Suggested Guidelines for Immunohistochemical Techniques in Veterinary Diagnostic Laboratories

Ja, Ramos-Vara; Kiupel, Matti; Baszler, Timothy V.; Bliven, L; Brodersen, Bruce W.; Chelack, Brian J.; Czub, Stefanie; F, Del Piero; Dial, Sharon M.; Ej, Ehrhart; Graham, Tanya; Manning, Lisa; Paulsen, Daniel B.; Ve, Valli; West, Keith

doi:10.1177/104063870802000401

Cited by 301 publications

(304 citation statements)

References 75 publications

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“…Accurate diagnosis is essential to determine a prognosis and treatment (Fernandez et al 2005). Immunohistochemistry (IHC) has been proven to be one of the most important ancillary techniques in the characterization of neoplastic diseases in veterinary medicine, as oncologists demand more speciϐic diagnoses (Ramos-Vara et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Histomorphological and immunohistochemical characterization of 172 cutaneous round cell tumours in dogs

Araújo

Preis

Lavalle³

et al. 2012

Pesq. Vet. Bras.

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Pesq. Vet. , cytokeratin, vimentin and E-cadherin) on formalin-ϐixed, parafϐin-embedded sections of canine cutaneous round cell tumours. Neoplastic tumours were diagnosed by histology and histochemical stains and included 107 mast cell tumours, 31 cutaneous histiocytomas, two localized histiocytic sarcomas, 21 cutaneous lymphomas, three plasma cell tumours, one transmissible venereal tumour and seven unclassiϐied round cell tumours. The histologic diagnosis was modiϐied in 39.5% of the total 172 neoplasms. The staining for CD45 and Ecadherin were variable, and therefore, the ϐinal diagnoses of cutaneous histiocytoma and localized histiocytic sarcoma were made based on histology in association with negative results for CD3, CD79a, CD117 and cytokeratin. The cellular origin of unclassiϐied round cell tumours was deϐined in all cases. Cutaneous B-cell lymphoma and plasma cell tumours were CD79a-positive and could be distinguished from each other by the morphological characteristics. Mast cell tumours and T cell lymphoma were CD117 and CD3 positive, respectively. The positive staining for vimentin and the negative staining for CD3, CD79a, CD117 and cytokeratin favoured the diagnosis of transmissible venereal tumours. Thus, the ϐinal diagnosis of cutaneous round cell tumours should be based on the interpretation of immunohistochemical results together with the cellular morphology observed by histology. Therefore, more studies to optimize the speciϐic markers in formalin-ϐixed, parafϐin-embedded tissues (especially for histiocytes) are required for deϐinitive diagnosis of round cell tumours in dogs. las redondas em cães. Os tumores foram diagnosticados usando-se a histopatologia e a marcação imuno-histoquí-mica. Foram incluídos 107 mastocitomas, 31 histiocitomas cutâneos, 2 sarcomas histiocíticos localizados, 21 linfomas cutâneos, 3 plasmocitomas, 1 tumor venéreo transmissí-vel e 7 tumores de células redondas não classiϐicados. O diagnóstico histológico foi modiϐicado em 39,5% do total de 172 neoplasias. A marcação do anticorpo CD45 e E--caderina foi variável e, nesse sentido, o diagnóstico ϐinal de histiocitoma cutâneo e sarcoma histiocítico localizado foi baseado na histologia em associação com os resultados negativos para CD3, CD79a, CD117 e citoqueratina. A origem celular dos tumores de células redondas não classiϐicados foi deϐinida em todos os casos. foram distinguidos entre si pelas características morfológi-cas. Marcação positiva para vimentina e negativa para CD3, CD79a, CD117 e citoqueratina favoreceram o diagnóstico dos tumores venereos transmissíveis. Assim, o diagnósti-co ϐinal dos tumores de células redondas foram baseados na interpretação dos resultados da imuno-histoquímica em conjunto com a avaliação das características morfoló-gicas observadas na histologia. Finalmente, mais estudos em relação à padronização de marcadores especíϐicos para tecidos paraϐinizados (especialmente para histiócitos) são necessários para o diagnóstico deϐinitivo das neoplasias de células redondas em cães.TERMOS DE INDEXAÇÃO...

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Section: Introductionmentioning

confidence: 99%

Histomorphological and immunohistochemical characterization of 172 cutaneous round cell tumours in dogs

Araújo

Preis

Lavalle³

et al. 2012

Pesq. Vet. Bras.

