Suitability of R. pulmo Jellyfish-Collagen-Coated Well Plates for Cytocompatibility Analyses of Biomaterials

Abstract

Cytocompatibility analyses of new implant materials or biomaterials are not only prescribed by the Medical Device Regulation (MDR), as defined in the DIN ISO Norm 10993-5 and -12, but are also increasingly replacing animal testing. In this context, jellyfish collagen has already been established as an alternative to mammalian collagen in different cell culture conditions, but a lack of knowledge exists about its applicability for cytocompatibility analyses of biomaterials. Thus, the present study was conducted to compare well plates coated with collagen type 0 derived from Rhizostoma pulmo with plates coated with bovine and porcine collagen. The coated well plates were analysed in vitro for their cytocompatibility, according to EN ISO 10993-5/−12, using both L929 fibroblasts and MC3T3 pre-osteoblasts. Thereby, the coated well plates were compared, using established materials as positive controls and a cytotoxic material, RM-A, as a negative control. L929 cells exhibited a significantly higher viability (#### p < 0.0001), proliferation (## p < 0.01), and a lower cytotoxicity (## p < 0.01 and # p < 0.05)) in the Jellagen® group compared to the bovine and porcine collagen groups. MC3T3 cells showed similar viability and acceptable proliferation and cytotoxicity in all collagen groups. The results of the present study revealed that the coating of well plates with collagen Type 0 derived from R. pulmo leads to comparable results to the case of well plates coated with mammalian collagens. Therefore, it is fully suitable for the in vitro analyses of the cytocompatibility of biomaterials or medical devices.

The Structure Features and Improvement of Concrete Properties with Dead Jellyfish Mass

“…The study showed the positive effect of dead jellyfish mass on the rheological characteristics of the mixture, namely on mobility, as well as on the mechanical characteristics of hardened concrete. This study, as well as previously known research considering the use of dead jellyfish mass in other sectors of the economy [16][17][18][19], provided impetus for additional research to study the effect of using Black Sea dead jellyfish as a plasticizing additive in cement composites and the development of existing ideas about the structure formation and properties of concrete using dead jellyfish mass.…”

Biphasic bone substitutes coated with PLGA incorporating therapeutic ions Sr2+ and Mg2+: cytotoxicity cascade and in vivo response of immune and bone regeneration

“…To evaluate the cytocompatibility of the BBS combined with 18 coatings based on the bioactive cations strontium (Sr 2+ ) and magnesium (Mg 2+ ) as well as PLGA coating, XTT cell viability assays were conducted using L929-fibroblasts and Normal Human Osteoblasts (NHOsts) following the DIN ISO 10993-5 protocol as previously described ( Gogele et al, 2020 ; Schroepfer et al, 2020 ; Pantermehl et al, 2022 ; Ren et al, 2023 ). Based on the cytocompatibility analyses, the material types with a viability above 70% compared to the negative control group as defined within the DIN ISO norm were considered cytocompatible and used for further material characterizations and in vivo studies.…”