Sulfoacidibacillus ferrooxidans, gen. nov., sp. nov., Sulfoacidibacillus thermotolerans, gen. nov., sp. nov., and Ferroacidibacillus organovorans, gen. nov., sp. nov.: Extremely acidophilic chemolitho-heterotrophic Firmicutes

Johnson, D. Barrie; Holmes, David S.; Vergara, Eva; Holanda, Roseanne; Pakostová, Eva

doi:10.1016/j.resmic.2022.104008

Cited by 9 publications

(14 citation statements)

References 30 publications

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“…The Firmicutes are an extremely diverse phylum of bacteria, with a plethora of possible ecological roles. Acid-tolerant, sulfate-reducing and sulfide-producing Firmicutes have been described previously ( Holanda et al, 2016 ; Holanda and Johnson, 2020 ; Johnson et al, 2023 ), yet many remain uncharacterized. In common, most Firmicutes are endospores formers ( Galperin, 2013 ), so it is likely that several of the taxa identified in the sediments of Lake Caviahue are dormant and/or metabolically inactive cells.…”

Section: Discussionmentioning

confidence: 99%

Nutrient structure dynamics and microbial communities at the water–sediment interface in an extremely acidic lake in northern Patagonia

Cuevas,

Francisco,

Díaz-González

et al. 2024

Front. Microbiol.

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Lake Caviahue (37° 50 ‘S and 71° 06’ W; Patagonia, Argentina) is an extreme case of a glacial, naturally acidic, aquatic environment (pH ~ 3). Knowledge of the bacterial communities in the water column of this lake, is incipient, with a basal quantification of the bacterioplankton abundance distribution in the North and South Basins of Lake Caviahue, and the described the presence of sulfur and iron oxidizing bacteria in the lake sediments. The role that bacterioplankton plays in nutrient utilization and recycling in this environment, especially in the phosphorus cycle, has not been studied. In this work, we explore this aspect in further depth by assessing the diversity of pelagic, littoral and sediment bacteria, using state of the art molecular methods and identifying the differences and commonalties in the composition of the cognate communities. Also, we investigate the interactions between the sediments of Lake Caviahue and the microbial communities present in both sediments, pore water and the water column, to comprehend the ecological relationships driving nutrient structure and fluxes, with a special focus on carbon, nitrogen, and phosphorus. Two major environmental patterns were observed: (a) one distinguishing the surface water samples due to temperature, Fe2+, and electrical conductivity, and (b) another distinguishing winter and summer samples due to the high pH and increasing concentrations of N-NH4+, DOC and SO42−, from autumn and spring samples with high soluble reactive phosphorus (SRP) and iron concentrations. The largest bacterial abundance was found in autumn, alongside higher levels of dissolved phosphorus, iron forms, and increased conductivity. The highest values of bacterial biomass were found in the bottom strata of the lake, which is also where the greatest diversity in microbial communities was found. The experiments using continuous flow column microcosms showed that microbial growth over time, in both the test and control columns, was accompanied by a decrease in the concentration of dissolved nutrients (SRP and N-NH4+), providing proof that sediment microorganisms are active and contribute significantly to nutrient utilization/mobilization.

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Section: Discussionmentioning

confidence: 99%

Nutrient structure dynamics and microbial communities at the water–sediment interface in an extremely acidic lake in northern Patagonia

Cuevas,

Francisco,

Díaz-González

et al. 2024

Front. Microbiol.

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“…Illumina amplicon sequencing of 16S rRNA genes identified three chemolithotrophic bacteria that oxidize Fe 2+ in the bioleachate collected at the end of the flask experiment (on day 63); Acidithiobacillus accounted for 98.4% of total reads, while Leptospirillum and Sulfoacidibacillus (former Acidibacillus) [29,30] were present with low abundances (≤0.2%). Additionally, the human pathogen Bordetella was detected in the leachate with a low abundance (0.2%).…”

Section: Bioleaching Of Cu Using Acidophilic Fe 2+ -Oxidizing Consortiummentioning

confidence: 99%

“…The proportion of Acidithiobacillus gradually increased during Cu bioleaching, reaching 26.3 and 29.4% of total reads (and Leptospirillum accounting for 65.9 and 50.0%) at the end of each phase of the bioleaching step (i.e., on days 9 and 15, respectively). The only other genus known to contain species capable of Fe 2+ oxidation was Sulfoacidibacillus (formerly Acidibacillus) [29,30], accounting for 0.1% of total reads in the inoculum and 3.6% at the end of the pre-growth phase. Other genera detected were minor contaminants (accounting for <5% of total reads).…”

Section: Bioleaching Of Cu In a Bioreactor Using Adapted Acidophilic ...mentioning

confidence: 99%

A Bioleaching Process for Sustainable Recycling of Complex Structures with Multi-Metal Layers

