Sugars and sucrose transporters in pollinia ofPhalaenopsis aphrodite(Orchidaceae)

Abstract

The pollen grains of Phalaenopsis orchids are clumped tightly together, packed in pollen dispersal units called pollinia. In this study, the morphology, cytology, biochemistry, and sucrose transporters in pollinia of Phalaenopsis orchids were investigated. Periodic acid–Schiff (PAS) counterstained with DAPI or aniline blue was used to characterize the distribution of sugars and callose at the different development stages of pollinia. Ultra performance liquid chromatography-high resolution-tandem mass spectrometry data indicated that Phal. orchid accumulated abundant saccharides such as sucrose, galactinol, myo-inositol, and glucose, and trace amounts of raffinose and trehalose in mature pollinia. We found that galactinol synthase (PAXXG304680) and trehalose-6-phosphate phosphatase (PAXXG016120) were preferentially expressed in mature pollinia. The Phal. aphrodite genome was identified as having 11 sucrose transporters (SUTs). Our qRT-PCR confirmed that two SUTs (PAXXG030250 and PAXXG195390) were preferentially expressed in the pollinia. Pollinia germinated in Brewbaker and Kwack’s medium (BK) pollen germination media supplemented with 10% sucrose showed increased callose production and enhanced pollinia germination, but there was no callose and no germination in BK media without sucrose. We postulate that Phal. orchid accumulates high levels of sugars in mature pollinia providing nutrients and enhanced sucrose transporter gene expression for pollinia germination and tube growth.

Anatomy and Comparative Transcriptome Reveal the Mechanism of Male Sterility in Salvia miltiorrhiza

“…Conversely, sterile pollen grains do not have any substantial filling and they are shriveled ( Figure 2 E and Figure 4 M–O). As a precursor of starch synthesis, trehalose is important for regulating plant development and the downregulation of trehalose-phosphate phosphatase (TPP) proteins in anthers cause male sterility [ 40 , 41 ]. TPP (PAXXG016120) is expressed in mature pollen of Phalaenopsis aphrodite to accumulate high levels of sugars, and it provides nutrients and sucrose for pollen germination [ 41 ].…”

“…As a precursor of starch synthesis, trehalose is important for regulating plant development and the downregulation of trehalose-phosphate phosphatase (TPP) proteins in anthers cause male sterility [ 40 , 41 ]. TPP (PAXXG016120) is expressed in mature pollen of Phalaenopsis aphrodite to accumulate high levels of sugars, and it provides nutrients and sucrose for pollen germination [ 41 ]. Similarly, we find that DEGs encoding TPP protein have higher expression levels in SD than SC at the same stage, such as SmiChr073137 ( Table S3 ).…”