Sugarcane Juice as an Alternative Carbon Source for in vitro Culture of Plantains and Bananas

Buah, J. N.; Tachie-Men, J.W.; Addae, G.; Asare, Paul A.

doi:10.3923/ajft.2011.685.694

Cited by 13 publications

(8 citation statements)

References 9 publications

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“…For the plant to survive, it needs energy and the osmotic potential needs a carbohydrate source in the culture media itself (Yaseen et al 2013). Water movement and potential and mineral movements in the media are the most important factors that are affected by carbon sources (Buah et al 2011). Based on plants spices' genotype and explants, many carbon sources have been used to develop somatic embryogenesis (Buah et al 2011;Yaseen et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Water movement and potential and mineral movements in the media are the most important factors that are affected by carbon sources (Buah et al 2011). Based on plants spices' genotype and explants, many carbon sources have been used to develop somatic embryogenesis (Buah et al 2011;Yaseen et al 2013). Growth, development and morphogenesis of plant organs, tissues and cells in vitro culture are importantly affected by the composition of mineral nutrients (Sivanesan and Park 2014).…”

Section: Introductionmentioning

confidence: 99%

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Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol

Abiri

Mahmood

Shaharuddin

et al. 2017

Int. J. Environ. Sci. Technol.

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Enhancing of the efficient tissue culture protocol for somatic embryos would facilitate the engineered breeding plants program. In this report, we describe the reproducible protocol of Malaysian rice (Oryza sativa L.) cultivar MR219 through somatic embryogenesis. Effect of a wide spectrum of exogenesis materials was assessed in three phases, namely callogenesis, proliferation and regeneration. Initially, rice seeds were subjected under various auxin treatments. Secondly, the effect of different concentrations of 2,4-D on callus induction was evaluated. In the next step, the efficiency of different explants was identified. Subsequently, the effects of different auxins, cytokinins, L-proline, casein hydrolysate and potassium metasilicate concentrations on the callus proliferation and regeneration were considered. For the callogenesis phase, 2 mg L -1 of 2,4-D and roots were chosen as the best auxin and explant. In the callus proliferation stage, the highest efficiency was observed at week eight in the MS media supplemented with 2 mg

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol

Abiri

Mahmood

Shaharuddin

et al. 2017

Int. J. Environ. Sci. Technol.

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“…Santana et al (2009) utilizaram melado de cana-de-açúcar, como fonte de carboidrato e vitaminas, para o cultivo in vitro de mandioca, e observaram que os números médios de folhas e de raízes e a biomassa das matérias seca e fresca das plantas cultivadas em meio com melado de cana-de-açúcar não diferiram daqueles do grupo controle, corroborando os resultados obtidos neste trabalho. Buah et al, (2011) estudaram o efeito do caldo de cana-de-açúcar sobre o desenvolvimento in vitro de bananeiras e observaram que os maiores valores de núme-ro médio de brotos e de biomassa fresca ocorreram nos tratamentos com sacarose e com 5% de caldo de canade-açúcar. Esses resultados estão de acordo com aqueles obtidos neste estudo.…”

Section: Resultsunclassified

“…Entretanto, para a produção comercial de plantas em laboratórios, esse grau de pureza não é necessário (Prakash et al 2004), desde que sejam adotadas técnicas eficientes de esterilização. Meios nutritivos alternativos, preparados a partir de derivados do processamento da cana-de-açúcar, já foram descritos na literatura, apresentando resultados satisfatórios para o cultivo in vitro de diferentes espécies de plantas (Dhamankar, 1992;Santana et al, 2009;Buah et al, 2011;Ribeiro et al, 2012).…”

Section: Introductionunclassified

Uso da rapadura como meio nutritivo para cultivo in vitro de bananeira cv. Maçã

et al. 2013

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Use of panela as nutritive medium for in vitro culture of banana cv. MaçãThe purpose of this study was to evaluate the effect of panela (hard brown sugar) on in vitro development of banana cv. Maçã, in order to reduce production costs. Banana explants were inoculated in nutrient media formulated with four different concentrations of panela solution (10, 25, 50 and 75%) and data were compared with those of plants grown on MS medium (control), totalizing five treatments. After 60 days, the average number of leaves, shoots and roots, as well as the mean number of explants dead and oxidized were analyzed. There was no significant difference between treatments for most parameters, except for the mean number of oxidized explants, which was higher in the treatment containing 75% of panela. It was concluded that nutrient media with up to 50% of panela in the composition, without plant growth regulators, can be used for in vitro banana replacing the MS media.

