrangement and expression in Anaplasma phagocytophilum

Massung, Robert F.; Hiratzka, Shannon L.; Brayton, Kelly A.; Palmer, Guy H.; Lee, Kemba N.

doi:10.1016/j.gene.2008.02.005

Cited by 5 publications

(2 citation statements)

References 36 publications

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“…Similar to the other Anaplasmataceae sequenced, the 5S and 23S rRNA genes form an operon (17), while the 16S rRNA gene is separated by ∼0.7 Mb and followed by sdh CD, as in A. phagocytophilum (25). Thirty-three tRNA genes are identified, which include cognates for all 20 amino acids.…”

Section: Resultsmentioning

confidence: 95%

Analysis of complete genome sequence of Neorickettsia risticii: causative agent of Potomac horse fever

Lin

Zhang

Gibson

et al. 2009

Nucleic Acids Research

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Neorickettsia risticii is an obligate intracellular bacterium of the trematodes and mammals. Horses develop Potomac horse fever (PHF) when they ingest aquatic insects containing encysted N. risticii-infected trematodes. The complete genome sequence of N. risticii Illinois consists of a single circular chromosome of 879 977 bp and encodes 38 RNA species and 898 proteins. Although N. risticii has limited ability to synthesize amino acids and lacks many metabolic pathways, it is capable of making major vitamins, cofactors and nucleotides. Comparison with its closely related human pathogen N. sennetsu showed that 758 (88.2%) of protein-coding genes are conserved between N. risticii and N. sennetsu. Four-way comparison of genes among N. risticii and other Anaplasmataceae showed that most genes are either shared among Anaplasmataceae (525 orthologs that generally associated with housekeeping functions), or specific to each genome (>200 genes that are mostly hypothetical proteins). Genes potentially involved in the pathogenesis of N. risticii were identified, including those encoding putative outer membrane proteins, two-component systems and a type IV secretion system (T4SS). The bipolar localization of T4SS pilus protein VirB2 on the bacterial surface was demonstrated for the first time in obligate intracellular bacteria. These data provide insights toward genomic potential of N. risticii and intracellular parasitism, and facilitate our understanding of PHF pathogenesis.

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Section: Resultsmentioning

confidence: 95%

Analysis of complete genome sequence of Neorickettsia risticii: causative agent of Potomac horse fever

Lin

Zhang

Gibson

et al. 2009

Nucleic Acids Research

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“…However, at present we cannot rule out other rearrangement generating processes like recombination. The succinate dehydrogenase gene arrangement and expression has been recently studied in Anaplasma phagocytophilum , another genome-reduced bacterium [64]. In that work, the authors described an overall conservation of sdh genes and critical amino acids, suggesting that these subunits remain functional.…”

Section: Discussionmentioning

confidence: 99%

The Twin-Arginine Translocation Pathway in α-Proteobacteria Is Functionally Preserved Irrespective of Genomic and Regulatory Divergence

2012

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The twin-arginine translocation (Tat) pathway exports fully folded proteins out of the cytoplasm of Gram-negative and Gram-positive bacteria. Although much progress has been made in unraveling the molecular mechanism and biochemical characterization of the Tat system, little is known concerning its functionality and biological role to confer adaptive skills, symbiosis or pathogenesis in the α-proteobacteria class. A comparative genomic analysis in the α-proteobacteria class confirmed the presence of tatA , tatB , and tatC genes in almost all genomes, but significant variations in gene synteny and rearrangements were found in the order Rickettsiales with respect to the typically described operon organization. Transcription of tat genes was confirmed for Anaplasma marginale str. St. Maries and Brucella abortus 2308, two α-proteobacteria with full and partial intracellular lifestyles, respectively. The tat genes of A. marginale are scattered throughout the genome, in contrast to the more generalized operon organization. Particularly, tatA showed an approximately 20-fold increase in mRNA levels relative to tatB and tatC . We showed Tat functionality in B. abortus 2308 for the first time, and confirmed conservation of functionality in A. marginale . We present the first experimental description of the Tat system in the Anaplasmataceae and Brucellaceae families. In particular, in A. marginale Tat functionality is conserved despite operon splitting as a consequence of genome rearrangements. Further studies will be required to understand how the proper stoichiometry of the Tat protein complex and its biological role are achieved. In addition, the predicted substrates might be the evidence of role of the Tat translocation system in the transition process from a free-living to a parasitic lifestyle in these α-proteobacteria.

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Isolation, structure determination and antibacterial activities of succinamide conjugate diacid from Acinetobacter sp. BJ-L

Dengfeng

Chang

Wang

et al. 2011

Microbiological Research

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Strain BJ-L was isolated from the a water sample taken from Xiao Yue River in Beijing and identified as Acinetobacter sp. BJ-L based on the study of its morphology, physiology, biochemistry and 16S rRNA gene sequence. A new antimicrobial substance was produced after the strain was incubated in potato extract medium at 15°C for 72 h. The antimicrobial substance was sequentially purified by reduced pressure condensation, EtOAc extract, and silica gel column chromatography. The structure of the antimicrobial substance was elucidated as succinamide conjugate diacid (SCD) by spectroscopic data interpretation. Structure analysis indicated that SCD is a novel compound and that it could inhibit the growth of some tested bacterial strains with the MIC of 2mg/ml, such as Staphylococcus aureus, Bacillus subtilis, Enterobacter aerogenes and Escherichia coli. Moreover, no obvious toxicity has been found on cultured HUVEC cells with different concentrations of SCD at 5, 10, 15, and 20mg/ml.

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Succinate dehydrogenase gene arrangement and expression in Anaplasma phagocytophilum

Cited by 5 publications

References 36 publications

Analysis of complete genome sequence of Neorickettsia risticii: causative agent of Potomac horse fever

Analysis of complete genome sequence of Neorickettsia risticii: causative agent of Potomac horse fever

The Twin-Arginine Translocation Pathway in α-Proteobacteria Is Functionally Preserved Irrespective of Genomic and Regulatory Divergence

Isolation, structure determination and antibacterial activities of succinamide conjugate diacid from Acinetobacter sp. BJ-L

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