Sulfitobacter geojensis sp. nov., Sulfitobacter noctilucae sp. nov., and Sulfitobacter noctilucicola sp. nov., isolated from coastal seawater

Abstract

Four Gram-stain-negative, aerobic, rod-shaped bacterial strains, MM-124, MM-126, NB-68 and NB-77, were isolated from the coastal seawater or a region with a bloom of sea sparkle around Geoje island in Korea. The sequence similarity values of the 16S rRNA gene between the isolates and Sulfitobacter mediterraneus DSM 12244T ranged from 97.7 to 98.2 %, and phylogenetic relationships suggested that they belong to a phylogenetic branch that includes the genera Sulfitobacter and Roseobacter . The isoprenoid quinone of all three novel strains was ubiquinone-10 and the major fatty acid was cis-vaccenic acid, as in other species of the genus Sulfitobacter . However, there were several differences in the morphological, physiological and biochemical characteristics among the four strains and the reference species of the genus Sulfitobacter . Moreover, the average nucleotide identity values between the three sequenced isolates and the reference strains were below 76.33, indicating that genomic variation exists between the isolates and reference strains. Chemotaxonomic characteristics together with phylogenetic affiliations and genomic distances illustrate that strains MM-124, NB-68 and NB-77 represent novel species of the genus Sulfitobacter , for which the names Sulfitobacter geojensis sp. nov. (type strain MM-124T = KCTC 32124T = JCM 18835T), Sulfitobacter noctilucae sp. nov. (type strain NB-68T = KCTC 32122T = JCM 18833T) and Sulfitobacter noctilucicola sp. nov. (type strain NB-77T = KCTC 32123T = JCM 18834T) are proposed.

Characteristics and Evolutionary analysis of Photosynthetic Gene Clusters on Extrachromosomal Replicons: from streamlined plasmids to chromids

“…Culture-independent surveys of AAPBs have indicated that Sulfitobacter account for a significant fraction of AAPB communities in natural environments (Buchan et al , 2005; Boeuf et al , 2013; Yinxin Zeng et al , 2016). However, only a few Sulfitobacter AAPB strains have been isolated and described (Labrenz et al , 2000; Boeuf et al , 2013; Kwak et al , 2014). Genomes from only three Sulfitobacter AAPB strains have been sequenced, which has revealed that their PGCs were located on ECRs.…”

Antarcticimicrobium sediminis gen. nov., sp. nov., isolated from Antarctic intertidal sediment, transfer of Ruegeria lutea to Antarcticimicrobium gen. nov. as Antarcticimicrobium luteum comb. nov.

“…n. microbium a Table 2. Differential characteristics of the genus Antarcticimicrobium and closely related genera in the family Rhodobacteraceae Genera: 1, Antarcticimicrobium gen. nov. (strain S4J41 T and Ruegeria lutea JCM 30927 T , this study); 2, Ruegeria [30,[37][38][39][40][41][42][43][44][45][46][47][48][49][50][51]; 3, Phaeobacter (this study and [30,[52][53][54][55][56]); 4, Puniceibacterium (this study and [5,57]); 5, Sulfitobacter (this study and [58][59][60][61][62][63][64][65][66][67][68][69][70][71][72][73]); 6, Pseudopuniceibacterium [36]. +, Positive; -, negative; w, weakly positive; v, variable results among different species.…”

Sulfitobacter faviae sp. nov., isolated from the coral Favia veroni

“…The DNA G+C content varies between 55 and 63.7 mol%. Based on 16S rRNA gene sequence analysis, members of this genus are closely related to the monotypic genus Oceanibulbus (Wagner-Döbler et al, 2004) T in the phylogenetic tree (Ivanova et al, 2004;Kwak et al, 2014;Fukui et al, 2014).In the present study, we describe the phenotypic, genotypic and chemotaxonomic characteristics of three strains, i.e. S5-53 T , S6-62 and S6-64, isolated from mucus of the coral Favia veroni from the Andaman Sea, India.…”

“…The DNA G+C content varies between 55 and 63.7 mol%. Based on 16S rRNA gene sequence analysis, members of this genus are closely related to the monotypic genus Oceanibulbus (Wagner-Döbler et al, 2004) T in the phylogenetic tree (Ivanova et al, 2004;Kwak et al, 2014;Fukui et al, 2014).…”

“…The genus was introduced by Sorokin (1995) to accommodate two strains of heterotrophic bacteria isolated from the Black Sea with Sulfitobacter pontiacus as the type species. At the time of writing, the genus Sulfitobacter contains 15 recognized species (Parte, 2014) that were isolated from marine environments either from seawater (Sorokin, 1995;Kwak et al, 2014) or associated with marine invertebrates (Fukui et al, 2014;Hong et al, 2015) from different geographical regions and thought to play an important role in organic sulfur cycling in the ocean environment (Ivanova et al, 2004). Phenotypically, strains are Gram-negative, aerobic and rod-shaped.…”