Sugar Beet Micropropagation

Mezei, S.; Kovačev, Lazar; Nagl, Nevena

doi:10.1080/13102818.2006.10817296

Cited by 12 publications

(8 citation statements)

References 27 publications

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“…In some cases they do not use the full concentration of salts, provided by the protocol, but only 0.25-0.50 of their content while preserving the correlation which concerns primarily the rhizogenesis process. High salt concentration delayed the roots formation in vitro, which caused the need to reduce their concentration (Mezei et al, 2006;Kosenkoet al, 2008).…”

Section: Resultsmentioning

confidence: 99%

“…The described changes can be both hereditary and non-hereditary (modification). Violation of correlative relationships and the fact that in vitro cell division occurs without genetically predetermined organism control that is carried out by endogenous phytohormones in an integrated plant account for somaclonal variability (Mezei et al, 2006;Penna et al, 2012). Chromosome variability of cells in vitro is among the best studied cytological disorders, like endomitosis and endoreduplication whicht causes polytene chromosomes formation, which are formed due to repeated DNA synthesis without further nucleus and cells fission (Carvalheira, 2000).…”

Section: Variant 1 Control (No Dye)mentioning

confidence: 99%

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In vitro rhizogenesis of sugar beet microclones

Полищук

Каrpuk

Mykolaiko

et al. 2017

Regul. Mech. Biosyst.

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The features of rhizogenesis of male-sterile simple hybrids, maternal components of sugar beet heterozosis hybrid and O-types of Darinka variety grown from seeds were investigated. The paper presents the results of studying the main basic nutrient media (B5–A1, B5–A2, B5–A3, B5–A4, B5–A5 and B5–A6) for rhizogenesis, in which the content of macro- and microelements has been reduced by three times, the content of sucrose is reduced by almost 6 times, the amount of agar is reduced by almost five times, while the vitamin РР content is increased by 1.6 times, and the BAP is completely removed. The largest number of rooted microclones was obtained with NAAs by the high indexes of average number of roots and medium indexes of average roots length. To the second, by the number of rooted microclones is assigned, media of 2,4–D, 2,4–DB containing, potassium salt of NAA and IBA were classified, while IAA was characterized by a lower efficiency. The possibilities of regulation of growth and development processes of sugar beet in vitro explants in favor of undifferentiated growth at the stage of proliferation or organogenesis (hemo- and rhizogenesis) have been determined. To improve the quality of rooting of sugar beet plants-regenerants, we have developed the method for dimming agarized nutrient medium with methylene blue at a 0.05% concentration, which made it possible to reduce the inhibitory effect of light on the development of lateral roots. The average index of micro-roots rooting for MS-forms was 81.5 %. The technology of adaptation of micro-roots rooting was developed, in which the plant material at the initial stage of growth had slight morphological changes in the puffer apparatus and stem, but by the end of the vegetation, the plants acquired the appearance characteristic of the donor plants of explants. The clone’s resiliency is reached about 100% in the soil. According to the variability of morphological characteristics in reproduced in vitro sugar beet plants of the MS-forms of the Darynka hybrid, all the plants grown from the regenerants were more, than seed progeny from seed sowing of the same component obtained as a result of the attachment of sterility to the corresponding O-type. It has been found that in vitro cultivation has not weakened, and in some cases even increased the mitotic index of meristem cells of young roots of sugar beet. Most genotypes have reduced the number of pathological mitoses, apparently due to the activation of reparative systems, but did not extend the duration of individual phases of mitosis; without increasing the number of anaphase with bridges and fragments, but changed the number of anaphases with other chromosomal abnormalities. In all variants of the experiment, where activation of growth processes has been observed, the lowest variability of the signs is observed, that is, under the influence of stimulants, which are part of nutrient media, obviously there is unification of cell populations.

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Section: Resultsmentioning

confidence: 99%

Section: Variant 1 Control (No Dye)mentioning

confidence: 99%

In vitro rhizogenesis of sugar beet microclones

Полищук

Каrpuk

Mykolaiko

et al. 2017

Regul. Mech. Biosyst.

