Sugarcane (Saccharum hybrid) Genetic Transformation Mediated by Agrobacterium tumefaciens: Production of Transgenic Plants Expressing Proteins with Agronomic and Industrial Value

Enríquez, G. A.; Trujillo, Luis E; Menéndez, Carmen; Vázquez, Roberto; Tiel, Kenia; Dafhnis, F.; Arrieta, Juan G.; Selman, G. G.; Hernández, Lázaro

doi:10.1016/s0168-7972(00)80011-0

Cited by 16 publications

(7 citation statements)

References 13 publications

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“…Agrobacterium tumefaciens-mediated gene transformation has been established both for dicot (Block et al, 1984) and monocot (Hiei, 1994) plants and is hence being used for different crops such as wheat (Jones, 2005), maize (Ahmadabadi, 2007), rice (Hiei et al, 1994), and sugarcane (Enriquez et al, 2000). Poor regeneration of transformants is still a major barrier in crop improvement through genetic engineering (Popelka and Altpeter, 2003).…”

Section: Introductionmentioning

confidence: 99%

Improving in vitro leaf disk regeneration system of sugarcane (Saccharum officinarum L.) with concurrent shoot/root induction from somatic embryos

Nawaz¹,

Ullah²,

Iqbal³

et al. 2013

Turk J Biol

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Genome engineering experiments are impeded by poor performance of regeneration systems. The present study was aimed at establishing a short and cost-effective in vitro regeneration system for elite sugarcane cultivars through simultaneous shoot/root induction. The innermost spindle leaf and shoot tip were used as explants. For callus induction, Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) was used and 5.0 mg/L of 2,4-D supported maximum callus induction (84.5%). Three-week-old calli were treated with different levels of benzylaminopurine (BAP) ranging from 0.00 to 3.5 mg/L in MS medium, where 2.5 mg/L BAP was proven to be the best level for regeneration. In a multiplication and root formation medium, 0.5 mg/L naphthalene acetic acid supported the maximum number of roots per plant. Finally, a direct somatic embryogenesis protocol was established, competent enough for simultaneous root/shoot induction. The results indicated that the plantlets were established within 12 weeks only. This in vitro regeneration protocol was fast and cost-effective and may be used for large-scale in vitro regeneration of sugarcane cultivars to save time and resources. The sugarcane cultivar SPF-234 remained the most responsive, followed by HSF-242 and CPF-246.

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Section: Introductionmentioning

confidence: 99%

Improving in vitro leaf disk regeneration system of sugarcane (Saccharum officinarum L.) with concurrent shoot/root induction from somatic embryos

Nawaz¹,

Ullah²,

Iqbal³

et al. 2013

Turk J Biol

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“…In particular, genetic engineering may not only be able to shorten the period of breeding time and reduce costs for producing an improved sugarcane line, it can also introduce new agronomic traits that are absent in the natural sugarcane germplasm (Jain et al 2007). For example, transgenic sugarcane plants were shown to produce sorbitol, gentiobiose and gentiobiitol (Chong et al 2007(Chong et al , 2010, and to confer resistance to herbicides or pest (Enríquez et al 2000;Weng et al 2006). Genetic transformation using insecticidal Bt genes is regarded as an effective method to develop insect-resistant transgenic plants (Kim et al 2008;Valderrama et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Transgenic sugarcane plants expressing high levels of modified cry1Ac provide effective control against stem borers in field trials