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“…However, some authors described similar immunostaining in histiocytomas. In this case, differential diagnosis is based on clinical and histopathological criteria (Mozos et al 1996, Ramos-Vara et al 2008.…”

Section: Discussionmentioning

confidence: 99%

Immunocytochemical characterization of primary cell culture in canine transmissible venereal tumor

et al. 2016

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RESUMO.-[Caracterização imunocitoquímica de culturas primárias de tumor venéreo transmissível canino.]Os anticorpos anti-vimentina, anti-lisozima, anti-alfa 1 antitripsina, anti-CD3 e anti-CD79α foram empregados para a caracterização de culturas primárias de tumor venéreo transmissível canino (TVT). Amostras para cultura primá-ria e imuno-histoquímica foram coletadas de oito cães com diagnóstico clínico e citológico de TVT. Para validar o resultado inmunocitoquímico nas culturas de TVT foi realizada a contagem de cromossomos. Para a análise estatística o teste de Mann-Whitney foi empregado a um nível de significância de p<0.05. As culturas e os tecidos de TVT apresentaram intensa reatividade para vimentina, moderada a leve para Lisozima, moderada para alfa-antitripsina e não houve marcação para CD3 e CD79α. Immunochemistry with anti-vimentin, anti-lysozyme, anti-alpha 1 antitrypsin, anti-CD3 and anti-CD79α antibodies has been used for characterization of primary cell culture in the transmissible venereal tumor (TVT). Samples for primary cell culture and immunohistochemistry assays were taken from eight dogs with cytological and clinical diagnosis of TVT. To validate the immunochemical results in the primary cell culture of TVT, a chromosome count was performed. For the statistical analysis, the Mann-Whitney test with p<0.05 was used. TVT tissues and culture cells showed intense anti-vimentin immunoreactivity, lightly to moderate immunoreactivity for anti-lysozyme, and mild for anti-alpha-antitrypsin. No marking was achieved for CD3 and CD79α. All culture cells showed chromosomes variable number of 56 to 68. This is the first report on the use of immunocytochemical characterization in cell culture of TVT. Significant statistic difference between immunochemistry in tissue and culture cell was not established, what suggests that the use of this technique may provide greater certainty for the confirmation of tumors in the primary culture. This fact is particularly important because in vitro culture of tumor tissues has been increasingly used to provide quick access to drug efficacy and presents relevant information to identify potential response to anticancer medicine; so it is possible to understand the behavior of the tumor. Immunocytochemical characterization of primary cell culture in transmissible venereal tumor o uso da immunocitoquímica para a caracterização de culturas de TVT. Assim, e devido ao fato de se observar semelhança entre a imunomarcação em células e tecidos, sugere-se que o uso desta técnica possa auxiliar na confirmação de culturas primárias do tumor, fato muito importante porque a utilização da cultura do tumor pode permitir o acesso a informação relevante sobre resposta potencial a um tratamento e conhecimento do comportamento biológico do tumor.TERMOS DE INDEXAÇÃO: Tumor venéreo transmissível canino, alfa 1 antitripsina, citogenética, lisozima, vimentina.

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“…Sections were counterstained with Mayer's haematoxylin. The negative control sections were prepared by excluding the primary antibodies [34] . Positive controls for p53 were MDA and JK cell extracts, HeLa cells and Human breast carcinoma tissue.…”

Section: Methodsmentioning

confidence: 99%

The Potential Protective Role of Hesperidin Against Capecitabine-Induced Corneal Toxicity in Adult Male Albino Rat. Light and Electron Microscopic Study

Elwan

Kassab

2017

Egyptian Journal of Histology

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Background: Capecitabine is a chemotherapeutic agent widely used for the treatment of malignancies. Ocular disorders have been reported with capecitabine therapy. Hesperidin is a naturally occurring compound derived mainly from citrus fruits and has a wide range of pharmacological activities and a proposed role in combating many ocular diseases. Aim: To evaluate the potential protective role of hesperidin against the corneal toxicity caused by capecitabine in adult male albino rats. Material and Methods: Thirty-six adult male albino rats were divided into four equal groups; control group, hesperidintreated group (50 mg/kg), capecitabine-treated group (40 mg/kg), and combination-treated "capecitabine and hesperidintreated" group. Animals were orally administered once daily for one month. Specimens from the cornea were processed for light and electron microscopy. Immunohistochemical study was performed using antibodies against p53. Results: Specimens from capecitabine-treated animals showed significant decrease of epithelial thickness. The corneal epithelial cells showed nuclear alteration and vacuolated cytoplasm. The stromal collagen fibers were irregularlyarranged and widely separated with neovascularization and mononuclear cellular infiltration. Ultrastructurally, focal widening of the intercellular spaces, partial loss of desmosomal junctions and swollen mitochondria were observed. The immunohistochemical study showed a significant increase in p53 immunoreaction. In contrast, minimal changes were observed in rats treated concomitantly with both capecitabine and hesperidin, with a non significant increase in the immunoreactions. Conclusion: Capecitabine induced structural changes in cornea of adult albino rat that could be ameliorated by concomitant treatment with hesperidin.

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Suggested Guidelines for Immunohistochemical Techniques in Veterinary Diagnostic Laboratories

Cited by 301 publications

References 75 publications

Histomorphological and immunohistochemical characterization of 172 cutaneous round cell tumours in dogs

Histomorphological and immunohistochemical characterization of 172 cutaneous round cell tumours in dogs

Immunocytochemical characterization of primary cell culture in canine transmissible venereal tumor

The Potential Protective Role of Hesperidin Against Capecitabine-Induced Corneal Toxicity in Adult Male Albino Rat. Light and Electron Microscopic Study

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