Sucrose regulated expression of a Ca2+-dependent protein kinase (TaCDPK1) gene in excised leaves of wheat

Martínez-Noël, Giselle; Nagaraj, Vinay J.; Caló, Gonzalo; Wiemken, Andres; Pontis, Horacio G.

doi:10.1016/j.plaphy.2007.03.004

Cited by 29 publications

(16 citation statements)

References 39 publications

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“…During this project, nine accessions of wheat CDPK genes appeared in GenBank that are either identical or putative homoeologs of TaCPK1 (ABK23687), TaCPK2 (AY704444), TaCPK4 (DQ333376, DQ333377), TaCPK5 (DQ333374, DQ431850, DQ333375, DQ431851) and TaCPK8 (AJ621356) ( Table 1). Recently, one wheat CDPK (AJ621356, identical to TaCPK8 in this study) is reported by Martínez-Noël et al (2007) and shown to be involved in sucrose induced signaling pathway in excised wheat leaves. Its role in stress responses, however, was not studied.…”

Section: Isolation Of Wheat Cdpk Genesmentioning

confidence: 66%

“…Systematic analysis of wheat CDPK genes has not been reported, although a few CDPK gene sequences have been deposited in GenBank. Recently, Martínez-Noël et al (2007) isolated a wheat CDPK (corresponding to TaCPK8 in this study) and studied its regulation by sucrose using excised leaves. We analyzed the expression patterns of all 20 CDPKs under various stress conditions.…”

Section: Differential Expression Of Cdpk Genes In a Polyploid Speciesmentioning

confidence: 99%

“…CDPKs have been demonstrated to be involved in regulating plant responses to a variety of stimuli, including hormones, cold/drought/salt stress, elicitor, light and sucrose (Urao et al 1994;Botella et al 1996;Sheen 1996;Frattini et al 1999;Patharkar and Cushman 2000;Saijo et al 2000;Romeis et al 2001;Murillo et al 2001;Yang and Komatsu 2001;Chico et al 2002;Martínez-Noël et al 2007;Tsai et al 2007;Wan et al 2007). In rice, transgenic plants with a disrupted SPK (a seed-specific CDPK) exhibited reduced accumulation of storage starch and proteins in immature seeds (Asano et al 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Till now, only one wheat CDPK gene TaCDPK1 was reported to be involved in sucrose induced signaling pathways in wheat leaves (Martínez-Noël et al 2007). Moreover, most CDPK genes of wheat have not been clearly correlated with responses to specific biotic and abiotic stresses.…”

Section: Introductionmentioning

confidence: 99%

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Evolutionary and functional study of the CDPK gene family in wheat (Triticum aestivum L.)

et al. 2008

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Calcium-dependent protein kinases (CDPKs) are crucial sensors of calcium concentration changes in plant cells under diverse endogenous and environmental stimuli. We identified 20 CDPK genes from bread wheat and performed a comprehensive study on their structural, functional and evolutionary characteristics. Full-length cDNA sequences of 14 CDPKs were obtained using various approaches. Wheat CDPKs were found to be similar to their counterparts in rice in genomic structure, GC content, subcellular localization, and subgroup classification. Divergence time estimation of wheat CDPK gene pairs and wheat-rice orthologs suggested that most duplicated genes already existed in the common ancestor of wheat and rice. The number of CDPKs in diploid wheat genome was estimated to be at least 26, a number close to that in rice, Arabidopsis, and poplar. However, polymorphism among EST sequences uncovered transcripts of all three homoeologous alleles for 13 out of 20 CDPKs. Thus, the hexaploid wheat should have 2-3 fold more CDPK genes expressing in their cells than the diploid species. Wheat CDPK genes were found to respond to various biotic and abiotic stimuli, including cold, hydrogen peroxide (H(2)O(2)), salt, drought, powdery mildew (Blumeria graminis tritici, Bgt), as well as phytohormones abscisic acid (ABA) and gibberellic acid (GA). Each CDPK gene often responded to multiple treatments, suggesting that wheat CDPKs are converging points for multiple signal transduction pathways. The current work represents the first comprehensive study of CDPK genes in bread wheat and provides a foundation for further functional study of this important gene family in Triticeae.

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Section: Isolation Of Wheat Cdpk Genesmentioning

confidence: 66%

Section: Differential Expression Of Cdpk Genes In a Polyploid Speciesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Evolutionary and functional study of the CDPK gene family in wheat (Triticum aestivum L.)

et al. 2008

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“…Other signaling intermediates, such as Ca 2? , calmodulin, protein kinases, and protein phosphatases etc., are concerned with sugar signaling pathway (Martínez-Noël et al 2007;Vitrac et al 2000). The effect of sugars on genes involved in anthocyanin biosynthesis and the potential involvement of hexokinase have already been studied previously in other plant systems including grape cell suspensions (Neta-Sharir et al 2000;Vitrac et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Sugars induce anthocyanin accumulation and flavanone 3-hydroxylase expression in grape berries

et al. 2009

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Flavanone 3-hydroxylase (EC 1.14.11.9, F3H) plays a key role in anthocyanin biosynthesis, and sugars enhance anthocyanin accumulation and F3H expression in some other plants. However, information about the relationship between sugars, anthocyanin accumulation and F3H expression in grape berries has been little reported. Present experiment was done with sliced grape berry system. The optimum fruit developmental stage, sugar concentration, and incubation time in sugar induction anthocyanin accumulation and F3H expression were determined. Mannose and 2-deoxyglucose, glucose analogs known to be phosphorylated by hexokinase but are poorly metabolized, obviously induced the anthocyanin accumulation and F3H expression, whereas 3-O-methylglucose and 6-deoxyglucose, glucose analogs transported inside the cell but not substrates for hexokinase, did not induce them. Glucosamine and mannoheptulose, the specific inhibitors of hexokinase, blocked the activation induced by sugar on both anthocyanin accumulation and F3H expression.

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Genomics of Tolerance to Abiotic Stress in the Triticeae

Maccaferri¹,

Sanguineti

Giuliani

et al. 2009

Genetics and Genomics of the Triticeae

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Sucrose regulated expression of a Ca2+-dependent protein kinase (TaCDPK1) gene in excised leaves of wheat

Cited by 29 publications

References 39 publications

Evolutionary and functional study of the CDPK gene family in wheat (Triticum aestivum L.)

Evolutionary and functional study of the CDPK gene family in wheat (Triticum aestivum L.)

Sugars induce anthocyanin accumulation and flavanone 3-hydroxylase expression in grape berries

Genomics of Tolerance to Abiotic Stress in the Triticeae

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