Sugarcane molasses and vinasse as a substrate for polyhydroxyalkanoates (PHA) production

Cárdenas, Alejandro Acosta; Zapata, Wilman Alcaraz; Cardona-Betancur, Mariana

doi:10.15446/dyna.v85n206.68279

Cited by 30 publications

(13 citation statements)

References 16 publications

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“…The realistic scenarios had total process times consistent with those experimentally described by other authors (Acosta-Cárdenas et al, 2018;Bormann & Roth, 1999;Ienczak et al, 2011;Kachrimanidou et al, 2014;Rodríguez-Contreras et al, 2015). These authors described bioprocesses with R. eutropha strains that used glycerol as carbon source and fermentation times ranged from 45 h to 60 h. Thus, high cell density bioprocess with duration times below 45 h could indeed be considered optimistic scenarios.…”

Section: Leong Et Al (2017)supporting

confidence: 68%

Technical-Economical Approach for PHB Production by Ralstonia Eutropha Strain Using Concentrated Vinasse as Carbon Source and Other Biotechnological Applications

Silverio

Piccoli

Reis

et al. 2021

Preprint

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The Brazilian ethanol industry is one of the most important in the global market, however these important industrial activities have been generating significant amounts of vinasse and its management has become costly for distilleries. In this study, the aim was to evaluate concentrated and in natura vinasse as basal culture media for biotechnological processes. Different bacteria and processes were assessed: L-threonine production by E. coli THR14, with glucose as carbon source; PHB production by halophilic strain Halomonas sp. HG03, with sucrose as carbon source; and PHB biosynthesis by R. eutropha L359PCJ, which used glycerol from vinasse as carbon source. Strains were evaluated firstly in shake flasks cultivations using vinasse-based media. E. coli THR14 had no statistical difference for biomass and L-threonine concentrations among control and vinasse-based treatments (up to 50% v v-1 of in natura vinasse). Halomonas sp. HG03 and R. eutropha L359PCJ were cultivated in mineral media diluted by in natura (50% and 75% v v-1) and concentrated (50% and 75% v v-1) vinasses. Higher vinasse concentrations resulted in higher cellular growth rather than PHB accumulation for both bacteria. In vinasse-based treatments, Halomonas sp. HG03 had PHB content between 19.6 – 75.2% and R. eutropha L359PCJ, 48.4 – 68.5%. 50% (v v-1) of concentrated vinasse was the most attractive condition for PHB production by both bacteria. Further experiments in CSTR bioreactors used this nutritional condition and R. eutropha L359PCJ had PHB content of 66.3%, concentrations of residual cell dry weight (rCDW) = 9.4 g L-1 and PHB = 18.6 g L-1, with YX/S = 0.16 g gGLYCEROL-1, YP/S = 0.32 g gGLYCEROL-1 and 0.25 gPHB Lh-1. Halomonas sp. HG03 had PHB content of 45.7%, rCDW = 9.8 g L-1, PHB = 8.3 g L-1 and YX/S = 0.18 g gSUCROSE-1, YP/S = 0.16 g gSUCROSE-1 and 0.12 gPHB Lh-1. Finally, cost reductions of PHB production by R. eutropha L359PCJ with concentrated vinasse-based medium were evaluated in silico by using SuperPro Designer. As a partial source of glycerol and other nutrients for PHB production by R. eutropha L359PCJ, vinasse reduced overall production costs by 13%. Simulated processes that used concentrated vinasse-based media combined with improvements of PHB productivity and higher cellular densities had production costs between US$ 3.9 – 7.5/kgPHB and 2.6 – 7.3 years of payback time.

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Section: Leong Et Al (2017)supporting

confidence: 68%

Technical-Economical Approach for PHB Production by Ralstonia Eutropha Strain Using Concentrated Vinasse as Carbon Source and Other Biotechnological Applications

Silverio

Piccoli

Reis

et al. 2021

Preprint

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“…The producing PHB bacterial strain was isolated from a soil sample collected from the botanic garden of Skikda University by performing serial dilutions. Pure, viable colonies were then grown in a mineral salt medium (MSM) [20] to which 2% glucose and 10 g of agar were added; the mixture was then incubated for 48 h at 37 °C. The PHB-producing colonies were detected by adding an ethanolic solution of 3% Sudan Black B (C29H24N6 224-087, Sigma).…”

Section: Methodsmentioning

confidence: 99%

“…The PHB-producing colonies were detected by adding an ethanolic solution of 3% Sudan Black B (C29H24N6 224-087, Sigma). The Sudan Black B positive isolate was tested for its ability to produce PHB in the agar MSM medium [20] and added 2% of carob molasses instead of glucose. The bacterial strain was spotted onto the MSM agar medium and incubated at 37 °C for 48 h. The bacterial colonies were colored with an ethanolic solution of 3% Sudan Black B (C29H24N6 224-087, Sigma) to confirm its ability to produce PHB [21] .…”

