Sulfated hyaluronan‐containing artificial extracellular matrices promote proliferation of keratinocytes and melanotic phenotype of melanocytes from the outer root sheath of hair follicles

Abstract

The aim of this work was to establish improved cultivation conditions for human keratinocytes (HUKORS) and melanocytes (HUMORS) from the outer root sheath (ORS) of human hair follicles for purposes of generating an epidermal graft. To this end, the cells were cultivated on artificial extracellular matrix coatings composed of collagen (COLL) and hyaluronan (HA) with varying sulfation degrees. HUKORS and HUMORS were characterized based on their morphology and proliferation, marker gene expression, protein expression and melanin content in melanocytes. Depending on the sulfation degree, the matrices provided a favorable proliferation environment for HUKORS and improved the balance between proliferation and the exertion of melanotic phenotype (gene expression and melanin content) in HUMORS. Based on the increased gene expression of microphthalmia‐associated transcription factor, as well as the downstream‐affected melanotic genes premelanosome protein and tyrosinase in HUMORS cultivated on collagen matrices with high‐sulfated HA, we assume that sulfated HA enhanced melanotic phenotype either by directly binding the CD44 receptor or by concentrating signaling mediators on site. Being a promising cultivation environment for both HUKORS and HUMORS, collagen matrices with sulfated HA have a potential of significantly improving the development of ORS‐based epidermal grafts. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1640–1653, 2019.

Self-Assembled Sulfated Hyaluronan Coating Modulates Transforming Growth Factor-Beta1 Penetration for Corneal Scarring Alleviation

“…In previous research, sHA showed great biocompatibility and had been used as a main component to synthesize biomaterials. − However, Dieter et al reported that sHA reduces the proliferation rate of primary rat calvarial osteoblasts by affecting their cell cycle . Similar results were found in our study.…”

“…After sHA is immobilized on the collagen surface, its interaction with free cytokines in the medium will be reduced. , Therefore, the proliferation of corneal epithelial cells on collagen membranes with sHA coatings is unaffected. Studies on biomaterials using sHA as main raw materials have shown that immobilized sHA had no adverse effect on cell proliferation. ,, The results of our animal experiments also confirmed that the re-epithelialization of the wounded cornea was unaffected by the sHA coating, demonstrating that the application of sHA by the coating method is biocompatible.…”

“…Studies on biomaterials using sHA as main raw materials have shown that immobilized sHA had no adverse effect on cell proliferation. 44,46,51 The results of our animal experiments also confirmed that the re-epithelialization of the wounded cornea was unaffected by the sHA coating, demonstrating that the application of sHA by the coating method is biocompatible.…”

Laccase-Treated Polystyrene Surfaces with Caffeic Acid, Dopamine, and L-3,4-Dihydroxyphenylalanine Substrates Facilitate the Proliferation of Melanocytes and Embryonal Carcinoma Cells NTERA-2

“…carcinoma cell lines [37,38]. We assumed that the weak adherence of both cell types could be reinforced by activating the PS surface chemistry, which could subsequently corroborate cell proliferation.…”

“…To the best of our knowledge, this study is the first assessment of the laccase-based surface modification effects on those two cell lines [ 33 , 34 , 35 , 36 ]. NHEMs are well known for easily detaching in the process of differential trypsinization, and the adherence of NTERA-2 cells is low as in most embryonic carcinoma cell lines [ 37 , 38 ]. We assumed that the weak adherence of both cell types could be reinforced by activating the PS surface chemistry, which could subsequently corroborate cell proliferation.…”