Sulfitobacter faviae sp. nov., isolated from the coral Favia veroni

Abstract

Three closely related, non-sporulating, aerobic, Gram-stain-negative, motile, rod-shaped isolates (S5-53 T , S6-62 and S6-64) were obtained from mucus of corals Favia veroni from the Andaman Sea, India. Colonies grown on marine agar were small, circular and cream-coloured. Heterotrophic growth was observed at 10-40 C and pH 6-10; optimum growth occurred at The genus Sulfitobacter belongs to the family Rhodobacteraceae in the class Alphaproteobacteria. The genus was introduced by Sorokin (1995) to accommodate two strains of heterotrophic bacteria isolated from the Black Sea with Sulfitobacter pontiacus as the type species. At the time of writing, the genus Sulfitobacter contains 15 recognized species (Parte, 2014) that were isolated from marine environments either from seawater (Sorokin, 1995;Kwak et al., 2014) or associated with marine invertebrates (Fukui et al., 2014;Hong et al., 2015) from different geographical regions and thought to play an important role in organic sulfur cycling in the ocean environment (Ivanova et al., 2004). Phenotypically, strains are Gram-negative, aerobic and rod-shaped. All strains of the genus grow optimally at 25-30 C and pH 7.0-8.0 and require NaCl for growth. Q10 is the predominant quinone. All strains are catalase-and oxidase-positive. Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and phosphatidylethanolamine are the main polar lipids and C 18 : 1 !7c, C 16 : 0 and C 10 : 0 3-OH are the major fatty acids. The DNA G+C content varies between 55 and 63.7 mol%. Based on 16S rRNA gene sequence analysis, members of this genus are closely related to the monotypic genus Oceanibulbus (Wagner-Döbler et al., 2004) T in the phylogenetic tree (Ivanova et al., 2004;Kwak et al., 2014;Fukui et al., 2014).In the present study, we describe the phenotypic, genotypic and chemotaxonomic characteristics of three strains, i.e. S5-53 T , S6-62 and S6-64, isolated from mucus of the coral Favia veroni from the Andaman Sea, India. Based on 16S rRNA gene sequences, strain S5-53 T is shown to have a close phylogenetic relationship with the members of the genera Sulfitobacter and Oceanibulbus. A detailed taxonomic study demonstrated that the strains constituted a novel species of the genus Sulfitobacter and strain S5-53 T has been proposed as the type strain.Multiple specimens of the coral Favia veroni were collected from sea near Henry Lawrence island, Andaman Sea (12

Antarcticimicrobium sediminis gen. nov., sp. nov., isolated from Antarctic intertidal sediment, transfer of Ruegeria lutea to Antarcticimicrobium gen. nov. as Antarcticimicrobium luteum comb. nov.

“…n. microbium a Table 2. Differential characteristics of the genus Antarcticimicrobium and closely related genera in the family Rhodobacteraceae Genera: 1, Antarcticimicrobium gen. nov. (strain S4J41 T and Ruegeria lutea JCM 30927 T , this study); 2, Ruegeria [30,[37][38][39][40][41][42][43][44][45][46][47][48][49][50][51]; 3, Phaeobacter (this study and [30,[52][53][54][55][56]); 4, Puniceibacterium (this study and [5,57]); 5, Sulfitobacter (this study and [58][59][60][61][62][63][64][65][66][67][68][69][70][71][72][73]); 6, Pseudopuniceibacterium [36]. +, Positive; -, negative; w, weakly positive; v, variable results among different species.…”

Sulfitobacter aestuariivivens sp. nov., isolated from a tidal flat

“…The genus Sulfitobacter is characterized by enclosing Gram-negative, aerobic, catalase- and oxidase-positive species that have Q-10 and C 18 : 1 ω 7 c as their predominant ubiquinone and fatty acid, respectively [4–6]. Members of the genus Sulfitobacter have been isolated from Antarctic lakes, seawater, tidal flat, marine sand and marine organisms [2, 4–15]. During a screening of novel bacteria from a tidal flat on the Yellow Sea of the Republic of Korea, many novel bacterial taxa were isolated and characterized taxonomically.…”

“…At the time of writing, the genus Sulfitobacter comprises 22 species with validly published names (https://lpsn.dsmz.de/genus/sulfitobacter) [3]. The genus Sulfitobacter is characterized by enclosing Gram-negative, aerobic, catalase- and oxidase-positive species that have Q-10 and C 18 : 1 ω 7 c as their predominant ubiquinone and fatty acid, respectively [4–6]. Members of the genus Sulfitobacter have been isolated from Antarctic lakes, seawater, tidal flat, marine sand and marine organisms [2, 4–15].…”

Characterization of multidrug resistance in Vibrio species isolated from marine invertebrates from Andaman Sea

“…The diameter of each antimicrobial disc was 6 mm. Strains were considered susceptible when the inhibition zone diameter was greater than 8 mm (Kumari et al 2016). All the experiments were performed in triplicates.…”