Suitability of tissue tolerable plasmas (TTP) for the management of chronic wounds

Krämer, Axel; Lademann, Juergen; Bender, Claudia; Sckell, Axel; Hartmann, Bernd; Münch, Sandra; Hinz, Peter; Ekkernkamp, Axel; Matthes, Rutger; Koban, Ina; Partecke, I.; Heidecke, Claus–Dieter; Masur, Kai; Reuter, Stephan; Weltmann, Klaus‐Dieter; Koch, Stefan; Assadian, Ojan

doi:10.1016/j.cpme.2013.03.002

Cited by 94 publications

(72 citation statements)

References 54 publications

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“…Plasma treatment has recently been shown to support human and animal wound healing [21,22] but little is known about its intracellular effects on primary human monocytes, which are the key players in the process of wound recovery. Thus, we investigated the modulation of intracellular signaling effects of primary monocytes by the non-thermal atmospheric pressure plasma jet kINPen 09, which was previously done for the THP-1 monocyte cell line [23].…”

Section: Introductionmentioning

confidence: 99%

Non-thermal plasma treatment induces MAPK signaling in human monocytes

et al. 2014

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Abstract:The application of non-thermal atmospheric pressure plasma raises a hope for the new wound healing strategies. Next to its antibacterial effect it is known to stimulate skin cells. However, monocytes are also needed for the complex process of a wound healing. This study investigates the impact of plasma on the intracellular signaling events in the primary human monocytes. The proliferative MEK-ERK (MAPK/ERK kinase-extracellular signal-regulated kinase) pathway was activated by short plasma treatment times. In contrast, an induction of the apoptotic JNK (c-Jun N-terminal kinase) cascade as well as activation of caspase 3 were observed after long plasma exposure. These findings indicate that monocytes can be differentially stimulated by plasma treatment and may contribute to the proper wound recovery.

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Section: Introductionmentioning

confidence: 99%

Non-thermal plasma treatment induces MAPK signaling in human monocytes

et al. 2014

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“…[1][2][3][4][5] The strongly non-equilibrium chemistry along with their physical properties of energy transfer into sensible surfaces makes them excellently suited for treatment of, e.g., chronic wounds. [6][7][8][9] The energy dissipated within the plasma is transferred into thermal, chemical, electronic, and radiative energy, to name the most relevant examples. The gas and plasma phase chemistry of these jets has been investigated quite thoroughly, and many processes have been already understood.…”

Section: Introductionmentioning

confidence: 99%

Impact of plasma jet vacuum ultraviolet radiation on reactive oxygen species generation in bio-relevant liquids

et al. 2015

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Plasma medicine utilizes the combined interaction of plasma produced reactive components. These are reactive atoms, molecules, ions, metastable species, and radiation. Here, ultraviolet (UV, 100–400 nm) and, in particular, vacuum ultraviolet (VUV, 10–200 nm) radiation generated by an atmospheric pressure argon plasma jet were investigated regarding plasma emission, absorption in a humidified atmosphere and in solutions relevant for plasma medicine. The energy absorption was obtained for simple solutions like distilled water (dH2O) or ultrapure water and sodium chloride (NaCl) solution as well as for more complex ones, for example, Rosewell Park Memorial Institute (RPMI 1640) cell culture media. As moderate stable reactive oxygen species, hydrogen peroxide (H2O2) was studied. Highly reactive oxygen radicals, namely, superoxide anion (O2•−) and hydroxyl radicals (•OH), were investigated by the use of electron paramagnetic resonance spectroscopy. All species amounts were detected for three different treatment cases: Plasma jet generated VUV and UV radiation, plasma jet generated UV radiation without VUV part, and complete plasma jet including all reactive components additionally to VUV and UV radiation. It was found that a considerable amount of radicals are generated by the plasma generated photoemission. From the experiments, estimation on the low hazard potential of plasma generated VUV radiation is discussed.

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“…Von Woedtke et al 18 classified plasma for medical application according to two general principles: indirect use of plasma to treat surfaces, materials, or devices for medical applications, and application of physical plasma on or within the human body. Sterilization of medical materials or devices (e.g., medical implants, catheters, or materials in blood purification systems) is the main use of indirect plasmas for medical purposes; 20 whereas physical plasmas are under intense study for applications in wound healing, 19,20 blood coagulation and skin regeneration, 21 dentistry, 22 and apoptosis of cancer cells. 23 To date, research on ACP sterilization has demonstrated effective reduction of clinically important microorganisms in their planktonic state or in biofilms.…”

Section: Introductionmentioning

confidence: 99%

Dielectric Barrier Discharge Atmospheric Cold Plasma for Inactivation of Pseudomonas aeruginosa Biofilms

Ziuzina¹,

Patil²,

Cullen

et al. 2014

Plasma Med

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ABSTRACT:In recent years, atmospheric cold plasma (ACP) has been widely investigated for potential application as an alternative decontamination technology in biomedical and healthcare sectors. In this study, the antimicrobial efficacy of ACP against Pseudomonas aeruginosa biofilms was investigated. The 48-h biofilms were treated inside sealed polypropylene containers with a high-voltage dielectric barrier discharge (DBD) ACP (80 kV RMS ) and subsequently stored for 24 h at room temperature. Treatment for 60 s by either the direct or indirect mode of ACP exposure (inside or outside plasma discharge, respectively) reduced bacterial populations by an average of 5.4 log cycles from an initial 6.6 log 10 CFU/mL. Increasing the treatment time from 60 s to 120 s and 300 s reduced biofilms to undetectable levels. According to XTT assay (a metabolic activity assay), an extended treatment time of 300 s was necessary to reduce metabolic activity of cells in biofilms by an average of 70%. Further investigation of biofilm viability by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) demonstrated that extended ACP treatment had a detrimental effect on the viability of P. aeruginosa through disintegration of both bacterial cells and the biofilm matrix. The results of this study demonstrate the potential of a novel, in-package, high-voltage ACP decontamination approach for the inactivation of bacterial biofilms.

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Suitability of tissue tolerable plasmas (TTP) for the management of chronic wounds

Cited by 94 publications

References 54 publications

Non-thermal plasma treatment induces MAPK signaling in human monocytes

Non-thermal plasma treatment induces MAPK signaling in human monocytes

Impact of plasma jet vacuum ultraviolet radiation on reactive oxygen species generation in bio-relevant liquids

Dielectric Barrier Discharge Atmospheric Cold Plasma for Inactivation of Pseudomonas aeruginosa Biofilms

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