Weng

Hai-hua

et al. 2010

Transgenic Res

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To improve transgene expression level, we synthesized a truncated insecticidal gene m-cry1Ac by increasing its GC content from 37.4 to 54.8%, based on the codon usage pattern of sugarcane genes, and transferred it into two sugarcane cultivars (ROC16 and YT79-177) by microprojectile bombardment. The integration sites and expression pattern of the transgene were determined, respectively, by Southern, northern and western blot analyses. The transgenic sugarcane lines produced up to 50 ng Cry1Ac protein per mg soluble proteins, which was about fivefold higher than that produced by the partially modified s-cry1Ac (GC% = 47.5%). In greenhouse plant assay, about 62% of the transgenic lines exhibited excellent resistance to heavy infestation by stem borers. In field trials, the m-cry1Ac transgenic sugarcane lines expressing high levels of Cry1Ac were immune from insect attack. In contrast, expression of s-cry1Ac in transgenic sugarcane plants resulted in moderately decreased damages in internodes (0.4-1.7%) and stalks (13.3-26.7%) in comparison with the untransformed sugarcane controls, which showed about 4 and 26-40% damaged internodes and stalks, respectively. Significantly, these transgenic sugarcane lines with high levels of insect resistance showed similar agronomic and industrial traits as untransformed control plants. Taken together, the findings from this study indicate a promising potential of engineering an insect-resistant gene to tailor its protein expression levels in transgenic sugarcane to combat insect infestations.

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“…In one of the first studies to control fungal diseases through genetic engineering in sugarcane, a glucanase and a chitinase genes were expressed to confer resistance against Puccinia melanocephala , the causal agent of brown rust, but no results on resistance to infections by the fungus have been published ( Enriquez et al, 2000 ). In a similar approach, Nayyar et al (2017) reported the development of red rot resistant transgenic sugarcane through expression of a β-1,3-glucanase gene from Trichoderma spp.…”

Section: Resistance To Biotic Stressesmentioning

confidence: 99%

Genetic Transformation of Sugarcane, Current Status and Future Prospects

et al. 2021

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Sugarcane (Saccharum spp.) is a tropical and sub-tropical, vegetative-propagated crop that contributes to approximately 80% of the sugar and 40% of the world’s biofuel production. Modern sugarcane cultivars are highly polyploid and aneuploid hybrids with extremely large genomes (>10 Gigabases), that have originated from artificial crosses between the two species, Saccharum officinarum and S. spontaneum. The genetic complexity and low fertility of sugarcane under natural growing conditions make traditional breeding improvement extremely laborious, costly and time-consuming. This, together with its vegetative propagation, which allows for stable transfer and multiplication of transgenes, make sugarcane a good candidate for crop improvement through genetic engineering. Genetic transformation has the potential to improve economically important properties in sugarcane as well as diversify sugarcane beyond traditional applications, such as sucrose production. Traits such as herbicide, disease and insect resistance, improved tolerance to cold, salt and drought and accumulation of sugar and biomass have been some of the areas of interest as far as the application of transgenic sugarcane is concerned. Although there have been much interest in developing transgenic sugarcane there are only three officially approved varieties for commercialization, all of them expressing insect-resistance and recently released in Brazil. Since the early 1990’s, different genetic transformation systems have been successfully developed in sugarcane, including electroporation, Agrobacterium tumefaciens and biobalistics. However, genetic transformation of sugarcane is a very laborious process, which relies heavily on intensive and sophisticated tissue culture and plant generation procedures that must be optimized for each new genotype to be transformed. Therefore, it remains a great technical challenge to develop an efficient transformation protocol for any sugarcane variety that has not been previously transformed. Additionally, once a transgenic event is obtained, molecular studies required for a commercial release by regulatory authorities, which include transgene insertion site, number of transgenes and gene expression levels, are all hindered by the genomic complexity and the lack of a complete sequenced reference genome for this crop. The objective of this review is to summarize current techniques and state of the art in sugarcane transformation and provide information on existing and future sugarcane improvement by genetic engineering.

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Sugarcane (Saccharum hybrid) Genetic Transformation Mediated by Agrobacterium tumefaciens: Production of Transgenic Plants Expressing Proteins with Agronomic and Industrial Value

Cited by 16 publications

References 13 publications

Improving in vitro leaf disk regeneration system of sugarcane (Saccharum officinarum L.) with concurrent shoot/root induction from somatic embryos

Improving in vitro leaf disk regeneration system of sugarcane (Saccharum officinarum L.) with concurrent shoot/root induction from somatic embryos

Transgenic sugarcane plants expressing high levels of modified cry1Ac provide effective control against stem borers in field trials

Genetic Transformation of Sugarcane, Current Status and Future Prospects

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