Pakostova,

Herath

2023

Sustainability

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Industrial waste is accumulating, while primary metal resources are depleting. Bioleaching has been shown to be a cost-effective and environmentally friendly approach to metal recovery from waste, but improved designs are needed for large-scale recycling. Metal components that are manufactured by electrodeposition over a mandrel can be difficult to recycle using conventional techniques due to their complex geometry and inner Ag coating. A sustainable biotechnology for separating Cu and Ag from waste electrodeposited components is presented. Two-step bioleaching experiments were performed, during which Cu was solubilized by Fe3+ regenerated by Acidithiobacillus (At.) ferrooxidans CF3 and a consortium of ten acidophilic Fe2+-oxidizers. High Cu recovery rates were achieved in agitated flasks (22 °C, pH 1.9), with At. ferrooxidans solubilizing 94.7% Cu in 78 days and the consortium 99.2% Cu in 59 days. Copper bio-solubilization was significantly accelerated in a laboratory-scale bioreactor (32 °C, 1 L air min−1) using the bacterial consortium adapted to elevated Cu concentrations, reaching >99.6% Cu extraction in only 12 days. The bioreactor was dominated by Leptospirillum and Acidithiobacillus, with their proportions changing (from 83.2 to 59% of total reads and from 3.6 to 29.4%, respectively) during the leaching process. Dissolved Cu was recovered from the bioleachates (containing 14 to 22 g Cu L−1) using electrowinning; >99% of the Cu was deposited (with Cu purity of 98.5 to 99.9%) in 3.33 h (at current efficiency between 80 and 92%). The findings emphasize the importance of a bioleaching system design to achieve economical separation of base and precious metals from industrial wastes. The presented technology minimizes waste generation and energy consumption. On a larger scale, it has the potential to contribute to the development of industrial recycling processes that will protect natural resources and contribute to the Net Zero target.

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“…The family Alicyclobacillaceae, within the class Bacilli, was originally described by da Costa M.S., et al [1], and later emended by Klenk et al [2]. Currently, the family comprises seven genera, including Alicyclobacillus [3], Tumebacillus [4], Kyrpidia [2], Effusibacillus [5], Collibacillus [6], Sulfoacidibacillus, and Ferroacidibacillus [7]. Members of this family inhabit a variety of environments, such as permafrost [4], soil [8], copper mines, acidic water and sediment [7,9], as well as beverages [10], and potentially play important roles in the improvement of metallurgical efficiency, the biogeochemical cycling of iron elements in acidic environments, and even the fermentation process of beverages.…”

Section: Introductionmentioning

confidence: 99%

“…Most species are aerobic and chemoorganotrophic, utilizing organic compounds as their sole carbon and energy sources. However, a few strains can reduce nitrate to nitrite, and some can even reduce Fe (III) [7,11,12]. Some species can grow mixotrophically using Fe 2+ , S 0 , and sulfide minerals as electron donors in the presence of yeast extract as a sole organic compound [7,13].…”

Section: Introductionmentioning

confidence: 99%

Fodinisporobacter ferrooxydans gen. nov., sp. nov.—A Spore-Forming Ferrous-Oxidizing Bacterium Isolated from a Polymetallic Mine

Jiang,

Li,

Liang

et al. 2024

Microorganisms

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A novel acidophilic, aerobic bacterium strain, MYW30-H2T, was isolated from a heap of polymetallic mine. Cells of strain MYW30-H2T were Gram-stain-positive, endospore-forming, motile, and rod-shaped. Strain MYW30-H2T grew at a temperature range of 30–45 °C (optimum 40 °C) and a pH range of 3.5–6.0 (optimum 4.0) in the presence of 0–0.5% (w/v) NaCl. Strain MYW30-H2T could grow heterotrophically on yeast extract and glucose, and grow mixotrophically using ferrous iron as an electron donor with yeast extract. Menaquinone-7 (MK-7) was the sole respiratory quinone of the strain. Iso-C15:0 and anteiso-C15:0 were the major cellular fatty acids. The 16S rRNA gene sequence analysis showed that MYW30-H2T was phylogenetically affiliated with the family Alicyclobacillaceae, and the sequence similarity with other Alicyclobacillaceae genera species was below 91.51%. The average amino acid identity value of the strain with its phylogenetically related species was 52.3–62.1%, which fell into the genus boundary range. The DNA G+C content of the strain was 44.2%. Based on physiological and phylogenetic analyses, strain MYW30-H2T represents a novel species of a new genus of the family Alicyclobacillaceae, for which the name Fodinisporobacter ferrooxydans gen. nov., sp. nov. is proposed. The type strain is MYW30-H2T (=CGMCC 1.17422T = KCTC 43278T).

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Sulfoacidibacillus ferrooxidans, gen. nov., sp. nov., Sulfoacidibacillus thermotolerans, gen. nov., sp. nov., and Ferroacidibacillus organovorans, gen. nov., sp. nov.: Extremely acidophilic chemolitho-heterotrophic Firmicutes

Cited by 9 publications

References 30 publications

Nutrient structure dynamics and microbial communities at the water–sediment interface in an extremely acidic lake in northern Patagonia

Nutrient structure dynamics and microbial communities at the water–sediment interface in an extremely acidic lake in northern Patagonia

A Bioleaching Process for Sustainable Recycling of Complex Structures with Multi-Metal Layers

Fodinisporobacter ferrooxydans gen. nov., sp. nov.—A Spore-Forming Ferrous-Oxidizing Bacterium Isolated from a Polymetallic Mine

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