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“…O menor desenvolvimento das plantas, observado ao se utilizar um derivado da cana-de-açúcar como fonte de nutrientes, também pode estar relacionado com o fato de esses produtos apresentarem fatores inibitórios do crescimento (Buah et al, 2011). Os autores citados, trabalhando com caldo de cana, um outro derivado de cana-deaçúcar, observaram que uma concentração mais elevada de caldo no meio de cultura inibia o desenvolvimento de explantes de bananeira, sendo o fator limitante para o crescimento das plantas.…”

Section: Resultsunclassified

Efeito do melado de cana-de-açúcar no desenvolvimento in vitro de bananeira (Musa spp.) cv. Maçã

et al. 2012

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Effect of sugarcane molasses on the in vitro development of banana (Musa spp.) cv. MaçãDespite offering a number of advantages as the quality of final product, the technique of in vitro cultivation of plants is still considered expensive due to, among other things, the use of reagents with high purity for the preparation of nutrient media. Among the alternatives that can be adopted to reduce the costs associated with the production of seedlings, the replacement of the PA product for those with lower has potential. Based on this information, the purpose of this study was to evaluate the effect of molasses of sugar cane on in vitro development of banana cv. Maçã. For this purpose, banana plants were inoculated in nutrient media made on the basis of sugarcane molasses, with four different concentrations (Brix of 1.5, 3.0, 4.5 and 6.0) and data were compared with those of plants grown on MS medium (control), in a total of five treatments. Despite plants have been developed in all treatments, it was observed that for the variables leaf number and fresh biomass, plants cultivated in MS medium (control) had higher performances. However, for the average number of roots, there was no statistical difference between treatments. The sugarcane molasses did not favor the development of banana plants, but can be used for in vitro rooting of these plants. Apesar de oferecer uma série de vantagens, quanto à qualidade do produto final, a técnica de cultivo in vitro de plantas ainda é considerada dispendiosa, por causa, dentre outros fatores, da utilização de reagentes com alto grau de pureza no preparo de meios nutritivos. Entre as alternativas que podem ser adotadas para a redução dos custos relacionados com a produção de mudas, apresenta potencial a substituição de produtos PA por aqueles de menor custo. Baseado nestas informações, o objetivo deste estudo foi avaliar o efeito do melado de cana-de-açúcar sobre o desenvolvimento in vitro de bananeira, cv. Maçã. Para tal propósito, plantas de bananeira foram inoculadas em meios nutritivos, formulados à base de melado de cana-de-açúcar, com quatro concentrações distintas (brix de 1,5; 3,0; 4,5 e 6,0), e os dados obtidos foram comparados com aqueles das plantas cultivadas em meio MS (controle), perfazendo um total de cinco tratamentos. Apesar de terem-se desenvolvido em todos os tratamentos testados, observou-se que, para as variáveis número médio de folhas e biomassa da matéria fresca, as plantas cultivadas no meio MS (controle) apresentaram valores superiores. Entretanto, para o número médio de raízes, não houve diferença estatística entre os tratamentos utilizados. O melado de cana-de-açúcar não favorece o desenvolvimento de plântulas de bananeira, mas pode ser utilizado para o enraizamento dessas plantas in vitro.Palavras-chave: redução de custos, micropropagação, reagente comercial.

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Sugarcane Juice as an Alternative Carbon Source for in vitro Culture of Plantains and Bananas

Cited by 13 publications

References 9 publications

Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol

Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol

Uso da rapadura como meio nutritivo para cultivo in vitro de bananeira cv. Maçã

Efeito do melado de cana-de-açúcar no desenvolvimento in vitro de bananeira (Musa spp.) cv. Maçã

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