Section: Methodsmentioning

confidence: 99%

Potential use of Bacillus paramycoides for the production of the biopolymer polyhydroxybutyrate from leftover carob fruit agro-waste

DJERRAB

CHEKROUD²,

ROUABHIA

et al. 2022

AIMSMICRO

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<abstract> This study was designed to investigate, at a laboratory scale, the possibility of valorizing the leftover carob fruits to produce the eco-friendly biopolymer polyhydroxybutyrate (PHB) by using the bacterial strain <italic>Bacillus paramycoides</italic>, which has been isolated from the botanical garden of Skikda University in Algeria. The PHB production was tested under various conditions: a pH of 3–8, temperature range of 30–44 °C, carob extracted molasses concentration of 2–8% v/v, an incubation time of 24–96 h and an agitation speed of 150–300 rpm. The effects of different nitrogen sources and carob extracted molasses treatment types were also investigated. The PHB concentration was determined quantitatively as crotonic acid by measuring the absorbance at 300 nm. Cell growth was quantified by measuring the density of the culture at 600 nm. The presence of PHB was confirmed by applying high-performance liquid chromatography (HPLC) using an Aminex HPX-87H and implementing gas chromatography analysis. The best yield of PHB synthesis was obtained by using 6% v/v of 5 M H2SO4 treated with carob molasses as a carbon source, with peptone as a nitrogen source; incubation was conducted at 37 °C for 96 h at an agitation speed of 300 rpm (114.95 mg/L). The HPLC analysis confirmed the synthesis of PHB by <italic>B. paramycoides</italic> to have a chromatogram retention time of 22.5 min. Carob waste was successfully valorized to PHB. </abstract>

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“…Sugarcane vinasses and molasses (in a 75%/25% vol. mixture, respectively) were used as culture medium, as reported by Acosta-Cárdenas et al 32 Pilot plant experiments were carried out in a 500 L bioreactor located at University of Antioquia (Colombia). All experiments started in a batch phase, followed by a fed-batch stage.…”

Section: Methodsmentioning

confidence: 99%

Real‐time optimization and control for polyhydroxybutyrate fed‐batch production at pilot plant scale

Ochoa

Alcaraz

2020

J of Chemical Tech & Biotech

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BACKGROUND: Polyhydroxyalkanoate-type polymers (PHAs) are claimed as an alternative for replacing conventional plastics; but the economic feasibility of its production should still be assured in order to accomplish such a massive goal. In a previous work on fed-batch production of PHAs, it was shown that the process yield and the properties of the biopolymer obtained highly depend on the type of feeding strategy applied. Such results evidenced the need for implementing optimization-based control strategies to control the process in real-time, rather than implementing pre-defined feeding policies. RESULTS: In this study, real-time optimization for the fed-batch production of PHAs at pilot plant scale from a low-cost substrate (vinasses-molasses mixture) is presented. Two optimization-based control strategies were developed to calculate the optimal feed flow rate. One strategy considered only the process productivity as an objective, whereas the other incorporated not only productivity but also a profitability-oriented objective. The latter resulted in a productivity of 0.364 g/L h and a maximal polymer concentration of 62.03% cell dry weight, versus 0.043 g/L h and 6.25% attained by applying a traditional feedback control law. CONCLUSION: Implementation of closed-loop based-optimal control policies in PHA production has led to higher yield and polymer concentration, confirming that an improved operation of fed-batch bioprocesses is reached when the feeding policy is updated in real-time, according to an optimization goal. Despite implementation costs, applying this approach might be the best alternative for simultaneously facing uncertainties and achieving optimal operation at an industrial scale, especially when living microorganisms are used as factories.

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Sugarcane molasses and vinasse as a substrate for polyhydroxyalkanoates (PHA) production

Cited by 30 publications

References 16 publications

Technical-Economical Approach for PHB Production by Ralstonia Eutropha Strain Using Concentrated Vinasse as Carbon Source and Other Biotechnological Applications

Technical-Economical Approach for PHB Production by Ralstonia Eutropha Strain Using Concentrated Vinasse as Carbon Source and Other Biotechnological Applications

Potential use of <i>Bacillus paramycoides</i> for the production of the biopolymer polyhydroxybutyrate from leftover carob fruit agro-waste

Real‐time optimization and control for polyhydroxybutyrate fed‐batch production at pilot plant scale

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