Sulfhydryl reactive microspheres for the efficient delivery of thiolated bioactive cargoes

A novel gene carrier prepared from triple helical β-glucan and polydeoxyadenylic acid

“…38 Therefore, such gene-delivery systems containing disulde linkages are attractive because tumor tissues are highly hypoxic compared with normal tissues, 39 with at least 4-fold higher GSH concentration. 40 With the aid of this biotechnology, novel carriers containing disulde bonds have been widely applied in cargo delivery such as plasmid DNA, 1 small interference RNA (siRNA), [41][42][43] oligonucleotides, proteins 44,45 and hydrophobic drugs. 46 Based on the above, here, we propose an alternative strategy by incorporating disulde bonds between the binding site of poly(dA) and the target DNA sequence (poly(dA)-SS-DNA) to allow the automatic cleavage of disulde bonds in the cytoplasm.…”

Tracking Cell Proliferation Using a Nanotechnology-Based Approach

“…These nanodevices have been extensively used as systems for in vitro applications [7][8][9]. Furthermore, these NPs enable solid phase multistep chemistries to become compatible with different bio-orthogonal strategies [10,11]. Easy entry in a broad range of cell types including adherent, suspensions and primary cell lines has been reported [8][9][10][11][12].…”

Cellular response to empty and palladium‐conjugated amino‐polystyrene nanospheres uptake: A proteomic study

“…Currently, more than 150 different companies are developing nanospheres‐based therapeutic products . In previous studies Sanchez‐Martin et al synthesized monodisperse amino‐functionalized polystyrene nanospheres and their cellular uptake successfully demonstrated in a broad range of cell types . Relevant cargoes such as proteins, oligonucleotides, both DNA plasmids and siRNA, ion sensors, fluorophores, and metals such as palladium have been conjugated to them and successfully delivered inside cells.…”