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“…Seeds were surface sterilized and placed on MS medium (Murashige and Skoog, 1962) with 30 g/l sucrose and 7 g/l agar, for germination. After 10 days, the seedlings were transferred onto micropropagation medium: MS medium containing 0.01 mg/l Gibberellic acid (GA3) and 0.3 mg/l 6-Benzylaminopurine (BAP), pH 5.8 (Mezei et al 2006). They were multiplied for twelve weeks, with subcultivations every three weeks, and then transferred onto micropropagation media containing 0%, 3% and 5% (w/v) polyethylene glycol (PEG 6000) in order to induce water stress.…”

Section: Plant Materials and Experimental Treatmentmentioning

confidence: 99%

The use of rice straw broth as an appropriate medium to isolate purple nonsulfur bacteria from paddy fields

Taški-Ajduković

Nagl

Kovačev

et al. 2012

Electron. J. Biotechnol.

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Sugar beet is a significant industrial crop, often grown in the areas where summer drought can severely limit root yield and sugar content. In order to improve development of sugar beet cultivars with increased drought tolerance it is necessary to understand plant response to water stress at the genomic level. Since recent research efforts have focused on the molecular response of the plant in order to identify water deficit inducible genes, the aim of this investigation was to develop qRT-PCR methodology for the quantification of gene expression in sugar beet under conditions of water deficiency in vitro. Sugar beet genotypes, selected for different response to water deficit, were grown and multiplied in vitro. Axilary shoots were placed on micropropagation media with 0%, 3% and 5% PEG, for 28 days. To determine reaction of sugar beet genotypes to in vitro induced water deficit changes in number of axillary shoots, shoot fresh weight and dry matter content were measured. Total RNA was extracted from leaves and reverse transcribed into cDNA, which served as matrix in real-time PCR reaction using TaqMan technology. The housekeeping gene for glutamine synthetase was used as endogenous control, while the genes for alpha amylase and osmotin-like protein were target genes. The relative quantification values for each target gene were calculated by the 2 −ΔΔCt method. Selected candidate genes differed in relative gene expression among genotypes and applied PEG treatments. The obtained results indicated that qRT-PCR protocol was efficient and accurate, showing the potential to be used in further expression analysis of candidate genes involved in sugar beet reaction to water stress.How to reference this article: TASKI-AJDUKOVIC, K.; CURCIC, Z. and DANOJEVIC, D. (2012). Development and application of qRT-PCR for sugarbeet gene expression analysis in response to in vitro induced water deficit.

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“…Generally, biotechnological techniques require in vitro methods to provide starting material for research and breeding. Sugar beet breeding has benefitted from several tissue culture techniques (Mezei et al, 2006). Despite this fact, in vitro techniques for sugar beet still lag behind those for many major crops (Maluszynski et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

The effects of proline on in vitro proliferation and propagation of doubled haploid sugar beet (Beta vulgaris)

2018

Turk J Bot

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Doubled haploid induction is one of the available methods normally used for sugar beet breeding. Gynogenic haploid explants of sugar beet induced with 1 or 2 mg L -1 6-benzylaminopurine were treated with 5 g L -1 colchicine, then subcultured on a solidified MS medium plus 0.2 mg L -1 kinetin. Colchicine doubled the chromosome number of 27.7% of the treated haploid explants. With the aim of increasing the number of doubled haploid explants, the effects of five levels of proline (0.0, 0.1, 0.2, 0.3, or 0.4 mM) on the explants' proliferation, propagation, and shoot length were compared. With a large effect size (ES), proline at 0.3 mM induced the highest amount of proliferation, while proline-free medium resulted in the lowest amount of it. The highest propagation rates were observed for the explants treated on media with 0.2 and 0.3 mM proline (very large ES). Proline at 0.3 mM induced the shortest shoots (medium ES). A very strong positive correlation between proliferation and propagation, a moderate negative correlation between proliferation and length, and a strong negative correlation between propagation and length were observed. For the first time our results show beneficial effects of proline on in vitro proliferation and propagation of sugar beet.

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Sugar Beet Micropropagation

Cited by 12 publications

References 27 publications

In vitro rhizogenesis of sugar beet microclones

In vitro rhizogenesis of sugar beet microclones

The use of rice straw broth as an appropriate medium to isolate purple nonsulfur bacteria from paddy fields

The effects of proline on in vitro proliferation and propagation of doubled haploid sugar beet (Beta vulgaris)

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