Suitability of ultra-high performance liquid chromatography for the determination of fat-soluble nutritional status (vitamins A, E, D, and individual carotenoids)

Granado-Lorencio, Fernando; Herrero-Barbudo, Carmen; Blanco–Navarro, I.; Pérez-Sacristán, Belen

doi:10.1007/s00216-010-3655-2

Cited by 66 publications

(53 citation statements)

References 11 publications

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“…The development of columns of very small particle size allows for the use of much lower flows of mobile phase at very high pressures. UHPLC has been used for the determination of fat-soluble vitamins but less so for hydrosoluble ones (apart from some mentions of manufacturers brochures) [18,19]. Xingnan and Adrian [20] developed and validated an UHPLC method for the quantification of serum L-AA and DHAA, by the subtraction method.…”

Section: Introductionmentioning

confidence: 99%

An improved and fast UHPLC-PDA methodology for determination of L-ascorbic and dehydroascorbic acids in fruits and vegetables. Evaluation of degradation rate during storage

et al. 2012

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This study provides a versatile validated method to determine the total vitamin C content, as the sum of the contents of L-ascorbic acid (L-AA) and dehydroascorbic acid (DHAA), in several fruits and vegetables and its degradability with storage time. Seven horticultural crops from two different origins were analyzed using an ultrahigh-performance liquid chromatographic-photodiode array (UHPLC-PDA) system, equipped with a new trifunctional high strength silica (100% silica particle) analytical column (100 mm×2.1 mm, 1.7 μm particle size) using 0.1% (v/v) formic acid as mobile phase, in isocratic mode. This new stationary phase, specially designed for polar compounds, overcomes the problems normally encountered in HPLC and is suitable for the analysis of large batches of samples without L-AA degradation. In addition, it proves to be an excellent alternative to conventional C18 columns for the determination of L-AA in fruits and vegetables. The method was fully validated in terms of linearity, detection (LOD) and quantification (LOQ) limits, accuracy, and inter/intraday precision. Validation experiments revealed very good recovery rate of 96.6±4.4% for L-AA and 103.1±4.8 % for total vitamin C, good linearity with r 2 -values >0.999 within the established concentration range, excellent repeatability (0.5%), and reproducibility (1.6%) values. The LOD of the method was 22 ng/mL whereas the LOQ was 67 ng/mL. It was possible to demonstrate that L-AA and DHAA concentrations in the different horticulture products varied oppositely with time of storage not always affecting the total amount of vitamin C during shelf-life. Locally produced fruits have higher concentrations of vitamin C, compared with imported ones, but vegetables showed the opposite trend. Moreover, this UHPLC-PDA methodology proves to be an improved, simple, and fast approach for determining the total content of vitamin C in various food commodities, with high sensitivity, selectivity, and resolving power within 3 min of run analysis.

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Section: Introductionmentioning

confidence: 99%

An improved and fast UHPLC-PDA methodology for determination of L-ascorbic and dehydroascorbic acids in fruits and vegetables. Evaluation of degradation rate during storage

et al. 2012

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“…Ultra high-performance liquid chromatography (UHPLC) has been used to monitor carotenoids, but mostly in conjunction with other fat-soluble vitamins [224,225]. UHPLC methods for the quantification of a wide range of carotenoids are uncommon.…”

Section: Chromatographic Methodsmentioning

confidence: 99%

New Developments in Lycopene Analysis by Spectroscopic and Chromatographic Techniques, Accompanied With Mathematical Modelling

Cáceres¹,

Cámara²,

Manzoor³

et al. 2015

Acta Hortic.

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“…The intervention study consisted of the supplementation of the diet with 1 9 250 mL milk-based fruit drink/day for 4 weeks with a washout period of 4 weeks in between. Overnight fasting blood samples were collected before and after each supplementation period (day 0 and 28) for the analysis of b-Cx in serum (exposition marker) by a quality-controlled ultra-fast liquid chromatography method (Granado-Lorencio et al 2010) and to assess the effect of bCx and PS by monitoring changes in the lipid profile [TC, c-LDL and HDL-cholesterol (c-HDL)]. …”

Section: Intervention Studymentioning

confidence: 99%

β-Cryptoxanthin modulates the response to phytosterols in post-menopausal women carrying NPC1L1 L272L and ABCG8 A632 V polymorphisms: an exploratory study

et al. 2014

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Phytosterol (PS) intake may be used for hypercholesterolaemia in some groups although the presence of non-responders is well known. Carotenoids and PS/ cholesterol may compete for the same transporters during absorption. As part of a randomized, double-blind, crossover, multiple-dose supplementation study with b-cryptoxanthin (b-Cx) and PS, single and combined, polymorphisms of ABCG8 (A632V) and NCPL1 (L272L) were determined in 19 post-menopausal women. Subjects carrying CC polymorphism for NCP1L1 (L272L) showed a net increase in total cholesterol and LDL after PS intake but, interestingly, displayed a decrease in both lipid fractions after consuming PS plus b-Cx. For the ABCG8 (A632V) gene, CT/TT carriers consuming PS also displayed an increase in total cholesterol and LDL, but this increment was much lower after the intake of PS plus b-Cx. Additionally, in CC carriers for ABCG8 (A632V), a greater decrease in total cholesterol and LDL was found after the intake of PS plus b-Cx compared to that observed after PS alone. Overall, our results suggest that b-Cx improves the response to PS in individuals carrying specific genetic polymorphisms (i.e. non-responders), opening the possibility to modulate the response to PS by food technology. (ClinicalTrials.gov NCT01074723).

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Suitability of ultra-high performance liquid chromatography for the determination of fat-soluble nutritional status (vitamins A, E, D, and individual carotenoids)

Cited by 66 publications

References 11 publications

An improved and fast UHPLC-PDA methodology for determination of L-ascorbic and dehydroascorbic acids in fruits and vegetables. Evaluation of degradation rate during storage

An improved and fast UHPLC-PDA methodology for determination of L-ascorbic and dehydroascorbic acids in fruits and vegetables. Evaluation of degradation rate during storage

New Developments in Lycopene Analysis by Spectroscopic and Chromatographic Techniques, Accompanied With Mathematical Modelling

β-Cryptoxanthin modulates the response to phytosterols in post-menopausal women carrying NPC1L1 L272L and ABCG8 A632 V polymorphisms: an exploratory study

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