Vegetative in vitro multiplication is one of the most efficient methods for sugar beet (Beta vulgaris L.) propagation. The usual steps in this procedure are sterilization of explant, multiplication, rhizogenesis and acclimatization. In the paper is presented development of regeneration and multiplication techniques from different explants. It gives a detailed description of further micropropagation steps and presents necessary conditions for their realization. The paper also discuss different ways of micropropagation application especially in sugar beet breeding, but in other plant sciences as well.

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The aim of research was obtaining sugar beet haploids via gyno-genesis and their micropropagation. Haploids were obtained by ovule culture from fourteen diploid, monogerm, fertile genotypes. On the tested nutrient media genotypes exhibited different gynogenic potential. Eight haploid plant were chosen for further investigation and after development of first leaves put on micropropagation medium. The presence of cyto-kinin in medium stimulated development of axillary buds, while in some genotypes adventitious buds developed as well. Multiplication rate was not consistent, although number of developed plants grew after each sub-cultivation. Differences in plant multiplication started to differ after four subcultures. By testing of differences between correlation coefficients, i.e. multiplication rate during six subcultivations, it was determined that they significantly differ between tested genotypes

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